P73460 (RSMH_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	sll1144

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP-Rule MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP-Rule MF_01007
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA base methylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	rRNA (cytosine-N4-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Ribosomal RNA small subunit methyltransferase H HAMAP-Rule MF_01007		PRO_0000108731
Regions
	Region	49 – 51	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	68	1	S-adenosyl-L-methionine By similarity
	Binding site	100	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	116	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P73460 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 758F9F567247129F	FASTA	305	34,386
	10 20 30 40 50 60 MGAKSMEGMN LKQTAIADFH HVSVLPTALV EGLEPKPGGY YLDATVGAGG HSERLLKLGI 70 80 90 100 110 120 PLALTMIDRD VQAIAAAMER LQPLVADRKD ISINTWQGNF ADFPGQLEQF DGIIADLGVS 130 140 150 160 170 180 SPQLDQGDRG FSFRHTAPLD MRMDQSQDLT AAEIINHWSE KDLARIFYEY GEERLSRRIA 190 200 210 220 230 240 RQIVEQRPFQ TTTDLAEAIT KWVPGKYRHG RIHPATRTFQ GLRIAVNDEL GSLEKFIAQA 250 260 270 280 290 300 PHWLKSGGKF GIISFHSLED RIVKHRFRET ENLRVLTKKP IIAGEEEQRQ NPRARSAKLR WAEKI

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA17500.1.
PIR	S77397.
RefSeq	YP_007450703.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P73460.
ModBase	Search...
Protein-protein interaction databases
STRING	1148.sll1144.
Proteomic databases
PaxDb	P73460.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA17500; BAA17500; BAA17500.
GeneID	14616355.
Phylogenomic databases
eggNOG	COG0275.
OMA	NPATRAF.
ProtClustDB	PRK00050.
Family and domain databases
Gene3D	1.10.150.170. 1 hit.
HAMAP	MF_01007. 16SrRNA_methyltr_H.
InterPro	IPR002903. RsmH. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
PANTHER	PTHR11265. PTHR11265. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_SYNY3

Accession

Primary (citable) accession number: P73460

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 1, 1997
Last modified:	May 29, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program