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Protein

Ribosomal RNA small subunit methyltransferase H

Gene

rsmH

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei68 – 681S-adenosyl-L-methionineUniRule annotation
Binding sitei100 – 1001S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei116 – 1161S-adenosyl-L-methionineUniRule annotation
Binding sitei123 – 1231S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

  1. rRNA (cytosine-N4-)-methyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. rRNA base methylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase HUniRule annotation (EC:2.1.1.199UniRule annotation)
Alternative name(s):
16S rRNA m(4)C1402 methyltransferaseUniRule annotation
rRNA (cytosine-N(4)-)-methyltransferase RsmHUniRule annotation
Gene namesi
Name:rsmHUniRule annotation
Synonyms:mraW
Ordered Locus Names:sll1144
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Ribosomal RNA small subunit methyltransferase HPRO_0000108731Add
BLAST

Proteomic databases

PaxDbiP73460.

Interactioni

Protein-protein interaction databases

STRINGi1148.sll1144.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 513S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RsmH family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0275.
InParanoidiP73460.
OMAiGCYLDGT.
PhylomeDBiP73460.

Family and domain databases

Gene3Di1.10.150.170. 1 hit.
3.40.50.150. 2 hits.
HAMAPiMF_01007. 16SrRNA_methyltr_H.
InterProiIPR002903. RsmH.
IPR023397. SAM-dep_MeTrfase_MraW_recog.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11265. PTHR11265. 1 hit.
PfamiPF01795. Methyltransf_5. 1 hit.
[Graphical view]
PIRSFiPIRSF004486. MraW. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
SSF81799. SSF81799. 1 hit.
TIGRFAMsiTIGR00006. TIGR00006. 1 hit.

Sequencei

Sequence statusi: Complete.

P73460-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAKSMEGMN LKQTAIADFH HVSVLPTALV EGLEPKPGGY YLDATVGAGG
60 70 80 90 100
HSERLLKLGI PLALTMIDRD VQAIAAAMER LQPLVADRKD ISINTWQGNF
110 120 130 140 150
ADFPGQLEQF DGIIADLGVS SPQLDQGDRG FSFRHTAPLD MRMDQSQDLT
160 170 180 190 200
AAEIINHWSE KDLARIFYEY GEERLSRRIA RQIVEQRPFQ TTTDLAEAIT
210 220 230 240 250
KWVPGKYRHG RIHPATRTFQ GLRIAVNDEL GSLEKFIAQA PHWLKSGGKF
260 270 280 290 300
GIISFHSLED RIVKHRFRET ENLRVLTKKP IIAGEEEQRQ NPRARSAKLR

WAEKI
Length:305
Mass (Da):34,386
Last modified:February 1, 1997 - v1
Checksum:i758F9F567247129F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17500.1.
PIRiS77397.
RefSeqiYP_007450703.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA17500; BAA17500; BAA17500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17500.1.
PIRiS77397.
RefSeqiYP_007450703.1. NC_020286.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1148.sll1144.

Proteomic databases

PaxDbiP73460.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA17500; BAA17500; BAA17500.

Phylogenomic databases

eggNOGiCOG0275.
InParanoidiP73460.
OMAiGCYLDGT.
PhylomeDBiP73460.

Family and domain databases

Gene3Di1.10.150.170. 1 hit.
3.40.50.150. 2 hits.
HAMAPiMF_01007. 16SrRNA_methyltr_H.
InterProiIPR002903. RsmH.
IPR023397. SAM-dep_MeTrfase_MraW_recog.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11265. PTHR11265. 1 hit.
PfamiPF01795. Methyltransf_5. 1 hit.
[Graphical view]
PIRSFiPIRSF004486. MraW. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
SSF81799. SSF81799. 1 hit.
TIGRFAMsiTIGR00006. TIGR00006. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiRSMH_SYNY3
AccessioniPrimary (citable) accession number: P73460
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: April 1, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.