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Protein

Nitrate reductase

Gene

narB

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:

Pathway:initrate reduction (denitrification)

This protein is involved in step 1 of the subpathway that synthesizes dinitrogen from nitrate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Nitrate reductase (narB)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway nitrate reduction (denitrification), which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dinitrogen from nitrate, the pathway nitrate reduction (denitrification) and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi14 – 141Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi17 – 171Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi21 – 211Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi56 – 561Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

UniPathwayiUPA00652; UER00706.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:narB
Ordered Locus Names:sll1454
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 714714Nitrate reductasePRO_0000063238Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiP73448. 7 interactions.

Structurei

3D structure databases

ProteinModelPortaliP73448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 70644Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031440.
InParanoidiP73448.
KOiK00367.
OMAiFTTDEMW.
OrthoDBiEOG6CVV7G.
PhylomeDBiP73448.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P73448-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSPAILPTT RTLCPYCGVG CGLEAVASPQ KSVVDAGHAH KIRGDRQHPS
60 70 80 90 100
SQGMVCVKGA TVMESMDKQR LLYPMFRSSL DQPWQQISWE AALEIVVDKI
110 120 130 140 150
QQVKQTLGVS GLCMYASGQM QTEDYYVAQK LFKGCLGTNN FDTNSRLCMS
160 170 180 190 200
SAVSAYSLSF GSDGPPCCYE DLEITDCAFL IGTNTADCHP IIFNRLRKHH
210 220 230 240 250
KQNPHVKLIV VDPRCTATAE VADLHLAINP GSDITLLHGI AYLLKKWNLI
260 270 280 290 300
DQKFIDNHTQ DFEQYCQVID HYPPEKVTQI CGISLEQLET AAHYWGNAKT
310 320 330 340 350
VLSLWSMGMN QSFQGTAKGR CLINLHLLTG QIGKPGSGPF SLTGQPNAMG
360 370 380 390 400
GREAGGLSHL LPGYRSIKNP QHRQEVEQLW QISPGRINPE PGLSAWEMFM
410 420 430 440 450
GLENQQVGFL WIVATNPVVS MPDLERVKKA LQQSTFTIHQ DAYSPTETAE
460 470 480 490 500
YAHLLLPAAQ WSEKTGTMTN SERRVTLSPA FRSPPGEARP DWEIFAEVGR
510 520 530 540 550
RLGFENQFNF VDSAAVHREY VQLTAERLCD QSGVSYEKLQ KLGPLQWPCR
560 570 580 590 600
QSDQESQLLS TKRLYTDYKF CTENGRANFC LDHSRGLAEP VDPNYPFVLT
610 620 630 640 650
NGRLYGHWHT QTRTGHIEKI KKMHPKPILE MHPKDAEKLG IKSQDLVAIK
660 670 680 690 700
SRRGSAQLEV LVTRAIAPGT VFMPMHWGFL WDDNAEVNSL THATACPISK
710
QPELKACAVN ITPV
Length:714
Mass (Da):79,632
Last modified:February 1, 1997 - v1
Checksum:i702AA57ED9E605AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17488.1.
PIRiS77385.

Genome annotation databases

EnsemblBacteriaiBAA17488; BAA17488; BAA17488.
KEGGisyy:SYNGTS_0914.
syz:MYO_19210.
PATRICi23838852. VBISynSp132158_0991.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17488.1.
PIRiS77385.

3D structure databases

ProteinModelPortaliP73448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP73448. 7 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA17488; BAA17488; BAA17488.
KEGGisyy:SYNGTS_0914.
syz:MYO_19210.
PATRICi23838852. VBISynSp132158_0991.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031440.
InParanoidiP73448.
KOiK00367.
OMAiFTTDEMW.
OrthoDBiEOG6CVV7G.
PhylomeDBiP73448.

Enzyme and pathway databases

UniPathwayiUPA00652; UER00706.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiNARB_SYNY3
AccessioniPrimary (citable) accession number: P73448
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 22, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.