P73448 (NARB_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrate reductase
EC=1.7.99.4

Gene names

Name:	narB
Ordered Locus Names:	sll1454

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster Potential. Molybdenum (molybdopterin).
Pathway	Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 1/4.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	denitrification pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 714	714	Nitrate reductase		PRO_0000063238
Sites
	Metal binding	14	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	17	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	21	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	56	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P73448 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 702AA57ED9E605AA	FASTA	714	79,632
	10 20 30 40 50 60 MDSPAILPTT RTLCPYCGVG CGLEAVASPQ KSVVDAGHAH KIRGDRQHPS SQGMVCVKGA 70 80 90 100 110 120 TVMESMDKQR LLYPMFRSSL DQPWQQISWE AALEIVVDKI QQVKQTLGVS GLCMYASGQM 130 140 150 160 170 180 QTEDYYVAQK LFKGCLGTNN FDTNSRLCMS SAVSAYSLSF GSDGPPCCYE DLEITDCAFL 190 200 210 220 230 240 IGTNTADCHP IIFNRLRKHH KQNPHVKLIV VDPRCTATAE VADLHLAINP GSDITLLHGI 250 260 270 280 290 300 AYLLKKWNLI DQKFIDNHTQ DFEQYCQVID HYPPEKVTQI CGISLEQLET AAHYWGNAKT 310 320 330 340 350 360 VLSLWSMGMN QSFQGTAKGR CLINLHLLTG QIGKPGSGPF SLTGQPNAMG GREAGGLSHL 370 380 390 400 410 420 LPGYRSIKNP QHRQEVEQLW QISPGRINPE PGLSAWEMFM GLENQQVGFL WIVATNPVVS 430 440 450 460 470 480 MPDLERVKKA LQQSTFTIHQ DAYSPTETAE YAHLLLPAAQ WSEKTGTMTN SERRVTLSPA 490 500 510 520 530 540 FRSPPGEARP DWEIFAEVGR RLGFENQFNF VDSAAVHREY VQLTAERLCD QSGVSYEKLQ 550 560 570 580 590 600 KLGPLQWPCR QSDQESQLLS TKRLYTDYKF CTENGRANFC LDHSRGLAEP VDPNYPFVLT 610 620 630 640 650 660 NGRLYGHWHT QTRTGHIEKI KKMHPKPILE MHPKDAEKLG IKSQDLVAIK SRRGSAQLEV 670 680 690 700 710 LVTRAIAPGT VFMPMHWGFL WDDNAEVNSL THATACPISK QPELKACAVN ITPV

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA17488.1.
PIR	S77385.
RefSeq	NP_440808.1. NC_000911.1. YP_005650867.1. NC_017277.1. YP_007450691.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P73448.
ModBase	Search...
Protein-protein interaction databases
IntAct	P73448. 7 interactions.
STRING	1148.sll1454.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA17488; BAA17488; BAA17488.
GeneID	12254871. 14616343. 954111.
KEGG	syn:sll1454. syy:SYNGTS_0914.
PATRIC	23838852. VBISynSp132158_0991.
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HOG000031440.
KO	K00367.
OMA	RQHPSSQ.
ProtClustDB	CLSK892855.
Enzyme and pathway databases
UniPathway	UPA00652; UER00706.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. [Graphical view]
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NARB_SYNY3

Accession

Primary (citable) accession number: P73448

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program