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Protein

Lysine--tRNA ligase

Gene

lysS

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi415 – 4151Magnesium 1By similarity
Metal bindingi422 – 4221Magnesium 1By similarity
Metal bindingi422 – 4221Magnesium 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligase (EC:6.1.1.6)
Alternative name(s):
Lysyl-tRNA synthetase
Short name:
LysRS
Gene namesi
Name:lysS
Ordered Locus Names:slr1550
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 510510Lysine--tRNA ligasePRO_0000152695Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP73443. 5 interactions.

Structurei

3D structure databases

ProteinModelPortaliP73443.
SMRiP73443. Positions 461-499.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000236578.
InParanoidiP73443.
KOiK04567.
OMAiDMMNLTE.
OrthoDBiEOG69PQ2M.
PhylomeDBiP73443.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PIRSFiPIRSF039101. LysRS2. 1 hit.
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P73443-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSHSHSSL EEIRATRLEK AEQLRQLGLN PYAYTWEITH QAQDLQETYR
60 70 80 90 100
DLSNGEEVDL KVAIAGRILA RRVMGKLAFF TLQDESGTIQ LYLEKQRLTE
110 120 130 140 150
HMPELENAFN LLKKITDVGD ILGVTGTLKR TEKGELSVYV QTYAVLTKSL
160 170 180 190 200
LPLPDKWHGL TDTEKRYRQR YVDLIVNPTV RQTFRRRAQI TAAIRRYLDK
210 220 230 240 250
QGFIEIETPV LQGESGGAEA RPFITHHNTL GMPLYLRIAT ELHLKRLVVG
260 270 280 290 300
GFEKVFELGR IFRNEGVSTR HNPEFTSIEV YQAYVDYNEM MALTEALVTT
310 320 330 340 350
AAQAVLGTLK ITYQGEEIDL TPPWKRITMH EAVQLETGID FSQFTDLETA
360 370 380 390 400
KQAATKAGIG VPEDCPSLGH LLNHAFEQKV EGTLMQPTFI IDFPVEISPL
410 420 430 440 450
AKPHRSKPGL VERFELFVYG RELANSFSEL TDPIDQRSRL EAQAAKKAAG
460 470 480 490 500
DLEAHSVDED FLTALEYGMP PTGGLGIGID RLVMLLTDSP SIRDVIAFPL
510
LKNQEAGSDG
Length:510
Mass (Da):57,295
Last modified:February 1, 1997 - v1
Checksum:iF5897E92F0686704
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17483.1.
PIRiS77380.

Genome annotation databases

EnsemblBacteriaiBAA17483; BAA17483; BAA17483.
KEGGisyn:slr1550.
PATRICi23838838. VBISynSp132158_0984.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17483.1.
PIRiS77380.

3D structure databases

ProteinModelPortaliP73443.
SMRiP73443. Positions 461-499.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP73443. 5 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA17483; BAA17483; BAA17483.
KEGGisyn:slr1550.
PATRICi23838838. VBISynSp132158_0984.

Phylogenomic databases

HOGENOMiHOG000236578.
InParanoidiP73443.
KOiK04567.
OMAiDMMNLTE.
OrthoDBiEOG69PQ2M.
PhylomeDBiP73443.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PIRSFiPIRSF039101. LysRS2. 1 hit.
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiSYK_SYNY3
AccessioniPrimary (citable) accession number: P73443
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 16, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.