P73443 (SYK_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysine--tRNA ligase
EC=6.1.1.6

Alternative name(s):
Lysyl-tRNA synthetase
Short name=LysRS

Gene names

Name:	lysS
Ordered Locus Names:	slr1550

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP-Rule MF_00252
Cofactor	Binds 3 magnesium ions per subunit By similarity. HAMAP-Rule MF_00252
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00252
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00252.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Lysine--tRNA ligase HAMAP-Rule MF_00252		PRO_0000152695
Sites
	Metal binding	415	1	Magnesium 1 By similarity
	Metal binding	422	1	Magnesium 1 By similarity
	Metal binding	422	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P73443 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: F5897E92F0686704	FASTA	510	57,295
	10 20 30 40 50 60 MADSHSHSSL EEIRATRLEK AEQLRQLGLN PYAYTWEITH QAQDLQETYR DLSNGEEVDL 70 80 90 100 110 120 KVAIAGRILA RRVMGKLAFF TLQDESGTIQ LYLEKQRLTE HMPELENAFN LLKKITDVGD 130 140 150 160 170 180 ILGVTGTLKR TEKGELSVYV QTYAVLTKSL LPLPDKWHGL TDTEKRYRQR YVDLIVNPTV 190 200 210 220 230 240 RQTFRRRAQI TAAIRRYLDK QGFIEIETPV LQGESGGAEA RPFITHHNTL GMPLYLRIAT 250 260 270 280 290 300 ELHLKRLVVG GFEKVFELGR IFRNEGVSTR HNPEFTSIEV YQAYVDYNEM MALTEALVTT 310 320 330 340 350 360 AAQAVLGTLK ITYQGEEIDL TPPWKRITMH EAVQLETGID FSQFTDLETA KQAATKAGIG 370 380 390 400 410 420 VPEDCPSLGH LLNHAFEQKV EGTLMQPTFI IDFPVEISPL AKPHRSKPGL VERFELFVYG 430 440 450 460 470 480 RELANSFSEL TDPIDQRSRL EAQAAKKAAG DLEAHSVDED FLTALEYGMP PTGGLGIGID 490 500 510 RLVMLLTDSP SIRDVIAFPL LKNQEAGSDG

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA17483.1.
PIR	S77380.
RefSeq	NP_440803.1. NC_000911.1. YP_005650862.1. NC_017277.1. YP_007450686.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P73443.
SMR	P73443. Positions 461-499.
ModBase	Search...
Protein-protein interaction databases
IntAct	P73443. 5 interactions.
STRING	1148.slr1550.
Proteomic databases
PaxDb	P73443.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA17483; BAA17483; BAA17483.
GeneID	12254866. 14616338. 954106.
KEGG	syn:slr1550. syy:SYNGTS_0909.
PATRIC	23838838. VBISynSp132158_0984.
Phylogenomic databases
eggNOG	COG1190.
HOGENOM	HOG000236578.
KO	K04567.
OMA	VTFHNAL.
ProtClustDB	PRK00484.
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
HAMAP	MF_00252. Lys_tRNA_synth_class2.
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR002313. Lys-tRNA-ligase_II. IPR018149. Lys-tRNA-synth_II_C. IPR012340. NA-bd_OB-fold. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
PANTHER	PTHR22594. PTHR22594. 1 hit. PTHR22594:SF4. PTHR22594:SF4. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00499. lysS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK_SYNY3

Accession

Primary (citable) accession number: P73443

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program