Reviewed, UniProtKB/Swiss-Prot P73410 (CYSK_SYNY3)

Names and origin

Protein names

Recommended name:
    Cysteine synthase
      Short name=CSase
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase
    O-acetylserine (thiol)-lyase
      Short name=OAS-TL

Gene names

Name:	cysK
Ordered Locus Names:	slr1842

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Cysteine synthase		PRO_0000167104
Regions
	Region	181 – 185	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	77	1	Pyridoxal phosphate By similarity
	Binding site	269	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	46	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P73410-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 54D4DF4F5613EFC8	FASTA	312	33,173
	10 20 30 40 50 60 MKIASNITEL IGRTPLVRLN RIPLLEGCGA KIVVKLEGMN PAASVKDRIG INMINRAEQE 70 80 90 100 110 120 GLIEPGKTLL IEPTSGNTGI ALAMVAAAKG YQLILTMPET MSQERRAMLK AYGAKLELTP 130 140 150 160 170 180 GSEGMGGCIR RAQELAESLP NAYMLQQFDN PANPQIHQQT TALEIWQDTD GAIDFLVAGV 190 200 210 220 230 240 GTGGTITGVA SVLKEKKPSF QAIAVEPQNS PVLSGGKPGP HKIQGIGAGF IPEVLDVNLI 250 260 270 280 290 300 DEVIAVTDEE AIAYGRRLAR EEGILSGIST GAALAAAIKV AKRPANKDKL IVMIQPSFGE 310 RYLSTPLFQD LE

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA17450.1. Different initiation.
PIR	S77347.
RefSeq	NP_440770.1.
3D structure databases
HSSP	HSSP built from PDB template 1OAS based on UniProtKB P12674.
ModBase	Search...
Protein-protein interaction databases
IntAct	P73410. 7 interactions.
STRING	P73410.
Genome annotation databases
GeneID	954073.
GenomeReviews	Gene locus slr1842 in contig BA000022_GR.
KEGG	syn:slr1842.
NMPDR	fig|1148.1.peg.871.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P73410.
Enzyme and pathway databases
BioCyc	SSP1148:SLR1842-MON.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
TIGRFAMs	TIGR01139. cysK. 1 hit. TIGR01136. cysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK_SYNY3

Accession

Primary (citable) accession number: P73410

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	September 1, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)