Reviewed, UniProtKB/Swiss-Prot P73334 (RPOC2_SYNY3)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase subunit beta'
      Short name=RNAP subunit beta'
    EC=2.7.7.6
Alternative name(s):
    Transcriptase subunit beta'
    RNA polymerase subunit beta'

Gene names

Name:	rpoC2
Ordered Locus Names:	sll1789

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	1317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01324
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01324
Subunit structure	In cyanobacteria the RNAP catalytic core is composed of 2 alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family. RpoC2 subfamily.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
trxA	P52231	1	EBI-862802,EBI-862916

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1317	1317	DNA-directed RNA polymerase subunit beta' HAMAP MF_01324		PRO_0000067910

Sequences

	Sequence		Length	Mass (Da)
	P73334-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 27B6970469E7E551	FASTA	1,317	144,777
	10 20 30 40 50 60 MTFYNYTIDK GRLKKLIALA YRRYGSARCS QLADELKELG FRFATKAGVS ISVDDLTIPP 70 80 90 100 110 120 EKKQMLEAAE KEIRTTEERY ARGEITEVER FQKVIDTWNG TSEELKDQVV VNFRKTDPLN 130 140 150 160 170 180 SVYMMAFSGA RGNMSQVRQL VGMRGLMADP QGEIIDLPIK TNFREGLTVT EYVISSYGAR 190 200 210 220 230 240 KGLVDTALRT ADSGYLTRRL VDVSQDVIVR EQDCGTERSL RVTAMTDGDQ VKISLADRLF 250 260 270 280 290 300 GRLLAKDVVG PDGEIIAKRN DEIDEALANR IAAVTDEVYV RSPLTCEAAR SVCQNCYGWS 310 320 330 340 350 360 LAHGHKVDLG EAVGIIAAQS IGEPGTQLTM RTFHTGGVFT GEVARQEKAP EDGTVKWGKG 370 380 390 400 410 420 LSTRKVRTRH GEDAEQVEIA GDLIWKGEGK KAATQTYSLT PGSLLFVQDG QTVTAGQLMT 430 440 450 460 470 480 EISLSKTQRS TERATKDVAG DLAGEVLFDR LVPEEKTDRQ GNTTRIAQRG GLVWILSGEV 490 500 510 520 530 540 YNLPPGAEPV VKNDEQVEVG SIMAETKLVT NDGGVVRLVS NREIEIITAS VLLDQAQVKL 550 560 570 580 590 600 ESSGGREQYV IYTADKQRFL LKAAPGTKVQ NHSIVAELID DRYRTTTGGM IRYAGVEVAK 610 620 630 640 650 660 GGRKQGYEVT KGGTLLWIPE ETHEINKDIS LLIVEDGQYV EAGTEVVKDI FCQSSGIVEV 670 680 690 700 710 720 VQKNDILREI IIKPGDFYQD VDPGSVKIES GQLLQPGQDV FPGVTVSTLS QAEWIESPEG 730 740 750 760 770 780 NGLLLRPVEE YKVFDEPAAP SQGSQNEEGG RQIELRSVQR LFYKDGDRVK SVEGAPLLST 790 800 810 820 830 840 QLVLEIYGSG NEGISHLSAD IELQDDEEED CQRLQLVILE SLVLRRDQES DPLGGASKTR 850 860 870 880 890 900 LLVQDGDQIP PGAVVARTEI QCKEAGTVRG IKEGQESIRR VLLERAADRL VVDLPSAPEV 910 920 930 940 950 960 KPGQLLVAGQ ELVPGVKLEE SGKVLEINGK GDNYQLVLRR ARPYRVSPGA VLHIEDGDLV 970 980 990 1000 1010 1020 QRGDNLVLLV FERAKTGDIV QGLPRIEELL EARKPKEACV LARAPGVCQV EYLEDESVDI 1030 1040 1050 1060 1070 1080 KVVEDDGTVS EYPLLPGQNA MVTDGQRIDV GHALTDGYNN PHEILDVFFS YYVDKDGCYQ 1090 1100 1110 1120 1130 1140 AALRGLQAAQ KFLVNEVQTV YQSQGVDISD KHIEVIVRQM TAKVRIDDGG DTTMLPGELV 1150 1160 1170 1180 1190 1200 ELRQVEQVNE AMGITGSAPA RYTPVLLGIT KASLNTDSFI SAASFQETTR VLTEAAIEGK 1210 1220 1230 1240 1250 1260 SDWLRGLKEN VIIGRLIPAG TGFSSHEEVL GLIETQDDIQ GYMIEPIELP TTKKKASATK 1270 1280 1290 1300 1310 VKTKKVEADD DLLDDTRARA YAGTQLSQDD EEFEETYDTD EDDFDMDDDD DFGDDED

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA17364.1.
PIR	S77517.
RefSeq	NP_440684.1.
3D structure databases
HSSP	HSSP built from PDB template 1HQM based on UniProtKB Q9KWU6.
ModBase	Search...
Protein-protein interaction databases
IntAct	P73334. 2 interactions.
Genome annotation databases
GeneID	953987.
GenomeReviews	Gene locus sll1789 in contig BA000022_GR.
KEGG	syn:sll1789.
NMPDR	fig|1148.1.peg.785.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P73334.
OMA	P73334. IEGKSDW.
Enzyme and pathway databases
BioCyc	SSP1148:SLL1789-MON.
Family and domain databases
HAMAP	MF_01324. [Tree]
InterPro	IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. IPR012756. RpoC2_beta_pp. [Graphical view]
Pfam	PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
TIGRFAMs	TIGR02388. rpoC2_cyan. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOC2_SYNY3

Accession

Primary (citable) accession number: P73334

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)