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P73274 (SYL_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:sll1074
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length869 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 869869Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152105

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif622 – 6265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P73274 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 09D9380506F08D16

FASTA86998,295
        10         20         30         40         50         60 
MASPYNAGEI EQKWQQRWAE WGLDKTPIAS DLPKFYALSM FPYPSGNLHM GHVRNYTITD 

        70         80         90        100        110        120 
VIARLKRMQG YQVLHPMGWD AFGLPAENAA IERGIPPKQW TEKNIAQMRA QLQQLGLSID 

       130        140        150        160        170        180 
WEREVATCAP DYYRWTQWLF LEFFQAGLAY QKEATVNWDP IDQTVLANEQ VDSEGRSWRS 

       190        200        210        220        230        240 
GAMVERKLLR QWFLKITDYA EALLNDLEQL TGWPERVKLM QSHWIGKSVG AYLEFPIKDS 

       250        260        270        280        290        300 
QEKIAVFTTR PDTVYGVTYV VLAPEHPLTK VVTTAEQQGT VDEFVAMVAK ESEIERTAED 

       310        320        330        340        350        360 
KPKRGVKTGG IAINPFNGEE IPILIADYVL YEYGTGAVMG VPAHDQRDFK FAQDNNLPMQ 

       370        380        390        400        410        420 
VVIIPDDADN SDVNLTVAYT EAGVMVNSAQ FTGMASPKAK QAIIKFAEDN DYGRAKVQYR 

       430        440        450        460        470        480 
LRDWLISRQR YWGCPIPIIH CDDCGAVPVP TKDLPVELPD NVEFSGRGPS PLAKLEDWIN 

       490        500        510        520        530        540 
VPCPSCGKPA RRETDTMDTF IDSSWYFLRY ADAQNTELPF DGEKVAHWLP VDQYVGGIEH 

       550        560        570        580        590        600 
AILHLLYSRF FTKVLADRQL IPVKEPFQKL LTQGMVQGIT YKNPTTGKYV PAKDLQTGQQ 

       610        620        630        640        650        660 
VIDPKDPKDP DSGEPLQVFY EKMSKSKFNG VDPQEVLAKY GADTARMFIL FKAPPEKDLE 

       670        680        690        700        710        720 
WDDADVEGQF RFLNRVWRLV TDYIGDPAGI RFAVRPETLV TNEPLTKAEK DLRRAIHGAI 

       730        740        750        760        770        780 
KEVAEDLNDD YQFNTAISEM MKLSNALIAA TDLISFPVYQ EGIETLLLLL APFAPHLTEE 

       790        800        810        820        830        840 
LWHRLGRTDS IHQQAWLQVD PTALVLDEIT LVIQVLGKTR GTITVPASAD KTQLEELARN 

       850        860 
SDLAQRYLEG KTIKKVIVVP GKLVNFVIT 

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA17302.1.
PIRS77455.
RefSeqNP_440622.1. NC_000911.1.
YP_005650680.1. NC_017277.1.
YP_007450505.1. NC_020286.1.

3D structure databases

ProteinModelPortalP73274.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING1148.sll1074.

Proteomic databases

PaxDbP73274.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA17302; BAA17302; BAA17302.
GeneID953925.
KEGGsyn:sll1074.
syy:SYNGTS_0727.
syz:MYO_17330.
PATRIC23838442. VBISynSp132158_0788.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAPVIHCPK.
OrthoDBEOG63Z74X.
PhylomeDBP73274.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SYNY3
AccessionPrimary (citable) accession number: P73274
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries