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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei625 – 6251ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:sll1074
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 869869Leucine--tRNA ligasePRO_0000152105Add
BLAST

Proteomic databases

PaxDbiP73274.

Interactioni

Protein-protein interaction databases

STRINGi1148.sll1074.

Structurei

3D structure databases

ProteinModelPortaliP73274.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi622 – 6265"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
InParanoidiP73274.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.
PhylomeDBiP73274.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P73274-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASPYNAGEI EQKWQQRWAE WGLDKTPIAS DLPKFYALSM FPYPSGNLHM
60 70 80 90 100
GHVRNYTITD VIARLKRMQG YQVLHPMGWD AFGLPAENAA IERGIPPKQW
110 120 130 140 150
TEKNIAQMRA QLQQLGLSID WEREVATCAP DYYRWTQWLF LEFFQAGLAY
160 170 180 190 200
QKEATVNWDP IDQTVLANEQ VDSEGRSWRS GAMVERKLLR QWFLKITDYA
210 220 230 240 250
EALLNDLEQL TGWPERVKLM QSHWIGKSVG AYLEFPIKDS QEKIAVFTTR
260 270 280 290 300
PDTVYGVTYV VLAPEHPLTK VVTTAEQQGT VDEFVAMVAK ESEIERTAED
310 320 330 340 350
KPKRGVKTGG IAINPFNGEE IPILIADYVL YEYGTGAVMG VPAHDQRDFK
360 370 380 390 400
FAQDNNLPMQ VVIIPDDADN SDVNLTVAYT EAGVMVNSAQ FTGMASPKAK
410 420 430 440 450
QAIIKFAEDN DYGRAKVQYR LRDWLISRQR YWGCPIPIIH CDDCGAVPVP
460 470 480 490 500
TKDLPVELPD NVEFSGRGPS PLAKLEDWIN VPCPSCGKPA RRETDTMDTF
510 520 530 540 550
IDSSWYFLRY ADAQNTELPF DGEKVAHWLP VDQYVGGIEH AILHLLYSRF
560 570 580 590 600
FTKVLADRQL IPVKEPFQKL LTQGMVQGIT YKNPTTGKYV PAKDLQTGQQ
610 620 630 640 650
VIDPKDPKDP DSGEPLQVFY EKMSKSKFNG VDPQEVLAKY GADTARMFIL
660 670 680 690 700
FKAPPEKDLE WDDADVEGQF RFLNRVWRLV TDYIGDPAGI RFAVRPETLV
710 720 730 740 750
TNEPLTKAEK DLRRAIHGAI KEVAEDLNDD YQFNTAISEM MKLSNALIAA
760 770 780 790 800
TDLISFPVYQ EGIETLLLLL APFAPHLTEE LWHRLGRTDS IHQQAWLQVD
810 820 830 840 850
PTALVLDEIT LVIQVLGKTR GTITVPASAD KTQLEELARN SDLAQRYLEG
860
KTIKKVIVVP GKLVNFVIT
Length:869
Mass (Da):98,295
Last modified:February 1, 1997 - v1
Checksum:i09D9380506F08D16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17302.1.
PIRiS77455.
RefSeqiNP_440622.1. NC_000911.1.
YP_005650680.1. NC_017277.1.
YP_007450505.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA17302; BAA17302; BAA17302.
GeneIDi953925.
KEGGisyn:sll1074.
PATRICi23838442. VBISynSp132158_0788.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17302.1.
PIRiS77455.
RefSeqiNP_440622.1. NC_000911.1.
YP_005650680.1. NC_017277.1.
YP_007450505.1. NC_020286.1.

3D structure databases

ProteinModelPortaliP73274.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1148.sll1074.

Proteomic databases

PaxDbiP73274.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA17302; BAA17302; BAA17302.
GeneIDi953925.
KEGGisyn:sll1074.
PATRICi23838442. VBISynSp132158_0788.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
InParanoidiP73274.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.
PhylomeDBiP73274.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiSYL_SYNY3
AccessioniPrimary (citable) accession number: P73274
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 7, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.