ID   DFRA_SYNY3              Reviewed;         343 AA.
AC   P73212;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   27-MAR-2024, entry version 123.
DE   RecName: Full=Putative dihydroflavonol 4-reductase;
DE            Short=DFR;
DE            EC=1.1.1.219;
DE   AltName: Full=Dihydrokaempferol 4-reductase;
GN   Name=dfrA; OrderedLocusNames=slr1706;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteriota; Cyanophyceae; Synechococcales; Merismopediaceae;
OC   Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (2R,3S,4S)-leucoanthocyanidin + NADP(+) = a (2R,3R)-
CC         dihydroflavonol + H(+) + NADPH; Xref=Rhea:RHEA:54444,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:138176, ChEBI:CHEBI:138188; EC=1.1.1.219;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. Dihydroflavonol-4-reductase subfamily. {ECO:0000305}.
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DR   EMBL; BA000022; BAA17239.1; -; Genomic_DNA.
DR   PIR; S75325; S75325.
DR   AlphaFoldDB; P73212; -.
DR   SMR; P73212; -.
DR   IntAct; P73212; 1.
DR   STRING; 1148.gene:10498102; -.
DR   PaxDb; 1148-1652316; -.
DR   EnsemblBacteria; BAA17239; BAA17239; BAA17239.
DR   KEGG; syn:slr1706; -.
DR   eggNOG; COG0451; Bacteria.
DR   InParanoid; P73212; -.
DR   PhylomeDB; P73212; -.
DR   UniPathway; UPA00154; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0045552; F:dihydrokaempferol 4-reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IBA:GO_Central.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd05228; AR_FR_like_1_SDR_e; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR001509; Epimerase_deHydtase.
DR   InterPro; IPR017829; Hopanoid-assoc_sugar_epimerase.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   NCBIfam; TIGR03466; HpnA; 1.
DR   PANTHER; PTHR10366; NAD DEPENDENT EPIMERASE/DEHYDRATASE; 1.
DR   PANTHER; PTHR10366:SF624; NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN; 1.
DR   Pfam; PF01370; Epimerase; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE   3: Inferred from homology;
KW   Flavonoid biosynthesis; NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..343
FT                   /note="Putative dihydroflavonol 4-reductase"
FT                   /id="PRO_0000215573"
FT   BINDING         150
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:A0A059TC02"
SQ   SEQUENCE   343 AA;  37864 MW;  0008B4CC2EF971CA CRC64;
     MEVNHWIAMA DCFFVTGGTG FVGANLVRHL LEQGYQVRAL VRASSRPDNL QNLPIDWVVG
     DLNDGDLHQQ MQGCQGLFHV AAHYSLWQKD REALYRSNVL GTRNILACAQ KAGIERTVYT
     SSVAAIGVKG DGQRADESYQ SPVEKLIGAY KQSKYWAEQE ALTAAQQGQD IVIVNPSTPI
     GPWDIKPTPT GEIILRFLRR QMPAYVNTGL NLIDVRDVAA GHLLAWQRGK TALTRGDRYI
     LGHENISLQG ILAHLSTITG LPAPKNTVPL WLPLTFAWVE EKVLAPLGRS PSVPMDGVKM
     SAQEMYYDAS KAVQELGLPQ SSIKQALADA VHWFQNHGYV KTQ
//