Reviewed, UniProtKB/Swiss-Prot P73157 (LEP2_SYNY3)

Names and origin

Protein names

Recommended name:
    Probable signal peptidase I-2
      Short name=SPase I-2
    EC=3.4.21.89
Alternative name(s):
    Leader peptidase I-2

Gene names

Name:	lepB2
Ordered Locus Names:	slr1377

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Probable signal peptidase I-2		PRO_0000109536
Regions
	Topological domain	1 – 26	26	Cytoplasmic Potential
	Transmembrane	27 – 43	17	Potential
	Topological domain	44 – 218	175	Periplasmic Potential
Sites
	Active site	52	1	By similarity
	Active site	100	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P73157-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: F081C2AE157B20DD	FASTA	218	24,733
	10 20 30 40 50 60 MTENIVRETS KKKESPPENT WLELGKTMVT AVILAIGIRT FVAEARYIPS SSMEPTLQIN 70 80 90 100 110 120 DRLIIEKISY RLRDPERGEI VVFNPTDALK AKNFHDAFIK RIIGLPGDEV RVSQGNVYVN 130 140 150 160 170 180 GKMLDENYIA APPAYEYGPV KVPDDQYLVL GDNRNNSYDS HYWGFVPREK LLGRAFVRFW 190 200 210 PVPRVGLLTD DAEREAVEIS PQAWESPAIS PQTVPESR

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA17183.1.
PIR	S75269.
RefSeq	NP_440503.1.
3D structure databases
HSSP	HSSP built from PDB template 1B12 based on UniProtKB P00803.
ModBase	Search...
Protein-protein interaction databases
STRING	P73157.
Protein family/group databases
MEROPS	S26.008.
Genome annotation databases
GeneID	953804.
GenomeReviews	Gene locus slr1377 in contig BA000022_GR.
KEGG	syn:slr1377.
NMPDR	fig|1148.1.peg.604.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P73157.
OMA	AIGIRTF.
Enzyme and pathway databases
BioCyc	SSP1148:SLR1377-MON.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP2_SYNY3

Accession

Primary (citable) accession number: P73157

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 1, 1997
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)