P73141 (SYY_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	slr1031

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02007
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02007
Subunit structure	Homodimer By similarity. HAMAP MF_02007
Subcellular location	Cytoplasm By similarity HAMAP MF_02007.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Tyrosine--tRNA ligase HAMAP MF_02007		PRO_0000055664
Regions
	Domain	340 – 403	64	S4 RNA-binding
	Motif	49 – 58	10	"HIGH" region HAMAP MF_02007
	Motif	236 – 240	5	"KMSKS" region HAMAP MF_02007
Sites
	Binding site	239	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P73141 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: CA9EFA9041B7FF90	FASTA	404	44,901
	10 20 30 40 50 60 MVVNSDPDWL WRGTAEIFPH NADSNNPQEH LGILTQQDRP LRVKLGIDPT GTDIHLGHSI 70 80 90 100 110 120 PFRKLRAFQD AGHTAVVIIG DFTAQIGDPT GKSEVRKQLT ADQVKRNAES YLAQLKPILD 130 140 150 160 170 180 FDTPGRLEIR YNSEWLSNLD LNKILELLAT MTVGQMLAKE GFAERFQKEN PIFLHEFLYP 190 200 210 220 230 240 LMQGYDSVAV NADVELGGTD QKFNIAVGRD LQRHFGKTPQ FGLLLPILLG TDGQQKMSKS 250 260 270 280 290 300 LNNYVGLQED ALSMYSKLEK TPDRLLGEYF ELLTNLDLAE LPENPRECQK LLAKEVTAQF 310 320 330 340 350 360 HGVAGAIAAQ KTAEDIVTQG KAGNTDSVPE FSLAEITFPV KLAYLLSASG LCPSSSEGRR 370 380 390 400 QIKGGAVRLD GDRLEDVNQE YADPKMLINK VLQMGKKKFI RLIS

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA17167.1.
PIR	S75253.
RefSeq	NP_440487.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P73141.
ModBase	Search...
Protein-protein interaction databases
IntAct	P73141. 2 interactions.
STRING	P73141.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	953788.
GenomeReviews	Gene locus slr1031 in contig BA000022_GR.
KEGG	syn:slr1031.
NMPDR	fig|1148.1.peg.588.
PATRIC	23838158. VBISynSp132158_0646.
Phylogenomic databases
eggNOG	COG0162.
HOGENOM	HBG288125.
OMA	YVVQVGK.
PhylomeDB	P73141.
ProtClustDB	PRK05912.
Enzyme and pathway databases
BioCyc	SSP1148:SLR1031-MONOMER.
Family and domain databases
HAMAP	MF_02007. Tyr_tRNA_synth_type2. [Tree]
InterPro	IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-synth. IPR024088. Tyr-tRNA-synth_bac-type. IPR024108. Tyr-tRNA-synth_bac_2. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_SYNY3

Accession

Primary (citable) accession number: P73141

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program