P73138 (FRMA_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-(hydroxymethyl)glutathione dehydrogenase
EC=1.1.1.284

Alternative name(s):
Alcohol dehydrogenase class-3
EC=1.1.1.1
Alcohol dehydrogenase class-III
Glutathione-dependent formaldehyde dehydrogenase
Short name=FALDH
Short name=FDH
Short name=GSH-FDH
EC=1.1.1.-

Gene names

Name:	frmA
Ordered Locus Names:	sll0990

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H. An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ethanol oxidation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	S-(hydroxymethyl)glutathione dehydrogenase activity Inferred from electronic annotation. Source: EC alcohol dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	S-(hydroxymethyl)glutathione dehydrogenase		PRO_0000160778
Sites
	Metal binding	40	1	Zinc 1; catalytic By similarity
	Metal binding	62	1	Zinc 1; catalytic By similarity
	Metal binding	92	1	Zinc 2 By similarity
	Metal binding	95	1	Zinc 2 By similarity
	Metal binding	98	1	Zinc 2 By similarity
	Metal binding	106	1	Zinc 2 By similarity
	Metal binding	169	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P73138 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: C8A337424F2F1680	FASTA	369	39,211
	10 20 30 40 50 60 MKSRAAVAFE VGKPLQIVEI DVAPPQQGEV LVKITHTGVC HTDAFTLSGD DPEGLFPVVL 70 80 90 100 110 120 GHEGAGIVVE VGEGVTSVQL GDHVIPLYTA ECGKCLFCRS GKTNLCVAVR ATQGKGVMPD 130 140 150 160 170 180 GTSRFSYNGQ SLYHYMGCST FSEYTVVAEV SLAKINPEAN HEHVCLLGCG VTTGIGAVHN 190 200 210 220 230 240 TAKVQPGDSV AVFGLGGIGL AVVQGARQAK AGRIIAIDTN PAKFELAKQM GATDCINPKD 250 260 270 280 290 300 HDQPIQQVIV EMTGWGVDHS FECIGNVEVM RSALECAHRG WGQSVIIGVA GAGQEISTRP 310 320 330 340 350 360 FQLVTGRKWM GTAFGGVKGR SQLPGMVEQS MRGEIQLAPF VTHTMELKDI NQAFDLMHDG KSIRSVIHY

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA17164.1.
PIR	S75250.
RefSeq	NP_440484.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P73138.
SMR	P73138. Positions 1-367.
ModBase	Search...
Protein-protein interaction databases
STRING	P73138.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	953785.
GenomeReviews	Gene locus sll0990 in contig BA000022_GR.
KEGG	syn:sll0990.
NMPDR	fig|1148.1.peg.585.
PATRIC	23838146. VBISynSp132158_0641.
Phylogenomic databases
eggNOG	COG1062.
HOGENOM	HBG753318.
OMA	VDHTFEC.
PhylomeDB	P73138.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0990G-MONOMER.
Family and domain databases
InterPro	IPR014183. ADH_3. IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00121.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 2 hits.
TIGRFAMs	TIGR02818. Adh_III_F_hyde. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRMA_SYNY3

Accession

Primary (citable) accession number: P73138

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program