P73133 (ARGD_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylornithine aminotransferase
Short name=ACOAT
EC=2.6.1.11

Gene names

Name:	argD
Ordered Locus Names:	slr1022

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate. HAMAP-Rule MF_01107
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity. HAMAP-Rule MF_01107
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4. HAMAP-Rule MF_01107
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01107
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01107.
Miscellaneous	May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis. HAMAP-Rule MF_01107
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Acetylornithine aminotransferase HAMAP-Rule MF_01107		PRO_0000112804
Regions
	Region	251 – 254	4	Pyridoxal phosphate binding By similarity
Sites
	Binding site	160	1	Pyridoxal phosphate; via carbonyl oxygen By similarity
	Binding site	163	1	N2-acetyl-L-ornithine By similarity
	Binding site	307	1	N2-acetyl-L-ornithine By similarity
	Binding site	308	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	280	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P73133 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 99FAFA3ABE4AE530	FASTA	429	46,567
	10 20 30 40 50 60 MTYSPVVESV EAQAFAVTDL SPAAEFKTAD FDTYVMNTYG RFPIAIARGQ GSTLWDTEGK 70 80 90 100 110 120 SYLDFVAGIA TCTLGHAHPA LVRAVSDQIQ KLHHVSNLYY IPEQGELAKW IVEHSCADRV 130 140 150 160 170 180 FFCNSGAEAN EAAIKLVRKY AHTVLDFLEQ PVILTAKASF HGRTLATITA TGQPKYQQYF 190 200 210 220 230 240 DPLVPGFDYV PYNDIRSLEN KVADLDEGNS RVAAIFLEPL QGEGGVRPGD LAYFKRVREI 250 260 270 280 290 300 CDQNDILLVF DEVQVGVGRT GKLWGYEHLG VEPDIFTSAK GLAGGVPIGA MMCKKFCDVF 310 320 330 340 350 360 EPGNHASTFG GNPLACAAGL AVLKTIEGDR LLDNVQARGE QLRSGLAEIK NQYPTLFTEV 370 380 390 400 410 420 RGWGLINGLE ISAESSLTSV EIVKAAMEQG LLLAPAGPKV LRFVPPLVVT EAEIAQAVEI LRQAIATLV

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA17159.1.
PIR	S75245.
RefSeq	NP_440479.1. NC_000911.1. YP_005650537.1. NC_017277.1. YP_007450363.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P73133.
ModBase	Search...
Protein-protein interaction databases
IntAct	P73133. 2 interactions.
STRING	1148.slr1022.
Proteomic databases
PaxDb	P73133.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA17159; BAA17159; BAA17159.
GeneID	12256228. 14616012. 953780.
KEGG	syn:slr1022. syy:SYNGTS_0584.
PATRIC	23838136. VBISynSp132158_0636.
Phylogenomic databases
eggNOG	COG4992.
HOGENOM	HOG000020206.
KO	K00818.
OMA	FENPWQE.
ProtClustDB	PRK02627.
Enzyme and pathway databases
UniPathway	UPA00068; UER00109.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
HAMAP	MF_01107. ArgD_aminotrans_3.
InterPro	IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. IPR004636. Trfase_AcOrn/SuccOrn_fam. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF19. PTHR11986:SF19. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00707. argD. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGD_SYNY3

Accession

Primary (citable) accession number: P73133

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program