Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chaperone protein DnaJ 2

Gene

dnaJ2

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per monomer.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

DNA replication, Stress response

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJ 2
Gene namesi
Name:dnaJ2
Ordered Locus Names:sll1933
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000709161 – 307Chaperone protein DnaJ 2Add BLAST307

Proteomic databases

PRIDEiP73097.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP73097. 3 interactors.

Structurei

3D structure databases

ProteinModelPortaliP73097.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 71JPROSITE-ProRule annotationAdd BLAST66

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi70 – 74Gly-rich5

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity (By similarity).By similarity

Sequence similaritiesi

Belongs to the DnaJ family.Curated
Contains 1 J domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000226716.
InParanoidiP73097.
KOiK05516.
OMAiNIPTIDN.
PhylomeDBiP73097.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEiPS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P73097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQVRNYYQI LGVPRNATAE EIKKSFRKLA RQYHPDVNPN DKTAEEKFKD
60 70 80 90 100
INEAYDVLSD ETKRRELDSR LFGRFRRPPT SRFSPNSNGG RSPNGTSVNG
110 120 130 140 150
QVRTPTGRTG TRQPAQSWQD FSETRRTKVV SPARPVPRDV EANLTLPLEK
160 170 180 190 200
AYRGGKERIR LEDGRSLEVE MPGGMGDGQR IRLKQQGING GDLYLKINLS
210 220 230 240 250
PHPLFTLQGT DIACQVPVTP SEAILGGAIE VMTIDGLVKM TVPAGLKNGQ
260 270 280 290 300
KLRLAKKGFP NNQGDRGDQL VEIRVEIPPE PSPEELELYR RIREKETFNP

RQKFFDF
Length:307
Mass (Da):34,698
Last modified:February 1, 1997 - v1
Checksum:i1BF6525D723223EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17122.1.
PIRiS75208.

Genome annotation databases

EnsemblBacteriaiBAA17122; BAA17122; BAA17122.
KEGGisyn:sll1933.
PATRICi23838046. VBISynSp132158_0592.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17122.1.
PIRiS75208.

3D structure databases

ProteinModelPortaliP73097.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP73097. 3 interactors.

Proteomic databases

PRIDEiP73097.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA17122; BAA17122; BAA17122.
KEGGisyn:sll1933.
PATRICi23838046. VBISynSp132158_0592.

Phylogenomic databases

HOGENOMiHOG000226716.
InParanoidiP73097.
KOiK05516.
OMAiNIPTIDN.
PhylomeDBiP73097.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEiPS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNAJ2_SYNY3
AccessioniPrimary (citable) accession number: P73097
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.