P72933 (GLO2_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	sll1019

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_0000192356
Sites
	Metal binding	54	1	Zinc 1 By similarity
	Metal binding	56	1	Zinc 1 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	59	1	Zinc 2 By similarity
	Metal binding	113	1	Zinc 1 By similarity
	Metal binding	137	1	Zinc 1 By similarity
	Metal binding	137	1	Zinc 2 By similarity
	Metal binding	175	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P72933 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 34672EC7EB7B1734	FASTA	257	28,724
	10 20 30 40 50 60 MDIHRLPALA DNYIFLLHDR QRNQAAVVDP AEAKPVLDCL ETLGADLVTI YNTHHHGDHV 70 80 90 100 110 120 GANRELLAKY PNLEVYGGVE DQGRIPGQTV FLRDGDRLSF ADREATVYFV PGHTRGHIAY 130 140 150 160 170 180 YFAPGSGETI GDLFCGDTIF AGGCGRLFEG TPAQMVQSIG KLRQLPDQTR LWCAHEYTLG 190 200 210 220 230 240 NLKFALTVDP SNKDLQERFQ TVQGDRQRGQ ATIPSWLGTE KRTNPFLRWD NPAIQARVGM 250 TEPARVFGKL RGMKDNF

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA16950.1.
PIR	S74799.
RefSeq	NP_440270.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P72933.
ModBase	Search...
Protein-protein interaction databases
STRING	P72933.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	953569.
GenomeReviews	Gene locus sll1019 in contig BA000022_GR.
KEGG	syn:sll1019.
NMPDR	fig|1148.1.peg.371.
PATRIC	23837668. VBISynSp132158_0404.
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	IGCGRVF.
PhylomeDB	P72933.
Enzyme and pathway databases
BioCyc	SSP1148:SLL1019-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_SYNY3

Accession

Primary (citable) accession number: P72933

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program