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Protein

Hydroxyacylglutathione hydrolase

Gene

gloB

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.UniRule annotation

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable lactoylglutathione lyase (gloA)
  2. Hydroxyacylglutathione hydrolase (gloB)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Zinc 1UniRule annotation1
Metal bindingi56Zinc 1UniRule annotation1
Metal bindingi58Zinc 2UniRule annotation1
Metal bindingi59Zinc 2UniRule annotation1
Metal bindingi113Zinc 1UniRule annotation1
Metal bindingi137Zinc 1UniRule annotation1
Metal bindingi137Zinc 2UniRule annotation1
Metal bindingi175Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolaseUniRule annotation (EC:3.1.2.6UniRule annotation)
Alternative name(s):
Glyoxalase IIUniRule annotation
Short name:
Glx IIUniRule annotation
Gene namesi
Name:gloBUniRule annotation
Ordered Locus Names:sll1019
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001923561 – 257Hydroxyacylglutathione hydrolaseAdd BLAST257

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi1148.SYNGTS_0374.

Structurei

3D structure databases

ProteinModelPortaliP72933.
SMRiP72933.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000058041.
InParanoidiP72933.
KOiK01069.
OMAiNYIWLLQ.
PhylomeDBiP72933.

Family and domain databases

CDDicd07723. hydroxyacylglutathione_hydrola. 1 hit.
Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiView protein in InterPro
IPR035680. Clx_II_MBL.
IPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
PfamiView protein in Pfam
PF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiView protein in SMART
SM00849. Lactamase_B. 1 hit.
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequencei

Sequence statusi: Complete.

P72933-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIHRLPALA DNYIFLLHDR QRNQAAVVDP AEAKPVLDCL ETLGADLVTI
60 70 80 90 100
YNTHHHGDHV GANRELLAKY PNLEVYGGVE DQGRIPGQTV FLRDGDRLSF
110 120 130 140 150
ADREATVYFV PGHTRGHIAY YFAPGSGETI GDLFCGDTIF AGGCGRLFEG
160 170 180 190 200
TPAQMVQSIG KLRQLPDQTR LWCAHEYTLG NLKFALTVDP SNKDLQERFQ
210 220 230 240 250
TVQGDRQRGQ ATIPSWLGTE KRTNPFLRWD NPAIQARVGM TEPARVFGKL

RGMKDNF
Length:257
Mass (Da):28,724
Last modified:February 1, 1997 - v1
Checksum:i34672EC7EB7B1734
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA16950.1.
PIRiS74799.

Genome annotation databases

EnsemblBacteriaiBAA16950; BAA16950; BAA16950.
KEGGisyn:sll1019.

Similar proteinsi

Entry informationi

Entry nameiGLO2_SYNY3
AccessioniPrimary (citable) accession number: P72933
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: August 30, 2017
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names