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Protein

Hydroxyacylglutathione hydrolase

Gene

gloB

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.UniRule annotation

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathway: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable lactoylglutathione lyase (gloA)
  2. Hydroxyacylglutathione hydrolase (gloB)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Zinc 1UniRule annotation
Metal bindingi56 – 561Zinc 1UniRule annotation
Metal bindingi58 – 581Zinc 2UniRule annotation
Metal bindingi59 – 591Zinc 2UniRule annotation
Metal bindingi113 – 1131Zinc 1UniRule annotation
Metal bindingi137 – 1371Zinc 1UniRule annotation
Metal bindingi137 – 1371Zinc 2UniRule annotation
Metal bindingi175 – 1751Zinc 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolaseUniRule annotation (EC:3.1.2.6UniRule annotation)
Alternative name(s):
Glyoxalase IIUniRule annotation
Short name:
Glx IIUniRule annotation
Gene namesi
Name:gloBUniRule annotation
Ordered Locus Names:sll1019
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257Hydroxyacylglutathione hydrolasePRO_0000192356Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi1148.SYNGTS_0374.

Structurei

3D structure databases

ProteinModelPortaliP72933.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0491.
HOGENOMiHOG000058041.
InParanoidiP72933.
KOiK01069.
OMAiDNYIFLL.
OrthoDBiEOG69GZQ4.
PhylomeDBiP72933.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2.
InterProiIPR001279. Beta-lactamas-like.
IPR017782. Hydroxyacylglutathione_Hdrlase.
[Graphical view]
PANTHERiPTHR11935:SF7. PTHR11935:SF7. 1 hit.
PfamiPF00753. Lactamase_B. 1 hit.
[Graphical view]
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequencei

Sequence statusi: Complete.

P72933-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIHRLPALA DNYIFLLHDR QRNQAAVVDP AEAKPVLDCL ETLGADLVTI
60 70 80 90 100
YNTHHHGDHV GANRELLAKY PNLEVYGGVE DQGRIPGQTV FLRDGDRLSF
110 120 130 140 150
ADREATVYFV PGHTRGHIAY YFAPGSGETI GDLFCGDTIF AGGCGRLFEG
160 170 180 190 200
TPAQMVQSIG KLRQLPDQTR LWCAHEYTLG NLKFALTVDP SNKDLQERFQ
210 220 230 240 250
TVQGDRQRGQ ATIPSWLGTE KRTNPFLRWD NPAIQARVGM TEPARVFGKL

RGMKDNF
Length:257
Mass (Da):28,724
Last modified:February 1, 1997 - v1
Checksum:i34672EC7EB7B1734
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA16950.1.
PIRiS74799.
RefSeqiYP_005650327.1. NC_017277.1.
YP_007450153.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA16950; BAA16950; BAA16950.
KEGGisyn:sll1019.
PATRICi23837668. VBISynSp132158_0404.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA16950.1.
PIRiS74799.
RefSeqiYP_005650327.1. NC_017277.1.
YP_007450153.1. NC_020286.1.

3D structure databases

ProteinModelPortaliP72933.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1148.SYNGTS_0374.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA16950; BAA16950; BAA16950.
KEGGisyn:sll1019.
PATRICi23837668. VBISynSp132158_0404.

Phylogenomic databases

eggNOGiCOG0491.
HOGENOMiHOG000058041.
InParanoidiP72933.
KOiK01069.
OMAiDNYIFLL.
OrthoDBiEOG69GZQ4.
PhylomeDBiP72933.

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2.
InterProiIPR001279. Beta-lactamas-like.
IPR017782. Hydroxyacylglutathione_Hdrlase.
[Graphical view]
PANTHERiPTHR11935:SF7. PTHR11935:SF7. 1 hit.
PfamiPF00753. Lactamase_B. 1 hit.
[Graphical view]
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiGLO2_SYNY3
AccessioniPrimary (citable) accession number: P72933
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 24, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.