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Protein

Beta sliding clamp

Gene

dnaN

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Beta sliding clamp
Short name:
Beta clamp
Short name:
Sliding clamp
Alternative name(s):
Beta-clamp processivity factor
DNA polymerase III beta sliding clamp subunit
DNA polymerase III subunit beta
Gene namesi
Name:dnaN
Ordered Locus Names:slr0965
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

P72856:
  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001054751 – 391Beta sliding clampAdd BLAST391

Interactioni

Subunit structurei

Forms a ring-shaped head-to-tail homodimer around DNA which binds and tethers DNA polymerases and other proteins to the DNA. The DNA replisome complex has a single clamp-loading complex (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase.By similarity

Protein-protein interaction databases

STRINGi1148.SYNGTS_0295.

Structurei

3D structure databases

ProteinModelPortaliP72856.
SMRiP72856.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta sliding clamp family.Curated

Phylogenomic databases

HOGENOMiHOG000071791.
InParanoidiP72856.
KOiK02338.
OMAiNYEAVIP.
PhylomeDBiP72856.

Family and domain databases

CDDicd00140. beta_clamp. 1 hit.
InterProiView protein in InterPro
IPR001001. DNA_polIII_beta.
IPR022635. DNA_polIII_beta_C.
IPR022637. DNA_polIII_beta_cen.
IPR022634. DNA_polIII_beta_N.
PANTHERiPTHR30478. PTHR30478. 1 hit.
PfamiView protein in Pfam
PF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
PIRSFiPIRSF000804. DNA_pol_III_b. 1 hit.
SMARTiView protein in SMART
SM00480. POL3Bc. 1 hit.
TIGRFAMsiTIGR00663. dnan. 1 hit.

Sequencei

Sequence statusi: Complete.

P72856-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLICRQSDL SSGLSLVSRA VSSRPTHPVL GNVLLEADAD KNYLRLTAFD
60 70 80 90 100
LSLGIQSSFT ADVQQSGRIT LPAKLLNDIV SRLPDGDITL AIDPDGDAGD
110 120 130 140 150
SHLTTITSES GRFQIRGLDA DDFPALPTVE GVKPLLLPVA TLNEGLRGAL
160 170 180 190 200
FAASTDETKQ VLTGVHIKGS GDSLEFAATD GHRLAVVEAP TQIENDEGEA
210 220 230 240 250
VITGSDLADF AVTIPARALR ELERMVASQG NSDLVSLVVN DTQVIFELGD
260 270 280 290 300
QRLTSRKLEG AYPAYDQLIP RQFSRTVTME RKRLITSLER VSVLADQKNN
310 320 330 340 350
LVTFTLQSPG NQLQLAVEAQ DLGHGEESMG AEIIGEGGQI AFNIKYLMDG
360 370 380 390
LKALPSNDIQ MQLNEGNQPV IFTPLGGLKM TYLVMPVRLV N
Length:391
Mass (Da):42,088
Last modified:February 1, 1997 - v1
Checksum:i5FA9942EE1C7A7E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA16871.1.
PIRiS74720.

Genome annotation databases

EnsemblBacteriaiBAA16871; BAA16871; BAA16871.
KEGGisyn:slr0965.

Similar proteinsi

Entry informationi

Entry nameiDPO3B_SYNY3
AccessioniPrimary (citable) accession number: P72856
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 25, 2017
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names