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Protein

Beta sliding clamp

Gene

dnaN

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Beta sliding clamp
Short name:
Beta clamp
Short name:
Sliding clamp
Alternative name(s):
Beta-clamp processivity factor
DNA polymerase III beta sliding clamp subunit
DNA polymerase III subunit beta
Gene namesi
Name:dnaN
Ordered Locus Names:slr0965
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001054751 – 391Beta sliding clampAdd BLAST391

Proteomic databases

PaxDbiP72856
PRIDEiP72856

Interactioni

Subunit structurei

Forms a ring-shaped head-to-tail homodimer around DNA which binds and tethers DNA polymerases and other proteins to the DNA. The DNA replisome complex has a single clamp-loading complex (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase.By similarity

Protein-protein interaction databases

STRINGi1148.SYNGTS_0295

Structurei

3D structure databases

ProteinModelPortaliP72856
SMRiP72856
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta sliding clamp family.Curated

Phylogenomic databases

HOGENOMiHOG000071791
InParanoidiP72856
KOiK02338
OMAiNYEAVIP
PhylomeDBiP72856

Family and domain databases

CDDicd00140 beta_clamp, 1 hit
InterProiView protein in InterPro
IPR001001 DNA_polIII_beta
IPR022635 DNA_polIII_beta_C
IPR022637 DNA_polIII_beta_cen
IPR022634 DNA_polIII_beta_N
PANTHERiPTHR30478 PTHR30478, 1 hit
PfamiView protein in Pfam
PF00712 DNA_pol3_beta, 1 hit
PF02767 DNA_pol3_beta_2, 1 hit
PF02768 DNA_pol3_beta_3, 1 hit
PIRSFiPIRSF000804 DNA_pol_III_b, 1 hit
SMARTiView protein in SMART
SM00480 POL3Bc, 1 hit
TIGRFAMsiTIGR00663 dnan, 1 hit

Sequencei

Sequence statusi: Complete.

P72856-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLICRQSDL SSGLSLVSRA VSSRPTHPVL GNVLLEADAD KNYLRLTAFD
60 70 80 90 100
LSLGIQSSFT ADVQQSGRIT LPAKLLNDIV SRLPDGDITL AIDPDGDAGD
110 120 130 140 150
SHLTTITSES GRFQIRGLDA DDFPALPTVE GVKPLLLPVA TLNEGLRGAL
160 170 180 190 200
FAASTDETKQ VLTGVHIKGS GDSLEFAATD GHRLAVVEAP TQIENDEGEA
210 220 230 240 250
VITGSDLADF AVTIPARALR ELERMVASQG NSDLVSLVVN DTQVIFELGD
260 270 280 290 300
QRLTSRKLEG AYPAYDQLIP RQFSRTVTME RKRLITSLER VSVLADQKNN
310 320 330 340 350
LVTFTLQSPG NQLQLAVEAQ DLGHGEESMG AEIIGEGGQI AFNIKYLMDG
360 370 380 390
LKALPSNDIQ MQLNEGNQPV IFTPLGGLKM TYLVMPVRLV N
Length:391
Mass (Da):42,088
Last modified:February 1, 1997 - v1
Checksum:i5FA9942EE1C7A7E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA Translation: BAA16871.1
PIRiS74720

Genome annotation databases

EnsemblBacteriaiBAA16871; BAA16871; BAA16871
KEGGisyn:slr0965

Similar proteinsi

Entry informationi

Entry nameiDPO3B_SYNY3
AccessioniPrimary (citable) accession number: P72856
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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