Reviewed, UniProtKB/Swiss-Prot P72854 (SIR_SYNY3)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [ferredoxin]
    EC=1.8.7.1

Gene names

Name:	sir
Ordered Locus Names:	slr0963

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	635 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrogen sulfide + 6 oxidized ferredoxin + 3 H(2)O = sulfite + 6 reduced ferredoxin + 6 H(+).
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Thioether bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct sulfite reductase (ferredoxin) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
trxA	P52231	1	EBI-862835,EBI-862916

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 635	635	Sulfite reductase [ferredoxin]		PRO_0000199954
Sites
	Metal binding	451	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	457	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	496	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	500	1	Iron (siroheme axial ligand) By similarity
	Metal binding	500	1	Iron-sulfur (4Fe-4S) By similarity
Amino acid modifications
	Cross-link	54 ↔ 139		3'-(S-cysteinyl)-tyrosine (Tyr-Cys) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P72854-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 91F4A1B139AD7B34	FASTA	635	71,441
	10 20 30 40 50 60 MVTTPTAAPR KPSKVEGIKE RSNYLREPLA TELLNDANYF TDDAVQILKF HGSYQQDNRD 70 80 90 100 110 120 NRVKGQEKDY QFMLRTRNPG GLIPAQLYTA LDDLSKTHGN QTLRVTTRQG LQIHGIVKKD 130 140 150 160 170 180 LKMAIATVVN NLGSTLGACG DINRNVMAPA APFRDKPEYG YAWDYANKVA DLLSPQSGAY 190 200 210 220 230 240 YEIWLDGEKV ISGEEAPEVK AARQKDLNGT NLNDPKEPIY GQQFMPRKFK ISVTVPGDNS 250 260 270 280 290 300 IDVYTHDISL VVITDRHGEL RGFNVLAGGG LGRTHNKEET FARAADPIGY VSKDDVYDLV 310 320 330 340 350 360 KAIVATQRDY GDRHNRRHAR MKYLLADWGV EKFRKQVETY MGKPFQSFKP LPAWRYQDYL 370 380 390 400 410 420 GWHEQGDGKL FFGLSVENGR IKDEGDFQLK TALRKVVDQF QLPLRLTANH NILLYDINAQ 430 440 450 460 470 480 DKAAIEQIFQ QHGVVTDPEA IDTLVRYSMA CPALPTCGLA VTESERIMPS VNARLRDLLN 490 500 510 520 530 540 SLDLPNESIV TRMTGCPNGC ARPYMAEIGF VGSAPNSYQV WLGGSPNQER LAAAYTEKMP 550 560 570 580 590 600 LEQLESLFEP LFVYFKQSRK GKESFGDFCH RVGFTALREF SHGYTAPAKG GKNRKNQRRV 610 620 630 SLSDEMYAQL KARSERDNCP MNQIVQQALT AYLGK

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA16869.1.
PIR	S74718.
RefSeq	NP_440189.1.
3D structure databases
HSSP	HSSP built from PDB template 2GEP based on UniProtKB P17846.
ModBase	Search...
Protein-protein interaction databases
IntAct	P72854.
Genome annotation databases
GeneID	953488.
GenomeReviews	Gene locus slr0963 in contig BA000022_GR.
KEGG	syn:slr0963.
NMPDR	fig|1148.1.peg.290.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P72854.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0963-MON.
Family and domain databases
InterPro	IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR011787. Sir. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02042. sir. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SIR_SYNY3

Accession

Primary (citable) accession number: P72854

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 25, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)