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Protein

ATP-dependent 6-phosphofructokinase 1

Gene

pfkA1

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141ATP; via amide nitrogenUniRule annotation
Metal bindingi117 – 1171Magnesium; catalyticUniRule annotation
Sitei118 – 1181Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei142 – 1421Proton acceptorUniRule annotation
Binding sitei177 – 1771Substrate; shared with dimeric partnerUniRule annotation
Binding sitei237 – 2371SubstrateUniRule annotation
Binding sitei278 – 2781Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi79 – 802ATPUniRule annotation
Nucleotide bindingi116 – 1194ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 1UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 1UniRule annotation
Short name:
Phosphofructokinase 1UniRule annotation
Alternative name(s):
Phosphohexokinase 1UniRule annotation
Gene namesi
Name:pfkA1UniRule annotation
Synonyms:pfkA
Ordered Locus Names:sll1196
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361ATP-dependent 6-phosphofructokinase 1PRO_0000111998Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

IntActiP72830. 1 interaction.
STRINGi1148.sll1196.

Structurei

3D structure databases

ProteinModelPortaliP72830.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni140 – 1423Substrate bindingUniRule annotation
Regioni184 – 1863Substrate bindingUniRule annotation
Regioni284 – 2874Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
InParanoidiP72830.
KOiK00850.
OMAiRGLYNND.
OrthoDBiEOG644ZRM.
PhylomeDBiP72830.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P72830-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEIKRIGIL TSGGDCAGLN AVIRSVVHHA IGTYGWEVIG IQEATQGLME
60 70 80 90 100
NPSKAIALHR DNIDHLLMMG GTFLGTTNKG NPFAFPMADG TVRDRTEDII
110 120 130 140 150
AGYRQLGLDA LIGIGGDGSL AILRRIAQQG GINLVGIPKT IDNDVGATEI
160 170 180 190 200
SIGFDTATNI ATEALDRLHF TAASHNRVMV LEVMGRDAGH IALAAGIGGG
210 220 230 240 250
ADIILIPEIP YRIQSVCNKI RQRQAEGKNF CLVMVSEAVR TELGDQVKQI
260 270 280 290 300
QQFGEDRYGG IGKYIAEQIA QRTGAETRVT VLGHIQRGGI PSPFDRLLGS
310 320 330 340 350
VFGVAAVDLI AEGKFDHMVA WRNRQTISVP IEEAIQTYQT VQLDGTLVKT
360
ARGLGICLGN D
Length:361
Mass (Da):38,588
Last modified:February 1, 1997 - v1
Checksum:i96A83E05F318552C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA16845.1.
PIRiS74694.
RefSeqiNP_440165.1. NC_000911.1.
YP_005650222.1. NC_017277.1.
YP_007450048.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA16845; BAA16845; BAA16845.
GeneIDi953464.
KEGGisyn:sll1196.
PATRICi23837450. VBISynSp132158_0297.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA16845.1.
PIRiS74694.
RefSeqiNP_440165.1. NC_000911.1.
YP_005650222.1. NC_017277.1.
YP_007450048.1. NC_020286.1.

3D structure databases

ProteinModelPortaliP72830.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP72830. 1 interaction.
STRINGi1148.sll1196.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA16845; BAA16845; BAA16845.
GeneIDi953464.
KEGGisyn:sll1196.
PATRICi23837450. VBISynSp132158_0297.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
InParanoidiP72830.
KOiK00850.
OMAiRGLYNND.
OrthoDBiEOG644ZRM.
PhylomeDBiP72830.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiPFKA1_SYNY3
AccessioniPrimary (citable) accession number: P72830
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: January 7, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.