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P72830 (PFKA1_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase 1

Short name=ATP-PFK 1
Short name=Phosphofructokinase 1
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase 1
Gene names
Name:pfkA1
Synonyms:pfkA
Ordered Locus Names:sll1196
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_01976

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_01976

Cofactor

Magnesium By similarity. HAMAP-Rule MF_01976

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_01976

Subunit structure

Homodimer or homotetramer By similarity. HAMAP-Rule MF_01976

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01976.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 361361ATP-dependent 6-phosphofructokinase 1 HAMAP-Rule MF_01976
PRO_0000111998

Regions

Nucleotide binding79 – 802ATP By similarity
Nucleotide binding116 – 1194ATP By similarity
Region140 – 1423Substrate binding By similarity
Region184 – 1863Substrate binding By similarity
Region284 – 2874Substrate binding By similarity

Sites

Active site1421Proton acceptor By similarity
Metal binding1171Magnesium; catalytic By similarity
Binding site141ATP; via amide nitrogen By similarity
Binding site1771Substrate; shared with dimeric partner By similarity
Binding site2371Substrate By similarity
Binding site2781Substrate; shared with dimeric partner By similarity
Site1181Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity

Sequences

Sequence LengthMass (Da)Tools
P72830 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 96A83E05F318552C

FASTA36138,588
        10         20         30         40         50         60 
MGEIKRIGIL TSGGDCAGLN AVIRSVVHHA IGTYGWEVIG IQEATQGLME NPSKAIALHR 

        70         80         90        100        110        120 
DNIDHLLMMG GTFLGTTNKG NPFAFPMADG TVRDRTEDII AGYRQLGLDA LIGIGGDGSL 

       130        140        150        160        170        180 
AILRRIAQQG GINLVGIPKT IDNDVGATEI SIGFDTATNI ATEALDRLHF TAASHNRVMV 

       190        200        210        220        230        240 
LEVMGRDAGH IALAAGIGGG ADIILIPEIP YRIQSVCNKI RQRQAEGKNF CLVMVSEAVR 

       250        260        270        280        290        300 
TELGDQVKQI QQFGEDRYGG IGKYIAEQIA QRTGAETRVT VLGHIQRGGI PSPFDRLLGS 

       310        320        330        340        350        360 
VFGVAAVDLI AEGKFDHMVA WRNRQTISVP IEEAIQTYQT VQLDGTLVKT ARGLGICLGN 


D 

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References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA16845.1.
PIRS74694.
RefSeqNP_440165.1. NC_000911.1.
YP_005650222.1. NC_017277.1.
YP_007450048.1. NC_020286.1.

3D structure databases

ProteinModelPortalP72830.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP72830. 1 interaction.
STRING1148.sll1196.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA16845; BAA16845; BAA16845.
GeneID953464.
KEGGsyn:sll1196.
syy:SYNGTS_0269.
syz:MYO_12710.
PATRIC23837450. VBISynSp132158_0297.

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248869.
KOK00850.
OMANEICKAM.
OrthoDBEOG644ZRM.
PhylomeDBP72830.

Enzyme and pathway databases

UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_01976. Phosphofructokinase_III.
InterProIPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02483. PFK_mixed. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA1_SYNY3
AccessionPrimary (citable) accession number: P72830
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways