Reviewed, UniProtKB/Swiss-Prot P72830 (K6PF1_SYNY3)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase 1
    EC=2.7.1.11
Alternative name(s):
    Phosphofructokinase 1
    Phosphohexokinase 1

Gene names

Name:	pfkA1
Synonyms:	pfkA
Ordered Locus Names:	sll1196

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	6-phosphofructokinase 1 HAMAP MF_00339		PRO_0000111998
Regions
	Nucleotide binding	24 – 28	5	ATP By similarity
	Nucleotide binding	169 – 173	5	ATP By similarity
	Nucleotide binding	186 – 202	17	ATP By similarity
Sites
	Active site	142	1	Proton acceptor By similarity
	Metal binding	200	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	177	1	Substrate By similarity
	Binding site	278	1	Substrate By similarity
	Binding site	284	1	Substrate By similarity
	Binding site	287	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P72830-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 96A83E05F318552C	FASTA	361	38,588
	10 20 30 40 50 60 MGEIKRIGIL TSGGDCAGLN AVIRSVVHHA IGTYGWEVIG IQEATQGLME NPSKAIALHR 70 80 90 100 110 120 DNIDHLLMMG GTFLGTTNKG NPFAFPMADG TVRDRTEDII AGYRQLGLDA LIGIGGDGSL 130 140 150 160 170 180 AILRRIAQQG GINLVGIPKT IDNDVGATEI SIGFDTATNI ATEALDRLHF TAASHNRVMV 190 200 210 220 230 240 LEVMGRDAGH IALAAGIGGG ADIILIPEIP YRIQSVCNKI RQRQAEGKNF CLVMVSEAVR 250 260 270 280 290 300 TELGDQVKQI QQFGEDRYGG IGKYIAEQIA QRTGAETRVT VLGHIQRGGI PSPFDRLLGS 310 320 330 340 350 360 VFGVAAVDLI AEGKFDHMVA WRNRQTISVP IEEAIQTYQT VQLDGTLVKT ARGLGICLGN D

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA16845.1.
PIR	S74694.
RefSeq	NP_440165.1.
3D structure databases
HSSP	HSSP built from PDB template 3PFK based on UniProtKB P00512.
ModBase	Search...
Protein-protein interaction databases
IntAct	P72830. 1 interaction.
Genome annotation databases
GeneID	953464.
GenomeReviews	Gene locus sll1196 in contig BA000022_GR.
KEGG	syn:sll1196.
NMPDR	fig|1148.1.peg.266.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P72830.
OMA	P72830. ESHHRIM.
Enzyme and pathway databases
BioCyc	SSP1148:SLL1196-MON.
Family and domain databases
HAMAP	MF_00339. [Tree]
InterPro	IPR012003. ATP_PFK_prok. IPR012829. PFK. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
ProDom	PD000707. Ppfruckinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02483. PFK_mixed. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF1_SYNY3

Accession

Primary (citable) accession number: P72830

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)