Reviewed, UniProtKB/Swiss-Prot P72723 (DFA2_SYNY3)

Names and origin

Protein names

Recommended name:
    Putative diflavin flavoprotein A 2
    EC=1.-.-.-

Gene names

Name:	dfa2
Ordered Locus Names:	sll0217

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Mediates electron transfer from NADH to oxygen, reducing it to water. This modular protein has 3 redox cofactors, in other organisms the same activity requires 2 or 3 proteins By similarity.
Miscellaneous	By homology with norV in E.coli, may be involved in nitric oxide detoxification By similarity.
Sequence similarities	In the N-terminal section; belongs to the zinc metallo-hydrolase group 3 family. In the C-terminal section; belongs to the flavodoxin reductase family. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro hydrolase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 578	578	Putative diflavin flavoprotein A 2		PRO_0000216802
Regions
	Domain	269 – 406	138	Flavodoxin-like
	Region	48 – 240	193	Zinc metallo-hydrolase
	Region	429 – 578	150	Flavodoxin-reductase-like
Sites
	Metal binding	97	1	Iron 1 By similarity
	Metal binding	99	1	Iron 1 By similarity
	Metal binding	101	1	Iron 2 By similarity
	Metal binding	164	1	Iron 1 By similarity
	Metal binding	183	1	Iron 1 By similarity
	Metal binding	183	1	Iron 2 By similarity
	Metal binding	240	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P72723-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 88F0FF9E5EF7C938	FASTA	578	64,083
	10 20 30 40 50 60 MVTLIDSPTS AAVQPRLTLQ TADIAAHTTA IRCLDWDRDR FDIEFELRHG TTYNSFLIRG 70 80 90 100 110 120 EKTALIDTSH RKFEAVYLQL LQDLVDLRSL DYLIVNHTEP DHSGLIPDLL ELAPQVTVVG 130 140 150 160 170 180 SKVAIQFLEK LVHRPFESQI VKSGHSLDLG QGHELQFISA PNLHWPDTIL TYDSGTQVLY 190 200 210 220 230 240 TCDVFGMHYC DDSLFDETPE RLEPDFQYYY NCLMGSNARS VLMALKRIAP LQVVLVATGH 250 260 270 280 290 300 GPLLQHHISH WLGQYDAWSQ NQVKAETFVA LFYVDGYGVS DRLVQTIADG ISKTGVAIEL 310 320 330 340 350 360 VDLSVADTHE VRTLAQCAAG LVVGMPPQSS TSTTLDPLLG TILAAVHPKQ VIGLFESGGG 370 380 390 400 410 420 QDEPIYPLRN RFQELGLQEA FEPILLKTEP TAATDQLCRE AGTDLGQYLT QKQSQQANTD 430 440 450 460 470 480 LDPELNQAIG RLSTGLYILT AQKGDVRSAM LASWVIQGSF EPLGIVIAVA KDRAIESLLH 490 500 510 520 530 540 PGDTFVLNVL EEDNYQSLMR HFLLRFPPGA DRFAGVNTYP AQNGSPILLE TLAYLECEVT 550 560 570 SRLDGNDHWL VYSTIQTGRV AKLNALTATH HRKLGNHY

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA16730.1.
PIR	S74578.
RefSeq	NP_440050.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P72723. 1 interaction.
STRING	P72723.
Genome annotation databases
GeneID	953349.
GenomeReviews	Gene locus sll0217 in contig BA000022_GR.
KEGG	syn:sll0217.
NMPDR	fig|1148.1.peg.151.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284362.
OMA	GHELQFI.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0217-MON.
Family and domain databases
InterPro	IPR001279. Blactmase-like. IPR002563. Flavin_Rdtase_FMN_bd. IPR008254. Flavodoxin/NO_synth. IPR009002. Split_barrel_FMN-bd_related. IPR012349. Split_barrel_FMN_bd. [Graphical view]
Gene3D	G3DSA:2.30.110.10. PNPOx_FMN_bd. 1 hit.
Pfam	PF01613. Flavin_Reduct. 1 hit. [Graphical view]
SMART	SM00903. Flavin_Reduct. 1 hit. SM00849. Lactamase_B. 1 hit. [Graphical view]
PROSITE	PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DFA2_SYNY3

Accession

Primary (citable) accession number: P72723

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	February 1, 1997
Last modified:	December 15, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)