P72721 (DFA4_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative diflavin flavoprotein A 4
EC=1.-.-.-

Gene names

Name:	dfa4
Ordered Locus Names:	sll0219

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Mediates electron transfer from NADH to oxygen, reducing it to water. This modular protein has 3 redox cofactors, in other organisms the same activity requires 2 or 3 proteins By similarity.
Cofactor	Binds 2 iron ions per subunit.
Miscellaneous	By homology with norV in E.coli, may be involved in nitric oxide detoxification By similarity.
Sequence similarities	In the N-terminal section; belongs to the zinc metallo-hydrolase group 3 family. In the C-terminal section; belongs to the flavodoxin reductase family. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro flavin reductase activity Inferred from electronic annotation. Source: InterPro hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 594	594	Putative diflavin flavoprotein A 4		PRO_0000216804
Regions
	Domain	279 – 417	139	Flavodoxin-like
	Region	57 – 250	194	Zinc metallo-hydrolase
	Region	445 – 594	150	Flavodoxin-reductase-like

Sequences

	Sequence		Length	Mass (Da)
	P72721 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 70CD60E725BBED2D	FASTA	594	65,597
	10 20 30 40 50 60 MISPIGGLSQ ALHSPSDSIF VASLPRDIQV AEIAPQTKVL RSRLWDRLKF EVEYGRRRGT 70 80 90 100 110 120 TSNSYLIQAD HTALIDPPGE SFCDLYLAEL PKYLDLAQLD YIVASHVNPN RMVTLEQLLR 130 140 150 160 170 180 RATKAKLICS RPAAKVLKAT FPHWEERFQT VRSQDMLDLG RGHKLQLMTL PTPRWPDGLC 190 200 210 220 230 240 AYDAGSQILF SDKLFGTHVC GDAIFDEDWR QLGGDRRFYF DCLHAPQTRQ VETALDQFDP 250 260 270 280 290 300 LTLKMIAPGH GPLVRFSLSR LYSDYRQWCQ QQPSQTLKVA LIYASAYGNT ATMARAIAQG 310 320 330 340 350 360 LVKAGVAVET INCEIAEPNE IVEAIQACDG FIVGSPTLGS HAPVQIQTAL GIVLSSATKT 370 380 390 400 410 420 KLAGVFGSYG WSGEAIDLIE NKLKDGGYRF GFEAIRIQFS PNLDALDVCT TSGANFARQL 430 440 450 460 470 480 RTHKRQRIAR QATTETQADR TQQAVGRIIG SIGVVTTQTT GRHQGILTSW VSQASFTPPG 490 500 510 520 530 540 IMLAIPGEFD AYGLAGQNKA FVLNLLQEGR SVRRHFDHQP LPKDGDNPFS RLEHYSTQNG 550 560 570 580 590 CLILAEALAY LECLVQSWSN IGDHVLVYAT VQAGQVLQPN GITAIRHRKS GGQY

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA16728.1.
PIR	S74576.
RefSeq	NP_440048.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P72721.
ModBase	Search...
Protein-protein interaction databases
STRING	P72721.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	953347.
GenomeReviews	Gene locus sll0219 in contig BA000022_GR.
KEGG	syn:sll0219.
NMPDR	fig|1148.1.peg.149.
PATRIC	23837174. VBISynSp132158_0162.
Phylogenomic databases
eggNOG	COG0426.
HOGENOM	HBG284362.
OMA	AVETINC.
PhylomeDB	P72721.
ProtClustDB	CLSK892508.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0219-MONOMER.
Family and domain databases
InterPro	IPR001279. Beta-lactamas-like. IPR002563. Flavin_Rdtase_FMN-bd. IPR008254. Flavodoxin/NO_synth. IPR001226. Flavodoxin_CS. IPR012349. Split_barrel_FMN-bd. IPR009002. Split_barrel_FMN-bd-related. [Graphical view]
Gene3D	G3DSA:2.30.110.10. PNPOx_FMN_bd. 1 hit.
KO	K00540.
Pfam	PF01613. Flavin_Reduct. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00903. Flavin_Reduct. 1 hit. SM00849. Lactamase_B. 1 hit. [Graphical view]
SUPFAM	SSF50475. FMN_binding. 1 hit.
PROSITE	PS00201. FLAVODOXIN. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DFA4_SYNY3

Accession

Primary (citable) accession number: P72721

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program