Reviewed, UniProtKB/Swiss-Prot P72720 (GLMS_SYNY3)

Names and origin

Protein names

Recommended name:
    Glucosamine--fructose-6-phosphate aminotransferase [isomerizing]
    EC=2.6.1.16
Alternative name(s):
    Hexosephosphate aminotransferase
    D-fructose-6-phosphate amidotransferase
    GFAT
    L-glutamine-D-fructose-6-phosphate amidotransferase
    Glucosamine-6-phosphate synthase

Gene names

Name:	glmS
Ordered Locus Names:	sll0220

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	631 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source By similarity.
Catalytic activity	L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate. HAMAP MF_00164
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 glutamine amidotransferase type-2 domain. Contains 2 SIS domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase Repeat
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutamine-fructose-6-phosphate transaminase (isomerizing) activity Inferred from electronic annotation. Source: HAMAP sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 631	630	Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] HAMAP MF_00164		PRO_0000135399
Regions
	Domain	2 – 225	224	Glutamine amidotransferase type-2
	Domain	298 – 446	149	SIS 1
	Domain	480 – 621	142	SIS 2
Sites
	Active site	2	1	Nucleophile; for GATase activity By similarity
	Active site	626	1	For Fru-6P isomerization activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P72720-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: A681521E28918FA6	FASTA	631	69,607
	10 20 30 40 50 60 MCGIVGYIGT QTAVNILIEG LERLEYRGYD SAGIATVTEG KIESVRAKGK LFNLKEKLEN 70 80 90 100 110 120 HSNFSRLGIG HTRWATHGKP EEHNAHPHLD NQQRIAVVQN GIIENYQTLR DQLKEKGYQF 130 140 150 160 170 180 YSETDTEVIP ILIADILKDL PSDDPDEALL EAIGKAVHQL EGAFAIAVLD AHCPEQLIVA 190 200 210 220 230 240 RQQAPLILGF GQGEFFCASD VTALVHHTTT VLSLENGEIA RLTPLGVEVY DFNLKRVRKL 250 260 270 280 290 300 PRTLDWSATT VEKQGFRHFM LKEIYEQPAV VRTCLATYLN EQWRAADHPS HSPVFLGLDP 310 320 330 340 350 360 QLTKNLQHIQ VLACGTSWHA GLVGKYLLEQ LAGIPTTVHY ASEFRYAAPP LTPHTLTIGV 370 380 390 400 410 420 TQSGETADTL AALEMEKQRR LTLEDDYKPL ILGITNRPES TLATMVNEII NTHAGIEIGV 430 440 450 460 470 480 AATKTFVAQV LAFYFLALDI AFQRHSLSLE AIEHIMVGLR QLPAQIETIL EQQGSAIEAL 490 500 510 520 530 540 AHEFAETQDF IFLGRGINFP IALEGALKLK EISYIHAEGY PAGEMKHGPI ALLDAKVPVV 550 560 570 580 590 600 AIAMPGSVHD KVISNAQEAK ARDARLIGVT PMDDSQARSV FDDLLLVPHV EEMLSPIVAV 610 620 630 IPLQLLSYHI AARRGLDVDQ PRNLAKSVTV E

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA16727.1.
PIR	S74575.
RefSeq	NP_440047.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P72720.
Protein family/group databases
MEROPS	C44.971.
Genome annotation databases
GeneID	953346.
GenomeReviews	Gene locus sll0220 in contig BA000022_GR.
KEGG	syn:sll0220.
NMPDR	fig|1148.1.peg.148.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P72720.
OMA	WLETVAR
Enzyme and pathway databases
BioCyc	SSP1148:SLL0220-MON.
Family and domain databases
HAMAP	MF_00164. [Tree]
InterPro	IPR000583. GATase_2. IPR017932. GATase_II. IPR005855. GlmS_trans. IPR001347. SIS. [Graphical view]
Pfam	PF00310. GATase_2. 1 hit. PF01380. SIS. 2 hits. [Graphical view]
TIGRFAMs	TIGR01135. glmS. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS51464. SIS. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLMS_SYNY3

Accession

Primary (citable) accession number: P72720

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 59 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)