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Protein

NAD(P)H-quinone oxidoreductase subunit 2

Gene

ndhB

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient. Cyanobacterial NDH-1 also plays a role in inorganic carbon-concentration.UniRule annotation

Catalytic activityi

NAD(P)H + plastoquinone = NAD(P)+ + plastoquinol.UniRule annotation

GO - Molecular functioni

  1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
  2. quinone binding Source: UniProtKB-KW

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: InterPro
  2. photosynthesis, light reaction Source: UniProtKB-HAMAP
  3. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Transport

Keywords - Ligandi

NAD, NADP, Plastoquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H-quinone oxidoreductase subunit 2UniRule annotation (EC:1.6.5.-UniRule annotation)
Alternative name(s):
NAD(P)H dehydrogenase subunit 2UniRule annotation
NADH-plastoquinone oxidoreductase subunit 2UniRule annotation
NDH-1, subunit 2UniRule annotation
Gene namesi
Name:ndhBUniRule annotation
Ordered Locus Names:sll0223
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Cellular thylakoid membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei16 – 3621HelicalUniRule annotationAdd
BLAST
Transmembranei40 – 6021HelicalUniRule annotationAdd
BLAST
Transmembranei80 – 10021HelicalUniRule annotationAdd
BLAST
Transmembranei110 – 13021HelicalUniRule annotationAdd
BLAST
Transmembranei133 – 15321HelicalUniRule annotationAdd
BLAST
Transmembranei168 – 18821HelicalUniRule annotationAdd
BLAST
Transmembranei212 – 23221HelicalUniRule annotationAdd
BLAST
Transmembranei246 – 26621HelicalUniRule annotationAdd
BLAST
Transmembranei280 – 30021HelicalUniRule annotationAdd
BLAST
Transmembranei308 – 32821HelicalUniRule annotationAdd
BLAST
Transmembranei336 – 35621HelicalUniRule annotationAdd
BLAST
Transmembranei380 – 40021HelicalUniRule annotationAdd
BLAST
Transmembranei402 – 42221HelicalUniRule annotationAdd
BLAST
Transmembranei468 – 48821HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. thylakoid membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 521521NAD(P)H-quinone oxidoreductase subunit 2PRO_0000117685Add
BLAST

Keywords - PTMi

Quinone

Proteomic databases

PaxDbiP72714.

Interactioni

Subunit structurei

NDH-1 can be composed of about 15 different subunits; different subcomplexes with different compositions have been identified which probably have different functions.UniRule annotation

Protein-protein interaction databases

IntActiP72714. 9 interactions.
STRINGi1148.sll0223.

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 2 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1007.
HOGENOMiHOG000100794.
InParanoidiP72714.
KOiK05573.
OMAiYALILMT.
OrthoDBiEOG64JFNZ.
PhylomeDBiP72714.

Family and domain databases

HAMAPiMF_00445. NDH1_NuoN_1.
InterProiIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
[Graphical view]
PfamiPF00361. Oxidored_q1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01770. NDH_I_N. 1 hit.

Sequencei

Sequence statusi: Complete.

P72714-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFSSNVAAQ LNAGTILPEG IVIVTLLLVL IVDLIGGRKV ALALPYLAIA
60 70 80 90 100
GLLVSVGLLV TSWSMADPIG FIGAFNGDNL SIIFRAIIAL STVVTILMSV
110 120 130 140 150
RYVQQTGTSL AEFIAILLTA TLGGMFLSAA NELVMVFISL EMLSISSYLM
160 170 180 190 200
TGYMKRDPRS NEAALKYLLI GASSSAIFLY GLSLLYGLSG GETQLVLIAE
210 220 230 240 250
KLVNADTVGQ SLGLAIALVF VIAGIAFKIS AVPFHQWTPD VYEGSPTPVV
260 270 280 290 300
AFLSVGSKAA GFAVAIRLLV TAFGGITDEW HVIFTALAVL SMVLGNVVAL
310 320 330 340 350
AQTSMKRMLA YSSIGQAGFV MIGLVAGSED GYASMVFYML IYLFMNLGAF
360 370 380 390 400
SCIILFTLRT GSDQISDYAG LYHKDPLLTL GLSICLLSLG GIPPLAGFFG
410 420 430 440 450
KIYIFWAGWQ SGLYGLVLLG LVTSVVSIYY YIRVVKMMVV KEPQEMSEVI
460 470 480 490 500
KNYPAIKWNL PGMRPLQVGI VATLVATSLA GILANPLFNL ATDSVVSTKM
510 520
LQTALQQTGE TPAIAISHDL P
Length:521
Mass (Da):55,355
Last modified:February 1, 1997 - v1
Checksum:iC18C06704C277081
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti412 – 4121G → R in BAA14330 (PubMed:1903537).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90288 Genomic DNA. Translation: BAA14330.1.
BA000022 Genomic DNA. Translation: BAA16721.1.
PIRiS74569.
RefSeqiNP_440041.1. NC_000911.1.
YP_005650098.1. NC_017277.1.
YP_007449924.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA16721; BAA16721; BAA16721.
GeneIDi953340.
KEGGisyn:sll0223.
PATRICi23837162. VBISynSp132158_0156.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90288 Genomic DNA. Translation: BAA14330.1.
BA000022 Genomic DNA. Translation: BAA16721.1.
PIRiS74569.
RefSeqiNP_440041.1. NC_000911.1.
YP_005650098.1. NC_017277.1.
YP_007449924.1. NC_020286.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP72714. 9 interactions.
STRINGi1148.sll0223.

Proteomic databases

PaxDbiP72714.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA16721; BAA16721; BAA16721.
GeneIDi953340.
KEGGisyn:sll0223.
PATRICi23837162. VBISynSp132158_0156.

Phylogenomic databases

eggNOGiCOG1007.
HOGENOMiHOG000100794.
InParanoidiP72714.
KOiK05573.
OMAiYALILMT.
OrthoDBiEOG64JFNZ.
PhylomeDBiP72714.

Family and domain databases

HAMAPiMF_00445. NDH1_NuoN_1.
InterProiIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
[Graphical view]
PfamiPF00361. Oxidored_q1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01770. NDH_I_N. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A gene homologous to the subunit-2 gene of NADH dehydrogenase is essential to inorganic carbon transport of Synechocystis PCC6803."
    Ogawa T.
    Proc. Natl. Acad. Sci. U.S.A. 88:4275-4279(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiNU2C_SYNY3
AccessioniPrimary (citable) accession number: P72714
Secondary accession number(s): Q55269
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 7, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.