Reviewed, UniProtKB/Swiss-Prot P72714 (NU2C_SYNY3)

Names and origin

Protein names

Recommended name:
    NAD(P)H-quinone oxidoreductase chain 2
    EC=1.6.5.-
Alternative name(s):
    NAD(P)H dehydrogenase I, chain 2
    NDH-1, chain 2

Gene names

Name:	ndhB
Ordered Locus Names:	sll0223

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NAD(P)H, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. HAMAP MF_00445
Catalytic activity	NAD(P)H + plastoquinone = NAD(P)+ + plastoquinol. HAMAP MF_00445
Subcellular location	Cellular thylakoid membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the complex I subunit 2 family.

Ontologies

Keywords
Cellular component	Membrane Thylakoid
Domain	Transmembrane
Ligand	NAD NADP Plastoquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell thylakoid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 521	521	NAD(P)H-quinone oxidoreductase chain 2 HAMAP MF_00445		PRO_0000117685
Regions
	Transmembrane	16 – 36	21	Potential
	Transmembrane	40 – 60	21	Potential
	Transmembrane	80 – 100	21	Potential
	Transmembrane	110 – 130	21	Potential
	Transmembrane	133 – 153	21	Potential
	Transmembrane	168 – 188	21	Potential
	Transmembrane	246 – 266	21	Potential
	Transmembrane	280 – 300	21	Potential
	Transmembrane	308 – 328	21	Potential
	Transmembrane	336 – 356	21	Potential
	Transmembrane	380 – 400	21	Potential
	Transmembrane	402 – 422	21	Potential
	Transmembrane	468 – 488	21	Potential
Experimental info
	Sequence conflict	412	1	G → R in BAA14330. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P72714-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: C18C06704C277081	FASTA	521	55,355
	10 20 30 40 50 60 MDFSSNVAAQ LNAGTILPEG IVIVTLLLVL IVDLIGGRKV ALALPYLAIA GLLVSVGLLV 70 80 90 100 110 120 TSWSMADPIG FIGAFNGDNL SIIFRAIIAL STVVTILMSV RYVQQTGTSL AEFIAILLTA 130 140 150 160 170 180 TLGGMFLSAA NELVMVFISL EMLSISSYLM TGYMKRDPRS NEAALKYLLI GASSSAIFLY 190 200 210 220 230 240 GLSLLYGLSG GETQLVLIAE KLVNADTVGQ SLGLAIALVF VIAGIAFKIS AVPFHQWTPD 250 260 270 280 290 300 VYEGSPTPVV AFLSVGSKAA GFAVAIRLLV TAFGGITDEW HVIFTALAVL SMVLGNVVAL 310 320 330 340 350 360 AQTSMKRMLA YSSIGQAGFV MIGLVAGSED GYASMVFYML IYLFMNLGAF SCIILFTLRT 370 380 390 400 410 420 GSDQISDYAG LYHKDPLLTL GLSICLLSLG GIPPLAGFFG KIYIFWAGWQ SGLYGLVLLG 430 440 450 460 470 480 LVTSVVSIYY YIRVVKMMVV KEPQEMSEVI KNYPAIKWNL PGMRPLQVGI VATLVATSLA 490 500 510 520 GILANPLFNL ATDSVVSTKM LQTALQQTGE TPAIAISHDL P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene homologous to the subunit-2 gene of NADH dehydrogenase is essential to inorganic carbon transport of Synechocystis PCC6803." Ogawa T. Proc. Natl. Acad. Sci. U.S.A. 88:4275-4279(1991) [PubMed: 1903537] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D90288 Genomic DNA. Translation: BAA14330.1. BA000022 Genomic DNA. Translation: BAA16721.1.
PIR	S74569.
RefSeq	NP_440041.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P72714. 9 interactions.
STRING	P72714.
Genome annotation databases
GeneID	953340.
GenomeReviews	Gene locus sll0223 in contig BA000022_GR.
KEGG	syn:sll0223.
NMPDR	fig|1148.1.peg.142.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P72714.
OMA	ILAMKRN
Enzyme and pathway databases
BioCyc	SSP1148:SLL0223-MON.
Family and domain databases
HAMAP	MF_00445. [Tree]
InterPro	IPR010096. NADH-Q_OxRdtase_chainN. IPR001750. NADH_UbQ/plastoQ_OxRdtase. IPR003916. NADH_UbQ_OxRdtase_chain5. [Graphical view]
Pfam	PF00361. Oxidored_q1. 1 hit. [Graphical view]
PRINTS	PR01434. NADHDHGNASE5.
TIGRFAMs	TIGR01770. NDH_I_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NU2C_SYNY3

Accession

Primary (citable) accession number: P72714
Secondary accession number(s): Q55269

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 24, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)