P72640 (LEP4_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type 4 prepilin-like proteins leader peptide-processing enzyme

Including the following 2 domains:

1. Leader peptidase
   EC=3.4.23.43
   Alternative name(s):
   Prepilin peptidase
2. N-methyltransferase
   EC=2.1.1.-

Gene names

Name:	hofD
Ordered Locus Names:	slr1120

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue By similarity.
Catalytic activity	Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the peptidase A24 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Methyltransferase Protease Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: InterPro methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Type 4 prepilin-like proteins leader peptide-processing enzyme		PRO_0000192628
Regions
	Transmembrane	4 – 24	21	Helical; Potential
	Transmembrane	100 – 120	21	Helical; Potential
	Transmembrane	127 – 147	21	Helical; Potential
	Transmembrane	154 – 174	21	Helical; Potential
	Transmembrane	197 – 217	21	Helical; Potential
	Transmembrane	238 – 258	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P72640 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 4056E0AA96654B1C	FASTA	269	29,767
	10 20 30 40 50 60 MDPLIAPLAF LLAIALGCAV GSFLNVVAYR LPEGLSLVHP PSRCPHCGHR LGPKENVPVV 70 80 90 100 110 120 GWLWLRGKCR WCQTAISPRY PLVEAATGFL FALTCWRFGW QWQTFGYWIL ISFLISLTLI 130 140 150 160 170 180 DWDTMTLPNS LTKPGLVLGL LFHLLLGWQR GHWIVPLVEA IASAVLGLWL FDLIRMGGSL 190 200 210 220 230 240 LLGREGMGDG DPKLASMVGA WLGWPSLLLT TFIACFIGSI YGGLKLLLGT LQRRQGFPFG 250 260 PFLAIGALIS LFWGEKLITS YLNFVTPQF

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA16642.1.
PIR	S74490.
RefSeq	NP_439962.1. NC_000911.1. YP_005650017.1. NC_017277.1. YP_007449845.1. NC_020286.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	1148.slr1120.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA16642; BAA16642; BAA16642.
GeneID	12253770. 14615486. 952892.
KEGG	syn:slr1120. syy:SYNGTS_0064.
PATRIC	23836990. VBISynSp132158_0070.
Phylogenomic databases
eggNOG	COG1989.
HOGENOM	HOG000248583.
KO	K02654.
OMA	RCAYCHE.
Family and domain databases
InterPro	IPR010627. Pept_A24A_N. IPR014032. Peptidase_A24A_bac. IPR000045. Prepilin_IV_endopep_pep. [Graphical view]
Pfam	PF06750. DiS_P_DiS. 1 hit. PF01478. Peptidase_A24. 1 hit. [Graphical view]
PRINTS	PR00864. PREPILNPTASE.
ProtoNet	Search...

Entry information

Entry name

LEP4_SYNY3

Accession

Primary (citable) accession number: P72640

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program