Reviewed, UniProtKB/Swiss-Prot P72584 (ACSF1_SYNY3)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 1
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase 1
    EC=1.14.13.81

Gene names

Name:	acsF1
Ordered Locus Names:	sll1214

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + H(2)O. 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O. 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide + NADP(+) + 2 H(2)O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent).
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 1		PRO_0000217539

Sequences

	Sequence		Length	Mass (Da)
	P72584-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 6E8BD879AB4767DC	FASTA	358	42,153
	10 20 30 40 50 60 MVNTLEKPGF DEIRPGVKTP AKETILTPRF YTTDFDEMAK MDISPNEDEL RAILEEFRVD 70 80 90 100 110 120 YNRHHFVRNE SFNKSWDHID GEKRQLFVEF LERSCTAEFS GFLLYKELGR RLKNKNPLLA 130 140 150 160 170 180 ECFNLMSRDE ARHAGFLNKA MSDFNLSLDL GFLTKSRKYT FFKPKFIFYA TYLSEKIGYW 190 200 210 220 230 240 RYITIYRHLE KNPNDCIYPI FEFFENWCQD ENRHGDFFDA IMRAQPHTLN DWKAKLWCRF 250 260 270 280 290 300 FLLSVFATMY LNDTQRADFY ACLGLEARSY DKEVIEKTNE TAGRVFPIIL DVNNPEFYNR 310 320 330 340 350 LETCVSNNEQ LRAIDASGAP GVIKALRKLP IFASNGWQFI KLYLMKPIAV DQLAGAVR

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA16583.1.
PIR	S74431.
RefSeq	NP_439903.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P72584.
Genome annotation databases
GeneID	951877.
GenomeReviews	Gene locus sll1214 in contig BA000022_GR.
KEGG	syn:sll1214.
NMPDR	fig|1148.1.peg.4.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P72584.
Enzyme and pathway databases
BioCyc	SSP1148:SLL1214-MON.
Family and domain databases
HAMAP	MF_01840. [Tree]
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF1_SYNY3

Accession

Primary (citable) accession number: P72584

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	February 1, 1997
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)