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Protein

DNA topoisomerase 4 subunit A

Gene

parC

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA (PubMed:17375187, PubMed:20596531). Performs the decatenation events required during the replication of a circular DNA molecule.UniRule annotation2 Publications

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation2 Publications

Enzyme regulationi

Inhibited by quinolones, such as levofloxacin (PubMed:20596531).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei117Transition state stabilizer1
Active sitei118O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Topoisomerase IV subunit AUniRule annotation
Gene namesi
Name:parCUniRule annotation
Ordered Locus Names:SP_0855
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000585 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2363033.
DrugBankiDB06771. Besifloxacin.
DB01044. Gatifloxacin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001454181 – 823DNA topoisomerase 4 subunit AAdd BLAST823

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE (PubMed:17375187, PubMed:20596531).UniRule annotation2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei38Interaction with DNA1
Sitei74Interaction with DNA1
Sitei76Interaction with DNA1
Sitei87Interaction with DNA1
Sitei93Interaction with DNA1

Protein-protein interaction databases

DIPiDIP-48521N.
STRINGi170187.SP_0855.

Structurei

Secondary structure

1823
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Helixi9 – 26Combined sources18
Turni27 – 29Combined sources3
Turni33 – 35Combined sources3
Helixi39 – 50Combined sources12
Helixi62 – 72Combined sources11
Helixi78 – 88Combined sources11
Turni91 – 93Combined sources3
Beta strandi98 – 103Combined sources6
Turni116 – 118Combined sources3
Beta strandi120 – 123Combined sources4
Helixi125 – 130Combined sources6
Turni131 – 133Combined sources3
Helixi134 – 136Combined sources3
Beta strandi141 – 143Combined sources3
Beta strandi147 – 154Combined sources8
Helixi161 – 165Combined sources5
Beta strandi167 – 170Combined sources4
Beta strandi175 – 178Combined sources4
Helixi183 – 195Combined sources13
Beta strandi196 – 198Combined sources3
Helixi201 – 207Combined sources7
Beta strandi218 – 220Combined sources3
Helixi222 – 231Combined sources10
Beta strandi232 – 239Combined sources8
Beta strandi241 – 247Combined sources7
Turni248 – 250Combined sources3
Beta strandi251 – 258Combined sources8
Helixi265 – 277Combined sources13
Beta strandi280 – 283Combined sources4
Beta strandi286 – 289Combined sources4
Beta strandi298 – 301Combined sources4
Helixi308 – 318Combined sources11
Beta strandi322 – 328Combined sources7
Beta strandi330 – 333Combined sources4
Beta strandi336 – 339Combined sources4
Helixi342 – 383Combined sources42
Helixi385 – 394Combined sources10
Beta strandi395 – 397Combined sources3
Helixi398 – 409Combined sources12
Helixi413 – 420Combined sources8
Helixi424 – 428Combined sources5
Helixi432 – 454Combined sources23
Helixi456 – 474Combined sources19
Beta strandi480 – 482Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NOVX-ray2.67A/B/C/D1-488[»]
3FOEX-ray4.00A/B1-488[»]
3FOFX-ray4.00A/B1-488[»]
3K9FX-ray2.90A/B1-488[»]
3KSAX-ray3.30A/B1-488[»]
3KSBX-ray3.50A/B1-488[»]
3LTNX-ray3.10A/B1-488[»]
3RADX-ray3.35A/B1-488[»]
3RAEX-ray2.90A/B1-488[»]
3RAFX-ray3.24A/B1-488[»]
4JUOX-ray6.53A1-488[»]
4KOEX-ray3.02A/B1-488[»]
4KPEX-ray3.43A/B1-488[»]
4KPFX-ray3.24A/B1-488[»]
4Z3OX-ray3.44A/B3-484[»]
4Z4QX-ray3.04A/B3-484[»]
4Z53X-ray3.26A/B3-484[»]
ProteinModelPortaliP72525.
SMRiP72525.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP72525.

Family & Domainsi

Sequence similaritiesi

Belongs to the type II topoisomerase GyrA/ParC subunit family. ParC type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C24. Bacteria.
COG0188. LUCA.
HOGENOMiHOG000076279.
KOiK02621.
OMAiNVLIEMH.

Family and domain databases

Gene3Di3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPiMF_00937. ParC_type2. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR006691. GyrA/parC_pinwhl.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR005741. TopoIV_A_Gpos.
[Graphical view]
PfamiPF03989. DNA_gyraseA_C. 5 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTiSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01061. parC_Gpos. 1 hit.

Sequencei

Sequence statusi: Complete.

P72525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNIQNMSLE DIMGERFGRY SKYIIQDRAL PDIRDGLKPV QRRILYSMNK
60 70 80 90 100
DSNTFDKSYR KSAKSVGNIM GNFHPHGDSS IYDAMVRMSQ NWKNREILVE
110 120 130 140 150
MHGNNGSMDG DPPAAMRYTE ARLSEIAGYL LQDIEKKTVP FAWNFDDTEK
160 170 180 190 200
EPTVLPAAFP NLLVNGSTGI SAGYATDIPP HNLAEVIDAA VYMIDHPTAK
210 220 230 240 250
IDKLMEFLPG PDFPTGAIIQ GRDEIKKAYE TGKGRVVVRS KTEIEKLKGG
260 270 280 290 300
KEQIVIIEIP YEINKANLVK KIDDVRVNNK VAGIAEVRDE SDRDGLRIAI
310 320 330 340 350
ELKKDANTEL VLNYLFKYTD LQINYNFNMV AIDNFTPRQV GIVPILSSYI
360 370 380 390 400
AHRREVILAR SRFDKEKAEK RLHIVEGLIR VISILDEVIA LIRASENKAD
410 420 430 440 450
AKENLKVSYD FTEEQAEAIV TLQLYRLTNT DVVVLQEEEA ELREKIAMLA
460 470 480 490 500
AIIGDERTMY NLMKKELREV KKKFATPRLS SLEDTAKAIE IDTASLIAEE
510 520 530 540 550
DTYVSVTKAG YIKRTSPRSF AASTLEEIGK RDDDRLIFVQ SAKTTQHLLM
560 570 580 590 600
FTSLGNVIYR PIHELADIRW KDIGEHLSQT ITNFETNEEI LYVEVLDQFD
610 620 630 640 650
DATTYFAVTR LGQIKRVERK EFTPWRTYRS KSVKYAKLKD DTDQIVAVAP
660 670 680 690 700
IKLDDVVLVS QNGYALRFNI EEVPVVGAKA AGVKAMNLKE DDVLQSGFIC
710 720 730 740 750
NTSSFYLLTQ RGSLKRVSIE EILATSRAKR GLQVLRELKN KPHRVFLAGA
760 770 780 790 800
VAEQGFVGDF FSTEVDVNDQ TLLVQSNKGT IYESRLQDLN LSERTSNGSF
810 820
ISDTISDEEV FDAYLQEVVT EDK
Length:823
Mass (Da):93,133
Last modified:September 26, 2001 - v3
Checksum:iD4D17BD5DED63C05
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95R → C in AAB08038 (PubMed:8913454).Curated1
Sequence conflicti257I → T in CAA91551 (PubMed:8763932).Curated1
Sequence conflicti257I → T in CAA65021 (PubMed:8891124).Curated1
Sequence conflicti362R → P in CAA65021 (PubMed:8891124).Curated1
Sequence conflicti373H → R in CAA65021 (PubMed:8891124).Curated1
Sequence conflicti401A → P in CAA65021 (PubMed:8891124).Curated1
Sequence conflicti473K → N in CAA65021 (PubMed:8891124).Curated1
Sequence conflicti589E → A in CAA65021 (PubMed:8891124).Curated1
Sequence conflicti608V → A in CAA65021 (PubMed:8891124).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z67739 Genomic DNA. Translation: CAA91551.2.
AE005672 Genomic DNA. Translation: AAK74984.1.
X95717 Genomic DNA. Translation: CAA65021.1.
U49088 Genomic DNA. Translation: AAB08038.1.
PIRiG95098.

Genome annotation databases

EnsemblBacteriaiAAK74984; AAK74984; SP_0855.
KEGGispn:SP_0855.
PATRICi19706095. VBIStrPne105772_0894.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z67739 Genomic DNA. Translation: CAA91551.2.
AE005672 Genomic DNA. Translation: AAK74984.1.
X95717 Genomic DNA. Translation: CAA65021.1.
U49088 Genomic DNA. Translation: AAB08038.1.
PIRiG95098.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NOVX-ray2.67A/B/C/D1-488[»]
3FOEX-ray4.00A/B1-488[»]
3FOFX-ray4.00A/B1-488[»]
3K9FX-ray2.90A/B1-488[»]
3KSAX-ray3.30A/B1-488[»]
3KSBX-ray3.50A/B1-488[»]
3LTNX-ray3.10A/B1-488[»]
3RADX-ray3.35A/B1-488[»]
3RAEX-ray2.90A/B1-488[»]
3RAFX-ray3.24A/B1-488[»]
4JUOX-ray6.53A1-488[»]
4KOEX-ray3.02A/B1-488[»]
4KPEX-ray3.43A/B1-488[»]
4KPFX-ray3.24A/B1-488[»]
4Z3OX-ray3.44A/B3-484[»]
4Z4QX-ray3.04A/B3-484[»]
4Z53X-ray3.26A/B3-484[»]
ProteinModelPortaliP72525.
SMRiP72525.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48521N.
STRINGi170187.SP_0855.

Chemistry databases

ChEMBLiCHEMBL2363033.
DrugBankiDB06771. Besifloxacin.
DB01044. Gatifloxacin.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK74984; AAK74984; SP_0855.
KEGGispn:SP_0855.
PATRICi19706095. VBIStrPne105772_0894.

Phylogenomic databases

eggNOGiENOG4105C24. Bacteria.
COG0188. LUCA.
HOGENOMiHOG000076279.
KOiK02621.
OMAiNVLIEMH.

Miscellaneous databases

EvolutionaryTraceiP72525.
PROiP72525.

Family and domain databases

Gene3Di3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPiMF_00937. ParC_type2. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR006691. GyrA/parC_pinwhl.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR005741. TopoIV_A_Gpos.
[Graphical view]
PfamiPF03989. DNA_gyraseA_C. 5 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTiSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01061. parC_Gpos. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPARC_STRPN
AccessioniPrimary (citable) accession number: P72525
Secondary accession number(s): P72537, Q54917
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 26, 2001
Last modified: November 2, 2016
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.