Reviewed, UniProtKB/Swiss-Prot P72392 (FABH1_STRCO)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 1
    EC=2.3.1.180
Alternative name(s):
    3-oxoacyl-[acyl-carrier-protein] synthase III protein 1
    Beta-ketoacyl-ACP synthase III 1
      Short name=KAS III 1

Gene names

Name:	fabH1
Ordered Locus Names:	SCO2388
ORF Names:	SC4A7.16

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Essential enzyme that catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain of fatty acids. Ref.3
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacyl-[acyl-carrier-protein] + CoA + CO2. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/acpP and fabH By similarity.
Sequence similarities	Belongs to the fabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: HAMAP beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 1 HAMAP MF_01815		PRO_0000110483
Regions
	Region	269 – 273	5	ACP-binding By similarity
Sites
	Active site	122	1	By similarity
	Active site	268	1	By similarity
	Active site	299	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P72392-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 7838429F06A1AF21	FASTA	343	35,982
	10 20 30 40 50 60 MSKIKPSKGA PYARILGVGG YRPTRVVPNE VILEKIDSSD EWIRSRSGIE TRHWAGPEET 70 80 90 100 110 120 VAAMSVEASG KALADAGIDA SRIGAVVVST VSHFSQTPAI ATEIADRLGT DKAAAFDISA 130 140 150 160 170 180 GCAGFGYGLT LAKGMVVEGS AEYVLVIGVE RLSDLTDLED RATAFLFGDG AGAVVVGPSQ 190 200 210 220 230 240 EPAIGPTVWG SEGDKAETIK QTVSWDRFRI GDVSELPLDS EGNVKFPAIT QEGQAVFRWA 250 260 270 280 290 300 VFEMAKVAQQ ALDAAGISPD DLDVFIPHQA NVRIIDSMVK TLKLPEHVTV ARDIRTTGNT 310 320 330 340 SAASIPLAME RLLATGDARS GDTALVIGFG AGLVYAATVV TLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification of a malonyltransferase from Streptomyces coelicolor A3(2) and analysis of its genetic determinant." Revill W.P., Bibb M.J., Hopwood D.A. J. Bacteriol. 177:3946-3952(1995) [PubMed: 7608065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A3(2).
[2]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.
[3]	"Beta-ketoacyl acyl carrier protein synthase III (FabH) is essential for fatty acid biosynthesis in Streptomyces coelicolor A3(2)." Revill W.P., Bibb M.J., Scheu A.-K., Kieser H.J., Hopwood D.A. J. Bacteriol. 183:3526-3530(2001) [PubMed: 11344162] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X86475 Genomic DNA. Translation: CAA60200.1. AL939112 Genomic DNA. Translation: CAB62720.1.
RefSeq	NP_626634.1.
3D structure databases
HSSP	HSSP built from PDB template 1HZP based on UniProtKB O06399.
ModBase	Search...
Genome annotation databases
GeneID	1097822.
GenomeReviews	Gene locus SCO2388 in contig AL645882_GR.
KEGG	sco:SCO2388.
NMPDR	fig|100226.1.peg.2353.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P72392.
OMA	VPNEVIL.
Enzyme and pathway databases
BioCyc	SCOE100226:SCO2388-MON.
BRENDA	2.3.1.180. 1084.
Family and domain databases
HAMAP	MF_01815. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. [Graphical view]
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00747. fabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH1_STRCO

Accession

Primary (citable) accession number: P72392

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	February 1, 1997
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)