P72360 (SCDA_STAA8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Iron-sulfur cluster repair protein ScdA

Alternative name(s):
Cell wall-related protein ScdA

Gene names

Name:	scdA
Ordered Locus Names:	SAOUHSC_00229

Organism

Staphylococcus aureus (strain NCTC 8325) [Reference proteome] [HAMAP]

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Di-iron-containing protein involved in the repair of iron-sulfur clusters damaged by oxidative and nitrosative stress conditions. Ref.1 Ref.4
Subunit structure	Homodimer. Ref.4
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01156.
Induction	In stationary-phase. Induced by hydrogen peroxide. Ref.1 Ref.3 Ref.4
Disruption phenotype	Mutant shows increased sensitivity to oxidative stress. Ref.4
Sequence similarities	Belongs to the RIC family. ScdA subfamily.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein repair Inferred from mutant phenotype Ref.4. Source: UniProtKB response to oxidative stress Inferred from direct assay Ref.3. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Iron-sulfur cluster repair protein ScdA HAMAP-Rule MF_01156		PRO_0000220340
Experimental info
	Sequence conflict	43	1	E → G in AAB81287. Ref.1
	Sequence conflict	60 – 61	2	EQ → NK in AAB81287. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P72360 [UniParc]. Last modified July 11, 2006. Version 2. Checksum: EC8EBAE38DB70498	FASTA	224	25,485
	10 20 30 40 50 60 MINKNDIVAD VVTDYPKAAD IFRSVGIDFC CGGQVSIEAA ALEKKNVDLN ELLQRLNDVE 70 80 90 100 110 120 QTNTPGSLNP KFLNVSSLIQ YIQSAYHEPL REEFKNLTPY VTKLSKVHGP NHPYLVELKE 130 140 150 160 170 180 TYDTFKNGML EHMQKEDDVD FPKLIKYEQG EVVDDINTVI DDLVSDHIAT GELLVKMSEL 190 200 210 220 TSSYEPPIEA CGTWRLVYQR LKALEVLTHE HVHLENHVLF KKVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Staphylococcus aureus scdA gene: a novel locus that affects cell division and morphogenesis." Brunskill E.W., de Jonge B.L.M., Bayles K.W. Microbiology 143:2877-2882(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION. Strain: NCTC 8325.
[2]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 8325.
[3]	"Global transcriptome analysis of Staphylococcus aureus response to hydrogen peroxide." Chang W., Small D.A., Toghrol F., Bentley W.E. J. Bacteriol. 188:1648-1659(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: NCTC 8325.
[4]	"Widespread distribution in pathogenic bacteria of di-iron proteins that repair oxidative and nitrosative damage to iron-sulfur centers." Overton T.W., Justino M.C., Li Y., Baptista J.M., Melo A.M., Cole J.A., Saraiva L.M. J. Bacteriol. 190:2004-2013(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, INDUCTION, DISRUPTION PHENOTYPE, DI-IRON CENTER. Strain: NCTC 8325.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U57060 Genomic DNA. Translation: AAB81287.1. CP000253 Genomic DNA. Translation: ABD29404.1.
RefSeq	YP_498824.1. NC_007795.1.
3D structure databases
ProteinModelPortal	P72360.
SMR	P72360. Positions 2-64.
ModBase	Search...
Protein-protein interaction databases
STRING	93061.SAOUHSC_00229.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD29404; ABD29404; SAOUHSC_00229.
GeneID	3920304.
KEGG	sao:SAOUHSC_00229.
PATRIC	19578068. VBIStaAur99865_0210.
Phylogenomic databases
eggNOG	COG2846.
HOGENOM	HOG000286324.
KO	K07322.
OMA	GNPINAM.
ProtClustDB	PRK13276.
Enzyme and pathway databases
BioCyc	SAUR93061:GIWJ-218-MONOMER.
Family and domain databases
HAMAP	MF_01156. RIC_ScdA.
InterPro	IPR007500. DUF542_ScdA_N. IPR012312. Haemerythrin/HHE_cat-bd_motif. IPR019903. RIC_family. IPR023551. RIC_family_ScdA. [Graphical view]
Pfam	PF01814. Hemerythrin. 1 hit. PF04405. ScdA_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR03652. FeS_repair_RIC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SCDA_STAA8

Accession

Primary (citable) accession number: P72360
Secondary accession number(s): Q2G1B6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	July 11, 2006
Last modified:	May 1, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program