Reviewed, UniProtKB/Swiss-Prot P72324 (ADHI_RHOS4)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase class-3
    EC=1.1.1.1
Alternative name(s):
    Alcohol dehydrogenase class-III
    S-(hydroxymethyl)glutathione dehydrogenase
    EC=1.1.1.284
    Glutathione-dependent formaldehyde dehydrogenase
      Short name=GSH-FDH
      Short name=FALDH
      Short name=FDH
    EC=1.1.1.-

Gene names

Name:	adhI
Ordered Locus Names:	RHOS4_11650
ORF Names:	RSP_2576

Organism

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) [Complete proteome] [HAMAP]

Taxonomic identifier

272943 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidizes long-chain aliphatic alcohols, long-chain hydroxylated fatty acids and S-hydroxymethylglutathione (hmGSH) in increasing order of preference. Shows little or no activity with short-chain aliphatic alcohols.
Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH. S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Homodimer Probable.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ethanol oxidation Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	S-(hydroxymethyl)glutathione dehydrogenase activity Inferred from electronic annotation. Source: EC alcohol dehydrogenase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Alcohol dehydrogenase class-3		PRO_0000160780
Sites
	Metal binding	40	1	Zinc 1; catalytic By similarity
	Metal binding	62	1	Zinc 1; catalytic By similarity
	Metal binding	92	1	Zinc 2 By similarity
	Metal binding	95	1	Zinc 2 By similarity
	Metal binding	98	1	Zinc 2 By similarity
	Metal binding	106	1	Zinc 2 By similarity
	Metal binding	170	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P72324-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 9144690996B32BB9	FASTA	376	39,974
	10 20 30 40 50 60 MRTRAAVAVE AGKPLEIMEV NLEGPKAGEV MVEIKATGIC HTDEFTLSGA DPEGMFPAIL 70 80 90 100 110 120 GHEGAGVVVE VGPGVTSVKP GDHVIPLYTP ECRQCPSCLS QKTNLCTAIR GTQGQGLMPD 130 140 150 160 170 180 GTSRFSMLDG TPILHYMGCS TFSNYTVLPE IAVAKVRPDA PFDKICYIGC GVTTGIGAVI 190 200 210 220 230 240 NTAKVEIGAK AVVFGLGGIG LNVIQGLKLA GADMIIGVDL NNAKKEWGER FGMTHFVNPS 250 260 270 280 290 300 EIDGDVVAHL VNMTKTPFDQ IGGADYTFDC TGNVKVMRQA LEACHRGWGQ SIVIGVAPAG 310 320 330 340 350 360 AEIQTRPFQL VTGRVWKGSA FGGARGRTDV PKIVDWYMEG KIQIDPMITH ILSLEEINKG 370 FDLMHAGESI RSVVVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a glutathione-dependent formaldehyde dehydrogenase from Rhodobacter sphaeroides." Barber R.D., Rott M.A., Donohue T.J. J. Bacteriol. 178:1386-1393(1996) [PubMed: 8631716] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L47326 Genomic DNA. Translation: AAB09774.1. CP000143 Genomic DNA. Translation: ABA78733.1.
RefSeq	YP_352634.1.
3D structure databases
HSSP	HSSP built from PDB template 1M6H based on UniProtKB P11766.
ModBase	Search...
Genome annotation databases
GeneID	3720209.
GenomeReviews	Gene locus RHOS4_11650 in contig CP000143_GR.
KEGG	rsp:RSP_2576.
NMPDR	fig|272943.3.peg.1221.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P72324.
OMA	P72324. QKTNLCT.
Enzyme and pathway databases
BioCyc	RSPH272943:RSP_2576-MON.
Family and domain databases
InterPro	IPR014183. ADH_3. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02818. adh_III_F_hyde. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADHI_RHOS4

Accession

Primary (citable) accession number: P72324
Secondary accession number(s): Q3J3A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)