Reviewed, UniProtKB/Swiss-Prot P72292 (CHVG_RHIME)

Names and origin

Protein names

Recommended name:
    Sensor protein chvG
    EC=2.7.13.3
Alternative name(s):
    Histidine kinase sensory protein exoS

Gene names

Name:	chvG
Synonyms:	exoS
Ordered Locus Names:	R00043
ORF Names:	SMc04446

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Member of a two-component regulatory system chvG(exoS)/chvI involved in regulating the production of succinoglycan. Activates chvI by phosphorylation.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Pathway	Glycan metabolism; exopolysaccharide biosynthesis.
Subunit structure	Homodimer.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Exopolysaccharide synthesis Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro polysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 577	577	Sensor protein chvG		PRO_0000074721
Regions
	Topological domain	1 – 29	29	Cytoplasmic Potential
	Transmembrane	30 – 50	21	Potential
	Topological domain	51 – 260	210	Periplasmic Potential
	Transmembrane	261 – 281	21	Potential
	Topological domain	282 – 577	296	Cytoplasmic Potential
	Domain	283 – 339	57	HAMP
	Domain	347 – 575	229	Histidine kinase
Amino acid modifications
	Modified residue	350	1	Phosphohistidine By similarity
Experimental info
	Sequence conflict	136	1	L → F in AAB96631. Ref.1
	Sequence conflict	266	1	V → A in AAB96631. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P72292-1 [UniParc]. Last modified November 2, 2001. Version 3. Checksum: BEF2DD4A4F0E2B15	FASTA	577	63,721
	10 20 30 40 50 60 MRGQRRWAHP FTLIRRLFGN AVFSSLTRRI VFFNLVALVV LVGGIMYLNQ FREGLIDARV 70 80 90 100 110 120 ESLLTQGEII AGAISASASV DTNSITIDPE KLLELQAGES ITPLPSDEDL EFPIIQERVA 130 140 150 160 170 180 PVLRRLISPT RTRARLFDAD ADLLLDSRHL YSGGQVLRFD LPPVDPESPS LADEFGTWFN 190 200 210 220 230 240 RLLQPGDLPL YKEPPGGNGS IYPEVMNALT GVRGAVVRVT EKGELIVSVA VPVQRFRAVL 250 260 270 280 290 300 GVLLLSTQAG DIDKIVHAER LAIIRVFGVA ALVNVILSLL LSSTIANPLR RLSAAAIRVR 310 320 330 340 350 360 RGGAKEREEI PDFSSRQDEI GNLSVALREM TTALYDRIAA IENFAADVSH ELKNPLTSLR 370 380 390 400 410 420 SAVETLPLAR NEESKKRLMD VIQHDVRRLD RLISDISDAS RLDAELARAD AKKVDLEKLL 430 440 450 460 470 480 GDLVEISRQI RGSKKPVLLD FVVDRKDNPR ASFIVSGYEL RIGQIITNLI ENARSFVPEQ 490 500 510 520 530 540 NGRIVVRLTR SRLRCIVYVE DNGPGIQAED IDRIFERFYT DRPEGEDFGQ NSGLGLSISR 550 560 570 QIAEAHGGTL RAENIAGKDG RISGARFVLS LPAGPHP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Succinoglycan production by Rhizobium meliloti is regulated through the ExoS-ChvI two-component regulatory system." Cheng H.-P., Walker G.C. J. Bacteriol. 180:20-26(1998) [PubMed: 9422587] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1021.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[3]	"Identification of Rhizobium-specific intergenic mosaic elements within an essential two-component regulatory system of Rhizobium species." Oesteraas M., Stanley J., Finan T.M. J. Bacteriol. 177:5485-5494(1995) [PubMed: 7559334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-112. Strain: SU47 / 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF027298 Genomic DNA. Translation: AAB96631.1. AL591688 Genomic DNA. Translation: CAC41430.1. Different initiation. U32941 Genomic DNA. Translation: AAB07686.2.
RefSeq	NP_384149.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1231668.
GenomeReviews	Gene locus R00043 in contig AL591688_GR.
KEGG	sme:SMc04446.
NMPDR	fig|266834.1.peg.1337.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P72292.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC04446-MON.
BRENDA	2.7.13.3. 142.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR003660. HAMP_linker_domain. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHVG_RHIME

Accession

Primary (citable) accession number: P72292
Secondary accession number(s): O31069

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 2, 2001
Last modified:	June 16, 2009
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)