Reviewed, UniProtKB/Swiss-Prot P72242 (PEL_PSESL)

Names and origin

Protein names

Recommended name:
    Pectate lyase
      Short name=PL
    EC=4.2.2.2

Gene names

Name:

pelP

Organism

Pseudomonas syringae pv. lachrymans

Taxonomic identifier

53707 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas amygdali

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a role in bacterial invasion of plants.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Subcellular location	Secreted.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 379	350	Pectate lyase		PRO_0000024862
Sites
	Active site	264	1	Potential
	Metal binding	169	1	Calcium By similarity
	Metal binding	208	1	Calcium By similarity
	Metal binding	212	1	Calcium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P72242-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 22DBF8AB31949C92	FASTA	379	40,264
	10 20 30 40 50 60 MLKPHGLTPL ALTGGILVSL LSVSLSAHAE IATDVATTGW ATQNGGTKGG SRAAANNIYT 70 80 90 100 110 120 VKNAAELKAA LAASGGSNGR IIKERGVIDV SDGKPYTKTS DMKQRARLDI PGKTTIVGTS 130 140 150 160 170 180 SSAEIREGFF YAKENDVIIR NLTIENPWDP EPVWDPEDGS AGNWNSEYDG LTVEGASNVW 190 200 210 220 230 240 IDHVTFTDGR RTDDQNGTAN GRPKQHHDGA LDVKNGANYV TISYSVFRNH EKNNLIGSSD 250 260 270 280 290 300 SKTPDDGKLK VTNHNSLFEN ISSRGPRVRV GQVHLYNNHH IGSTTHKVYP CVYAQGVGKG 310 320 330 340 350 360 SKIFSERNVL DISGISGCSK VAADYGGSVY RDSGSLVNGS VISCSWSTSI GWTPPYSYTP 370 LAADKVAADV KAKAGAGKI

References

[1]	Bauer D.W., Collmer A. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 859.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U75414 Genomic DNA. Translation: AAB17879.1.
3D structure databases
SMR	P72242. Positions 36-379.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Enzyme and pathway databases
BRENDA	4.2.2.2. 297538.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
SMART	SM00656. Amb_all. 1 hit. SM00710. PbH1. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEL_PSESL

Accession

Primary (citable) accession number: P72242

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 1, 1997
Last modified:	January 19, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)