P72220 (BPHB_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase
EC=1.3.1.56

Alternative name(s):
2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase
2,3-dihydroxy-4-phenylhexa-4,6-diene dehydrogenase
Biphenyl-2,3-dihydro-2,3-diol dehydrogenase
Biphenyl-cis-diol dehydrogenase

Gene names

Name:

bphB

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Cis-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+ = biphenyl-2,3-diol + NADH.
Pathway	Xenobiotic degradation; biphenyl degradation; 2-hydroxy-2,4-pentadienoate and benzoate from biphenyl: step 2/4.
Subunit structure	Homotetramer Probable.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase		PRO_0000054535
Regions
	Nucleotide binding	9 – 33	25	NAD By similarity
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	142	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P72220 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: A7048159903BE527	FASTA	277	28,907
	10 20 30 40 50 60 MKLTGEVVLI TGGASGLGRA LVDRFVAERA KVAVLDKSAE RLAQLETDHG DNVLGVTGDV 70 80 90 100 110 120 RSLEDQKQAA SRCVAKFGKI DTLIPNAGIW DYSTALIDLP EESLDAAFDE VFHINVKGYI 130 140 150 160 170 180 HAVKACLPAL VASRGNVIFT ISNAGFYPNG GGPLYTAAKH AVVGLVRELA FELAPYVRVN 190 200 210 220 230 240 GVGPGGINSD LRGPSSLGMG GKAISTVPLA DMLKSVLPIG RMPEAEEYTG AYVFFATRGD 250 260 270 AAPATGALLN YDGGLGVRGF FSGAGGNDLL ERLNINS

References

[1]	"Nucleotide sequence of the gene encoding cis-biphenyl dihydrodiol dehydrogenase (bphB) and the expression of an active recombinant His-tagged bphB gene product from a PCB degrading bacterium, Pseudomonas putida OU83." Khan A.A., Wang R.F., Nawaz M.S., Cerniglia C.E. FEMS Microbiol. Lett. 154:317-324(1997) [PubMed: 9311131] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: OU83.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y07655 Genomic DNA. Translation: CAA68939.1.
3D structure databases
ProteinModelPortal	P72220.
SMR	P72220. Positions 1-276.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017711. BphB_TodD. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR03325. BphB_TodD. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BPHB_PSEPU

Accession

Primary (citable) accession number: P72220

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	February 1, 1997
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program