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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Proteus mirabilis
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.By similarity

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.

Pathway: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Lipid-A-disaccharide synthase (lpxB)
  6. no protein annotated in this organism
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00359; UER00481.

Protein family/group databases

CAZyiGT19. Glycosyltransferase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthase (EC:2.4.1.182)
Gene namesi
Name:lpxB
OrganismiProteus mirabilis
Taxonomic identifieri584 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProteus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›141›141Lipid-A-disaccharide synthasePRO_0000190175Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi529507.PMI2272.

Structurei

3D structure databases

ProteinModelPortaliP72216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.Curated

Family and domain databases

InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.

Sequencei

Sequence statusi: Fragment.

P72216-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLSGQSSIK QRPLVIGLVA GETSGDILGA GLIRALKQMH PNIRFVGVAG
60 70 80 90 100
PLMQAEGCEA WYEMEELAVM GIVEVLERLP RLLKIRKDLT QRFTQLKPDV
110 120 130 140
FVGIDAPDFN ITLEGRLKQK GLKTIHYVSP SVWAWRQKRV F
Length:141
Mass (Da):15,814
Last modified:February 1, 1997 - v1
Checksum:i551D19F25600561E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei141 – 1411

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09263 Genomic DNA. Translation: CAA70457.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09263 Genomic DNA. Translation: CAA70457.1.

3D structure databases

ProteinModelPortaliP72216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi529507.PMI2272.

Protein family/group databases

CAZyiGT19. Glycosyltransferase Family 19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00359; UER00481.

Family and domain databases

InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Servos S., Khan S.A., Papakonstantinopoulou A., Dougan G., Maskell D.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: PR 990.

Entry informationi

Entry nameiLPXB_PROMI
AccessioniPrimary (citable) accession number: P72216
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 24, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.