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Protein

Pyrolysin

Gene

pls

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has endopeptidase activity toward caseins, casein fragments including alpha-S1-casein and synthetic peptides.

Temperature dependencei

Thermostable. Highly active at 95 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei179Charge relay systemBy similarity1
Active sitei365Charge relay systemBy similarity1
Active sitei590Charge relay systemBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.B55. 5243.

Protein family/group databases

MEROPSiS08.100.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrolysin (EC:3.4.21.-)
Gene namesi
Name:pls
Ordered Locus Names:PF0287
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
PropeptideiPRO_000002712827 – 1491 PublicationAdd BLAST123
ChainiPRO_0000027129150 – 1398PyrolysinAdd BLAST1249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi152N-linked (GlcNAc...)1
Glycosylationi222N-linked (GlcNAc...)Sequence analysis1
Glycosylationi228N-linked (GlcNAc...)Sequence analysis1
Glycosylationi240N-linked (GlcNAc...)Sequence analysis1
Glycosylationi257N-linked (GlcNAc...)Sequence analysis1
Glycosylationi262N-linked (GlcNAc...)Sequence analysis1
Glycosylationi298N-linked (GlcNAc...)Sequence analysis1
Glycosylationi327N-linked (GlcNAc...)Sequence analysis1
Glycosylationi406N-linked (GlcNAc...)Sequence analysis1
Glycosylationi651N-linked (GlcNAc...)Sequence analysis1
Glycosylationi663N-linked (GlcNAc...)Sequence analysis1
Glycosylationi739N-linked (GlcNAc...)Sequence analysis1
Glycosylationi792N-linked (GlcNAc...)Sequence analysis1
Glycosylationi893N-linked (GlcNAc...)Sequence analysis1
Glycosylationi908N-linked (GlcNAc...)Sequence analysis1
Glycosylationi917N-linked (GlcNAc...)Sequence analysis1
Glycosylationi929N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1048N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1056N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1084N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1117N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1133N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1140N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1148N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1208N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1233N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1237N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1332N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

LWM pyrolysin seems to be produced by autoproteolytic activation of HMW pyrolysin.
Glycosylated.

Keywords - PTMi

Glycoprotein, Zymogen

Proteomic databases

PRIDEiP72186.

Interactioni

Protein-protein interaction databases

STRINGi186497.PF0287.

Structurei

3D structure databases

ProteinModelPortaliP72186.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini174 – 380Peptidase S8 1Add BLAST207
Domaini408 – 654Peptidase S8 2Add BLAST247

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 2 peptidase S8 domains.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiarCOG00702. Archaea.
arCOG03610. Archaea.
COG1404. LUCA.
OMAiKIVDWVT.

Family and domain databases

Gene3Di3.40.50.200. 2 hits.
InterProiIPR007280. Peptidase_C_arc/bac.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 5 hits.
PfamiPF00082. Peptidase_S8. 2 hits.
PF04151. PPC. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 4 hits.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P72186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKGLTVLF IAIMLLSVVP VHFVSAGTPP VSSENSTTSI LPNQQVVTKE
60 70 80 90 100
VSQAALNAIM KGQPNMVLII KTKEGKLEEA KTELEKLGAE ILDENRVLNM
110 120 130 140 150
LLVKIKPEKV KELNYISSLE KAWLNREVKL SPPIVEKDVK TKEPSLEPKM
160 170 180 190 200
YNSTWVINAL QFIQEFGYDG SGVVVAVLDT GVDPNHPFLS ITPDGRRKII
210 220 230 240 250
EWKDFTDEGF VDTSFSFSKV VNGTLIINTT FQVASGLTLN ESTGLMEYVV
260 270 280 290 300
KTVYVSNVTI GNITSANGIY HFGLLPERYF DLNFDGDQED FYPVLLVNST
310 320 330 340 350
GNGYDIAYVD TDLDYDFTDE VPLGQYNVTY DVAVFSYYYG PLNYVLAEID
360 370 380 390 400
PNGEYAVFGW DGHGHGTHVA GTVAGYDSNN DAWDWLSMYS GEWEVFSRLY
410 420 430 440 450
GWDYTNVTTD TVQGVAPGAQ IMAIRVLRSD GRGSMWDIIE GMTYAATHGA
460 470 480 490 500
DVISMSLGGN APYLDGTDPE SVAVDELTEK YGVVFVIAAG NEGPGINIVG
510 520 530 540 550
SPGVATKAIT VGAAAVPINV GVYVSQALGY PDYYGFYYFP AYTNVRIAFF
560 570 580 590 600
SSRGPRIDGE IKPNVVAPGY GIYSSLPMWI GGADFMSGTS MATPHVSGVV
610 620 630 640 650
ALLISGAKAE GIYYNPDIIK KVLESGATWL EGDPYTGQKY TELDQGHGLV
660 670 680 690 700
NVTKSWEILK AINGTTLPIV DHWADKSYSD FAEYLGVDVI RGLYARNSIP
710 720 730 740 750
DIVEWHIKYV GDTEYRTFEI YATEPWIKPF VSGSVILENN TEFVLRVKYD
760 770 780 790 800
VEGLEPGLYV GRIIIDDPTT PVIEDEILNT IVIPEKFTPE NNYTLTWYDI
810 820 830 840 850
NGPEMVTHHF FTVPEGVDVL YAMTTYWDYG LYRPDGMFVF PYQLDYLPAA
860 870 880 890 900
VSNPMPGNWE LVWTGFNFAP LYESGFLVRI YGVEITPSVW YINRTYLDTN
910 920 930 940 950
TEFSIEFNIT NIYAPINATL IPIGLGTYNA SVESVGDGEF FIKGIEVPEG
960 970 980 990 1000
TAELKIRIGN PSVPNSDLDL YLYDSKGNLV ALDGNPTAEE EVVVEYPKPG
1010 1020 1030 1040 1050
VYSIVVHGYS VRDENGNPTT TTFDLVVQMT LDNGNIKLDK DSIILGSNES
1060 1070 1080 1090 1100
VVVTANITID RDHPTGVYSG IIEIRDNEVY QDTNTSIAKI PITLVIDKAD
1110 1120 1130 1140 1150
FAVGLTPAEG VLGEARNYTL IVKHALTLEP VPNATVIIGN YTYLTDENGT
1160 1170 1180 1190 1200
VTFTYAPTKL GSDEITVIVK KENFNTLEKT FQITVSEPEI TEEDINEPKL
1210 1220 1230 1240 1250
AMSSPEANAT IVSVEMESEG GVKKTVTVEI TINGTANETA TIVVPVPKKA
1260 1270 1280 1290 1300
ENIEVSGDHV ISYSIEEGEY AKYVIITVKF ASPVTVTVTY TIYAGPRVSI
1310 1320 1330 1340 1350
LTLNFLGYSW YRLYSQKFDE LYQKALELGV DNETLALALS YHEKAKEYYE
1360 1370 1380 1390
KALELSEGNI IQYLGDIRLL PPLRQAYINE MKAVKILEKA IEELEGEE
Length:1,398
Mass (Da):154,475
Last modified:April 3, 2002 - v2
Checksum:i355D873A27D56552
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti607 – 609AKA → PKP in AAB09761 (PubMed:8702780).Curated3
Sequence conflicti881Y → H in AAB09761 (PubMed:8702780).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55835 Genomic DNA. Translation: AAB09761.1.
AE009950 Genomic DNA. Translation: AAL80411.1.
PIRiT28159.
RefSeqiWP_011011401.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80411; AAL80411; PF0287.
GeneIDi1468121.
KEGGipfu:PF0287.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55835 Genomic DNA. Translation: AAB09761.1.
AE009950 Genomic DNA. Translation: AAL80411.1.
PIRiT28159.
RefSeqiWP_011011401.1. NC_003413.1.

3D structure databases

ProteinModelPortaliP72186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0287.

Protein family/group databases

MEROPSiS08.100.

Proteomic databases

PRIDEiP72186.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80411; AAL80411; PF0287.
GeneIDi1468121.
KEGGipfu:PF0287.

Phylogenomic databases

eggNOGiarCOG00702. Archaea.
arCOG03610. Archaea.
COG1404. LUCA.
OMAiKIVDWVT.

Enzyme and pathway databases

BRENDAi3.4.21.B55. 5243.

Family and domain databases

Gene3Di3.40.50.200. 2 hits.
InterProiIPR007280. Peptidase_C_arc/bac.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 5 hits.
PfamiPF00082. Peptidase_S8. 2 hits.
PF04151. PPC. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 4 hits.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLS_PYRFU
AccessioniPrimary (citable) accession number: P72186
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: April 3, 2002
Last modified: November 2, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.