Reviewed, UniProtKB/Swiss-Prot P72173 (AAT_PSEAE)

Names and origin

Protein names

Recommended name:
    Aspartate aminotransferase
      Short name=AspAT
    EC=2.6.1.1
Alternative name(s):
    Transaminase A

Gene names

Name:	aspC
Ordered Locus Names:	PA3139

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Aspartate aminotransferase		PRO_0000123845
Amino acid modifications
	Modified residue	248	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P72173-1 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 4FE1D5709BAE599F	FASTA	398	43,319
	10 20 30 40 50 60 MSLFSAVEMA PRDPILGLNE AFNADTRPGK INLGVGVYYN EEGRIPLLRA VQAAEKARIE 70 80 90 100 110 120 AHAPRGYLPI EGIAAYDQGV QKLLFGNESE LLAAGRVVTT QAVGGTGALK LGADFLKRLL 130 140 150 160 170 180 PDATVAISDP SWENHRALFE AAGFPVQNYR YYDAASNGVN RAGLLEDLNA LPARSIVVLH 190 200 210 220 230 240 ACCHNPTGVD LELDDWKQVL DVLKAKGHVP FLDIAYQGFG NGIEEDAAAV RLFAQSGLSF 250 260 270 280 290 300 FVSSSFSKSF SLYGERVGAL SIVTESRDES ARVLSQVKRV IRTNYSNPPT HGASVVSSVL 310 320 330 340 350 360 NSPELRALWE QELGEMRDRI RDMRLAMVEQ LAAHGAKRDF SFVGRQRGMF SYSGLTADQV 370 380 390 ERLKTEFGIY AVSTGRICVA ALNKSNLETI TKAIVQVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"The uvrB gene of Pseudomonas aeruginosa is not DNA damage inducible." Rivera E., Vila L., Barbe J. J. Bacteriol. 178:5550-5554(1996) [PubMed: 8808952] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-75. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG06527.1. X93486 Genomic DNA. Translation: CAA63758.1.
PIR	B83252.
RefSeq	NP_251829.1.
3D structure databases
HSSP	HSSP built from PDB template 3TAT based on UniProtKB P04693.
ModBase	Search...
Genome annotation databases
GeneID	882671.
GenomeReviews	Gene locus PA3139 in contig AE004091_GR.
KEGG	pae:PA3139.
Organism-specific databases
PseudoCAP	PA3139.
CMR	Search...
Phylogenomic databases
HOGENOM	P72173.
OMA	VYFTDEG.
Enzyme and pathway databases
BioCyc	PAER208964:PA3139-MON.
BRENDA	2.6.1.1. 354.
Family and domain databases
InterPro	IPR004839. Aminotransferase_I/II. IPR000796. Asp_trans. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11879. Asp_trans. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00799. TRANSAMINASE.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAT_PSEAE

Accession

Primary (citable) accession number: P72173

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 8, 2000
Last modified:	November 3, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)