N5-carboxyaminoimidazole ribonucleotide synthase - Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

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P72158 (PURK_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 82. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthase
Short name=N5-CAIR synthase
EC=6.3.4.18

Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetase

Gene names

Name:	purK
Ordered Locus Names:	PA5425

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO₃^- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) By similarity.
Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO₃^- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	5-(carboxyamino)imidazole ribonucleotide synthase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: InterPro phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 360

360

N5-carboxyaminoimidazole ribonucleotide synthase

PRO_0000075002

Regions

Domain

102 – 285

184

ATP-grasp

Nucleotide binding

173 – 176

ATP By similarity

Nucleotide binding

255 – 256

ATP By similarity

Sites

Binding site

ATP By similarity

Binding site

138

ATP By similarity

Binding site

149

ATP By similarity

Binding site

181

ATP By similarity

Experimental info

Sequence conflict

124

L → V in AAB17258. Ref.1

Sequence conflict

133

L → V in AAB17258. Ref.1

Sequence conflict

196

F → L in AAB17258. Ref.1

Sequence conflict

217

G → A in AAB17258. Ref.1

Sequence conflict

353

E → D in AAB17258. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P72158 [UniParc].

Last modified December 8, 2000. Version 2.
Checksum: 88FBBEA510DE2B54
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FASTA

360

38,496

        10         20         30         40         50         60 
MKIGVIGGGQ LGRMLALAGT PLGMNFAFLD PAPDACAASL GEHIRADYGD QEHLRQLADE 

        70         80         90        100        110        120 
VDLVTFEFES VPAETVAFLS QFVPVYPNAE SLRIARDRWF EKSMFKDLGI PTPDFADVQS 

       130        140        150        160        170        180 
QADLDAAAAA IGLPAVLKTR TLGYDGKGQK VLRQPADVQG AFAELGSVPC ILEGFVPFTG 

       190        200        210        220        230        240 
EVSLVAVRAR DGETRFYPLV HNTHDSGILK LSVASSGHPL QALAEDYVGR VLARLDYVGV 

       250        260        270        280        290        300 
LAFEFFEVDG GLKANEIAPR VHNSGHWTIE GAECSQFENH LRAVAGLPLG STAKVGESAM 

       310        320        330        340        350        360 
LNFIGAVPPV AQVVAVADCH LHHYGKAFKN GRKVGHATLR CADRATLQAR IAEVEALIEA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Large-scale isolation of candidate virulence genes of Pseudomonas aeruginosa by in vivo selection." Wang J., Mushegian A., Lory S., Jin S. Proc. Natl. Acad. Sci. U.S.A. 93:10434-10439(1996) [PubMed: 8816818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PAK.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U58364 Genomic DNA. Translation: AAB17258.1. AE004091 Genomic DNA. Translation: AAG08810.1.
PIR	G82967.
RefSeq	NP_254112.1. NC_002516.2.
3D structure databases
ProteinModelPortal	P72158.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	878440.
GenomeReviews	Gene locus PA5425 in contig AE004091_GR.
KEGG	pae:PA5425.
NMPDR	fig\|208964.1.peg.5422.
PATRIC	19845727. VBIPseAer58763_5685.
Organism-specific databases
PseudoCAP	PA5425.
Phylogenomic databases
HOGENOM	HBG516369.
OMA	GRKMGHF.
ProtClustDB	PRK06019.
Enzyme and pathway databases
BioCyc	PAER208964:PA5425-MONOMER.
Family and domain databases
InterPro	IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01589.
Pfam	PF02222. ATP-grasp. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR01161. PurK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_PSEAE

Accession

Primary (citable) accession number: P72158

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 8, 2000
Last modified:	January 25, 2012
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents