Reviewed, UniProtKB/Swiss-Prot P72158 (PURK_PSEAE)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase ATPase subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purK
Ordered Locus Names:	PA5425

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses an ATPase activity that is dependent on the presence of AIR (aminoimidazole ribonucleotide). The association of purK and purE produces an enzyme complex capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Phosphoribosylaminoimidazole carboxylase ATPase subunit		PRO_0000075002
Regions
	Domain	102 – 285	184	ATP-grasp
	Nucleotide binding	173 – 176	4	ATP By similarity
	Nucleotide binding	255 – 256	2	ATP By similarity
Sites
	Binding site	98	1	ATP By similarity
	Binding site	138	1	ATP By similarity
	Binding site	149	1	ATP By similarity
	Binding site	181	1	ATP By similarity
Experimental info
	Sequence conflict	124	1	L → V in AAB17258. Ref.1
	Sequence conflict	133	1	L → V in AAB17258. Ref.1
	Sequence conflict	196	1	F → L in AAB17258. Ref.1
	Sequence conflict	217	1	G → A in AAB17258. Ref.1
	Sequence conflict	353	1	E → D in AAB17258. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P72158-1 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 88FBBEA510DE2B54	FASTA	360	38,496
	10 20 30 40 50 60 MKIGVIGGGQ LGRMLALAGT PLGMNFAFLD PAPDACAASL GEHIRADYGD QEHLRQLADE 70 80 90 100 110 120 VDLVTFEFES VPAETVAFLS QFVPVYPNAE SLRIARDRWF EKSMFKDLGI PTPDFADVQS 130 140 150 160 170 180 QADLDAAAAA IGLPAVLKTR TLGYDGKGQK VLRQPADVQG AFAELGSVPC ILEGFVPFTG 190 200 210 220 230 240 EVSLVAVRAR DGETRFYPLV HNTHDSGILK LSVASSGHPL QALAEDYVGR VLARLDYVGV 250 260 270 280 290 300 LAFEFFEVDG GLKANEIAPR VHNSGHWTIE GAECSQFENH LRAVAGLPLG STAKVGESAM 310 320 330 340 350 360 LNFIGAVPPV AQVVAVADCH LHHYGKAFKN GRKVGHATLR CADRATLQAR IAEVEALIEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Large-scale isolation of candidate virulence genes of Pseudomonas aeruginosa by in vivo selection." Wang J., Mushegian A., Lory S., Jin S. Proc. Natl. Acad. Sci. U.S.A. 93:10434-10439(1996) [PubMed: 8816818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PAK.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U58364 Genomic DNA. Translation: AAB17258.1. AE004091 Genomic DNA. Translation: AAG08810.1.
PIR	G82967.
RefSeq	NP_254112.1.
3D structure databases
HSSP	HSSP built from PDB template 1B6R based on UniProtKB P09029.
ModBase	Search...
Genome annotation databases
GeneID	878440.
GenomeReviews	Gene locus PA5425 in contig AE004091_GR.
KEGG	pae:PA5425.
NMPDR	fig|208964.1.peg.5422.
Organism-specific databases
PseudoCAP	PA5425.
CMR	Search...
Phylogenomic databases
HOGENOM	P72158.
OMA	RKIGHVN.
Enzyme and pathway databases
BioCyc	PAER208964:PA5425-MON.
BRENDA	4.1.1.21. 354.
Family and domain databases
InterPro	IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHER	PTHR23047:SF1. PurK_ATP. 1 hit.
Pfam	PF02222. ATP-grasp. 1 hit. [Graphical view]
TIGRFAMs	TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_PSEAE

Accession

Primary (citable) accession number: P72158

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 8, 2000
Last modified:	November 3, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)