Reviewed, UniProtKB/Swiss-Prot P72157 (PUR6_PSEAE)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase catalytic subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purE
Ordered Locus Names:	PA5426

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This subunit can alone transform AIR to CAIR, but in association with purK, which possesses an ATPase activity, an enzyme complex is produced which is capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the AIR carboxylase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 163	163	Phosphoribosylaminoimidazole carboxylase catalytic subunit		PRO_0000074978
Sites
	Binding site	11	1	Substrate By similarity
	Binding site	14	1	Substrate By similarity
	Binding site	41	1	Substrate By similarity
Experimental info
	Sequence conflict	46	1	L → F in AAB17257. Ref.1
	Sequence conflict	163	1	A → E in AAB17257. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P72157-1 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 447CA86962BC31FF	FASTA	163	16,889
	10 20 30 40 50 60 MSALVGVIMG SKSDWSTLSH TADMLDKLGI PYEVKVVSAH RTPDLLFQYA EEAEGRGLEV 70 80 90 100 110 120 IIAGAGGAAH LPGMCAAKTH LPVLGVPVQS SMLSGVDSLL SIVQMPAGVP VATLAIGKAG 130 140 150 160 AVNAALLSAS ILGAKHPQYH EALKQFRANQ TETVLDNPDP RDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Large-scale isolation of candidate virulence genes of Pseudomonas aeruginosa by in vivo selection." Wang J., Mushegian A., Lory S., Jin S. Proc. Natl. Acad. Sci. U.S.A. 93:10434-10439(1996) [PubMed: 8816818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PAK.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U58364 Genomic DNA. Translation: AAB17257.1. AE004091 Genomic DNA. Translation: AAG08811.1.
PIR	H82967.
RefSeq	NP_254113.1.
3D structure databases
HSSP	HSSP built from PDB template 1QCZ based on UniProtKB P09028.
SMR	P72157. Positions 1-147.
ModBase	Search...
Genome annotation databases
GeneID	877624.
GenomeReviews	Gene locus PA5426 in contig AE004091_GR.
KEGG	pae:PA5426.
Organism-specific databases
PseudoCAP	PA5426.
CMR	Search...
Phylogenomic databases
HOGENOM	P72157.
OMA	HPQFHAV.
Enzyme and pathway databases
BioCyc	PAER208964:PA5426-MON.
BRENDA	4.1.1.21. 354.
Family and domain databases
InterPro	IPR000031. AIR_COase_core. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit.
PANTHER	PTHR23046. AIR_carboxyl. 1 hit.
Pfam	PF00731. AIRC. 1 hit. [Graphical view]
ProDom	PD002193. AIR_carboxyl. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01162. purE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR6_PSEAE

Accession

Primary (citable) accession number: P72157

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 8, 2000
Last modified:	November 3, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)