Reviewed, UniProtKB/Swiss-Prot P72097 (LST_NEIMB)

Names and origin

Protein names

Recommended name:
    CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase
      Short name=Beta-galactoside alpha-2,3-sialyltransferase
      Short name=Alpha 2,3-ST
    EC=2.4.99.-
Alternative name(s):
    Lipooligosaccharide sialyltransferase

Gene names

Name:	lst
Ordered Locus Names:	NMB0922

Organism

Neisseria meningitidis serogroup B [Complete proteome] [HAMAP]

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transfers sialic acid from the substrate CMP-sialic acid donor to the terminal beta-D-galactosyl-1,4-acetyl-beta-D-glucosamine on the lacto-N-neotetraose branch of the lipooligosaccharide.
Catalytic activity	CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.
Pathway	Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Miscellaneous	Present in outer membrane vesicle formulations which are used as vaccines in human.
Sequence similarities	Belongs to the glycosyltransferase 52 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sialyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase		PRO_0000080574
Natural variations
	Natural variant	2	1	G → S in strain: M982B / NRCC 4725.
	Natural variant	29	1	Q → H in strain: 406Y / NRCC 4030 and NRCC 4725.
	Natural variant	40	1	E → D in strain: 406Y / NRCC 4030, M982B / NRCC 4725 and 126E / NRCC 4010.
	Natural variant	94	1	N → K in strain: 406Y / NRCC 4030 and M982B / NRCC 4725.
	Natural variant	102	1	R → W in strain: 126E / NRCC 4010.
	Natural variant	129	1	S → A in strain: 126E / NRCC 4010.
	Natural variant	168	1	G → I in strain: 126E / NRCC 4010.
	Natural variant	242	1	T → A in strain: 406Y / NRCC 4030 and 126E / NRCC 4010.
	Natural variant	273	1	K → N in strain: 406Y / NRCC 4030 and 126E / NRCC 4010.

Sequences

	Sequence		Length	Mass (Da)
	P72097-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 1C0E51DA3DAACD15	FASTA	371	42,611
	10 20 30 40 50 60 MGLKKACLTV LCLIVFCFGI FYTFDRVNQG ERNAVSLLKE KLFNEEGEPV NLIFCYTILQ 70 80 90 100 110 120 MKVAERIMAQ HPGERFYVVL MSENRNEKYD YYFNQIKDKA ERAYFFHLPY GLNKSFNFIP 130 140 150 160 170 180 TMAELKVKSM LLPKVKRIYL ASLEKVSIAA FLSTYPDAEI KTFDDGTGNL IQSSSYLGDE 190 200 210 220 230 240 FSVNGTIKRN FARMMIGDWS IAKTRNASDE HYTIFKGLKN IMDDGRRKMT YLPLFDASEL 250 260 270 280 290 300 KTGDETGGTV RILLGSPDKE MKEISEKAAK NFKIQYVAPH PRQTYGLSGV TTLNSPYVIE 310 320 330 340 350 360 DYILREIKKN PHTRYEIYTF FSGAALTMKD FPNVHVYALK PASLPEDYWL KPVYALFTQS 370 GIPILTFDDK N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the lipooligosaccharide alpha-2,3-sialyltransferase from the bacterial pathogens Neisseria meningitidis and Neisseria gonorrhoeae." Gilbert M., Watson D.C., Cunningham A.-M., Jennings M.P., Young N.M., Wakarchuk W.W. J. Biol. Chem. 271:28271-28276(1996) [PubMed: 8910446] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 406Y / NRCC 4030, M982B / NRCC 4725 and MC58 / NRCC 4728.
[2]	Gilbert M., Michniewicz J.J., Watson D.C., Wakarchuk W.W. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 126E / NRCC 4010.
[3]	"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.
[4]	"Characterization of the protein content of a meningococcal outer membrane vesicle vaccine by polyacrylamide gel electrophoresis and mass spectrometry." Vipond C., Wheeler J.X., Jones C., Feavers I.M., Suker J. Hum. Vaccin. 1:80-84(2005) [PubMed: 17038831] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[5]	"Proteomic analysis of a meningococcal outer membrane vesicle vaccine prepared from the group B strain NZ98/254." Vipond C., Suker J., Jones C., Tang C., Feavers I.M., Wheeler J.X. Proteomics 6:3400-3413(2006) [PubMed: 16645985] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U60660 Genomic DNA. Translation: AAC44541.1. U60661 Genomic DNA. Translation: AAC44543.1. U60662 Genomic DNA. Translation: AAC44544.2. U60663 Genomic DNA. Translation: AAC44545.1. AE002098 Genomic DNA. Translation: AAF41330.1.
PIR	D81143.
RefSeq	NP_273962.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT52. Glycosyltransferase Family 52.
Genome annotation databases
GeneID	903043.
GenomeReviews	Gene locus NMB0922 in contig AE002098_GR.
KEGG	nme:NMB0922.
NMPDR	fig|122586.1.peg.896.
TIGR	NMB0922.
Phylogenomic databases
HOGENOM	P72097.
OMA	P72097. ERIMAQH.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0922-MON.
Family and domain databases
InterPro	IPR012477. Glyco_transf_52. [Graphical view]
Pfam	PF07922. Glyco_transf_52. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LST_NEIMB

Accession

Primary (citable) accession number: P72097
Secondary accession number(s): P72099, P72100, P72101

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)