Reviewed,
UniProtKB/Swiss-Prot P72097 (LST_NEIMB)
Last modified
June 16, 2009.
Version 51.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase Short name=Beta-galactoside alpha-2,3-sialyltransferase Short name=Alpha 2,3-ST EC=2.4.99.- Alternative name(s): Lipooligosaccharide sialyltransferase | ||||
| Gene names |
| ||||
| Organism | Neisseria meningitidis serogroup B [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 491 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria |
Protein attributes
| Sequence length | 371 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Transfers sialic acid from the substrate CMP-sialic acid donor to the terminal beta-D-galactosyl-1,4-acetyl-beta-D-glucosamine on the lacto-N-neotetraose branch of the lipooligosaccharide. |
| Catalytic activity | CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine. |
| Pathway | Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. |
| Miscellaneous | Present in outer membrane vesicle formulations which are used as vaccines in human. |
| Sequence similarities | Belongs to the glycosyltransferase 52 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lipopolysaccharide biosynthesis |
| Molecular function | Glycosyltransferase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | sialyltransferase activity Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 371 | 371 | CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase | PRO_0000080574 | |||||
Natural variations | |||||||||
| Natural variant | 2 | 1 | G → S in strain: M982B / NRCC 4725. | ||||||
| Natural variant | 29 | 1 | Q → H in strain: 406Y / NRCC 4030 and NRCC 4725. | ||||||
| Natural variant | 40 | 1 | E → D in strain: 406Y / NRCC 4030, M982B / NRCC 4725 and 126E / NRCC 4010. | ||||||
| Natural variant | 94 | 1 | N → K in strain: 406Y / NRCC 4030 and M982B / NRCC 4725. | ||||||
| Natural variant | 102 | 1 | R → W in strain: 126E / NRCC 4010. | ||||||
| Natural variant | 129 | 1 | S → A in strain: 126E / NRCC 4010. | ||||||
| Natural variant | 168 | 1 | G → I in strain: 126E / NRCC 4010. | ||||||
| Natural variant | 242 | 1 | T → A in strain: 406Y / NRCC 4030 and 126E / NRCC 4010. | ||||||
| Natural variant | 273 | 1 | K → N in strain: 406Y / NRCC 4030 and 126E / NRCC 4010. | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning of the lipooligosaccharide alpha-2,3-sialyltransferase from the bacterial pathogens Neisseria meningitidis and Neisseria gonorrhoeae." Gilbert M., Watson D.C., Cunningham A.-M., Jennings M.P., Young N.M., Wakarchuk W.W. J. Biol. Chem. 271:28271-28276(1996) [PubMed: 8910446] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 406Y / NRCC 4030, M982B / NRCC 4725 and MC58 / NRCC 4728. |
| [2] | Gilbert M., Michniewicz J.J., Watson D.C., Wakarchuk W.W. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 126E / NRCC 4010. |
| [3] | "Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. Venter J.C.Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B. |
| [4] | "Characterization of the protein content of a meningococcal outer membrane vesicle vaccine by polyacrylamide gel electrophoresis and mass spectrometry." Vipond C., Wheeler J.X., Jones C., Feavers I.M., Suker J. Hum. Vaccin. 1:80-84(2005) [PubMed: 17038831] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [5] | "Proteomic analysis of a meningococcal outer membrane vesicle vaccine prepared from the group B strain NZ98/254." Vipond C., Suker J., Jones C., Tang C., Feavers I.M., Wheeler J.X. Proteomics 6:3400-3413(2006) [PubMed: 16645985] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
Cross-references
Sequence databases | |
|---|---|
| U60660 Genomic DNA. Translation: AAC44541.1. U60661 Genomic DNA. Translation: AAC44543.1. U60662 Genomic DNA. Translation: AAC44544.2. U60663 Genomic DNA. Translation: AAC44545.1. AE002098 Genomic DNA. Translation: AAF41330.1. | |
| PIR | D81143. |
| RefSeq | NP_273962.1. |
3D structure databases | |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GT52. Glycosyltransferase Family 52. |
Genome annotation databases | |
| GeneID | 903043. |
| GenomeReviews | Gene locus NMB0922 in contig AE002098_GR. |
| KEGG | nme:NMB0922. |
| NMPDR | fig|122586.1.peg.896. |
| TIGR | NMB0922. |
Phylogenomic databases | |
| HOGENOM | P72097. |
| OMA | P72097. ERIMAQH. |
Enzyme and pathway databases | |
| BioCyc | NMEN122586:NMB_0922-MON. |
Family and domain databases | |
| InterPro | IPR012477. Glyco_transf_52. [Graphical view] |
| Pfam | PF07922. Glyco_transf_52. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | LST_NEIMB | ||||||||
| Accession | Primary (citable) accession number: P72097 Secondary accession number(s): P72099, P72100, P72101 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


