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P72065

- GYRA_MYCXE

UniProt

P72065 - GYRA_MYCXE

Protein

DNA gyrase subunit A

Gene

gyrA

Organism
Mycobacterium xenopi
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 2 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.By similarity

    Catalytic activityi

    ATP-dependent breakage, passage and rejoining of double-stranded DNA.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA topological change Source: InterPro
    2. intein-mediated protein splicing Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Protein family/group databases

    MEROPSiN10.008.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA gyrase subunit A (EC:5.99.1.3)
    Cleaved into the following chain:
    Gene namesi
    Name:gyrA
    OrganismiMycobacterium xenopi
    Taxonomic identifieri1789 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 65›65DNA gyrase subunit A, 1st partPRO_0000034813Add
    BLAST
    Chaini66 – 263198Mxe gyrA inteinPRO_0000034814Add
    BLAST
    Chaini264 – ›327›64DNA gyrase subunit A, 2nd partPRO_0000034815Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Subunit structurei

    Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity.By similarity

    Structurei

    Secondary structure

    1
    327
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi72 – 743
    Beta strandi80 – 823
    Helixi83 – 853
    Beta strandi94 – 10310
    Beta strandi109 – 12820
    Beta strandi133 – 1364
    Beta strandi141 – 1488
    Beta strandi151 – 1588
    Helixi159 – 1613
    Beta strandi167 – 1715
    Helixi172 – 1743
    Helixi201 – 2077
    Beta strandi209 – 2113
    Helixi214 – 2229
    Beta strandi226 – 25126
    Beta strandi253 – 2564
    Beta strandi259 – 2624

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AM2X-ray2.20A67-263[»]
    4OZ6X-ray2.79A66-267[»]
    B62-65[»]
    ProteinModelPortaliP72065.
    SMRiP72065. Positions 3-65, 67-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP72065.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di2.170.16.10. 1 hit.
    3.90.199.10. 2 hits.
    InterProiIPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR006141. Intein_splice_site.
    IPR002205. Topo_IIA_A/C.
    IPR013758. Topo_IIA_A/C_ab.
    IPR013760. Topo_IIA_like_dom.
    [Graphical view]
    PfamiPF00521. DNA_topoisoIV. 2 hits.
    [Graphical view]
    SMARTiSM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    SM00434. TOP4c. 1 hit.
    [Graphical view]
    SUPFAMiSSF51294. SSF51294. 1 hit.
    SSF56719. SSF56719. 2 hits.
    TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    PROSITEiPS50818. INTEIN_C_TER. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P72065-1 [UniParc]FASTAAdd to Basket

    « Hide

    RPDRSHAKSA RSVAETMGNY HPHGDASIYD TLVRMAQPWS MRYPLVDGQG    50
    NFGSPGNDPP AAMRYCITGD ALVALPEGES VRIADIVPGA RPNSDNAIDL 100
    KVLDRHGNPV LADRLFHSGE HPVYTVRTVE GLRVTGTANH PLLCLVDVAG 150
    VPTLLWKLID EIKPGDYAVI QRSAFSVDCA GFARGKPEFA PTTYTVGVPG 200
    LVRFLEAHHR DPDAQAIADE LTDGRFYYAK VASVTDAGVQ PVYSLRVDTA 250
    DHAFITNGFV SHNTEAPLTP LAMEMLREID EETVDFIPNY DGRVQEPTVL 300
    PSRFPNLLAN GSGGIAVGMA TNIPPHN 327
    Length:327
    Mass (Da):35,386
    Last modified:January 1, 1998 - v2
    Checksum:iEFAE9924D97068FB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Non-terminal residuei327 – 3271

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U67876 Genomic DNA. Translation: AAB81424.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U67876 Genomic DNA. Translation: AAB81424.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AM2 X-ray 2.20 A 67-263 [» ]
    4OZ6 X-ray 2.79 A 66-267 [» ]
    B 62-65 [» ]
    ProteinModelPortali P72065.
    SMRi P72065. Positions 3-65, 67-327.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi N10.008.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P72065.

    Family and domain databases

    Gene3Di 2.170.16.10. 1 hit.
    3.90.199.10. 2 hits.
    InterProi IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR006141. Intein_splice_site.
    IPR002205. Topo_IIA_A/C.
    IPR013758. Topo_IIA_A/C_ab.
    IPR013760. Topo_IIA_like_dom.
    [Graphical view ]
    Pfami PF00521. DNA_topoisoIV. 2 hits.
    [Graphical view ]
    SMARTi SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    SM00434. TOP4c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51294. SSF51294. 1 hit.
    SSF56719. SSF56719. 2 hits.
    TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    PROSITEi PS50818. INTEIN_C_TER. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein."
      Telenti A., Southworth M., Alcaide F., Daugelat S., Jacobs W.R. Jr., Perler F.B.
      J. Bacteriol. 179:6378-6382(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: IMM 5024.
    2. "Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing."
      Klabunde T., Sharma S., Telenti A., Jacobs W.R. Jr., Sacchettini J.C.
      Nat. Struct. Biol. 5:31-36(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF INTEIN.

    Entry informationi

    Entry nameiGYRA_MYCXE
    AccessioniPrimary (citable) accession number: P72065
    Secondary accession number(s): P95323
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 93 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3