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P72065 (GYRA_MYCXE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA gyrase subunit A

EC=5.99.1.3

Cleaved into the following chain:

  1. Mxe gyrA intein
Gene names
Name:gyrA
OrganismMycobacterium xenopi
Taxonomic identifier1789 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length327 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity.

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA.

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity.

Subcellular location

Cytoplasm Potential.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Sequence similarities

Belongs to the topoisomerase GyrA/ParC subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 65›65DNA gyrase subunit A, 1st part
PRO_0000034813
Chain66 – 263198Mxe gyrA intein
PRO_0000034814
Chain264 – ›327›64DNA gyrase subunit A, 2nd part
PRO_0000034815

Sites

Active site651O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Experimental info

Non-terminal residue11
Non-terminal residue3271

Secondary structure

................................ 327
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P72065 [UniParc].

Last modified January 1, 1998. Version 2.
Checksum: EFAE9924D97068FB

FASTA32735,386
        10         20         30         40         50         60 
RPDRSHAKSA RSVAETMGNY HPHGDASIYD TLVRMAQPWS MRYPLVDGQG NFGSPGNDPP 

        70         80         90        100        110        120 
AAMRYCITGD ALVALPEGES VRIADIVPGA RPNSDNAIDL KVLDRHGNPV LADRLFHSGE 

       130        140        150        160        170        180 
HPVYTVRTVE GLRVTGTANH PLLCLVDVAG VPTLLWKLID EIKPGDYAVI QRSAFSVDCA 

       190        200        210        220        230        240 
GFARGKPEFA PTTYTVGVPG LVRFLEAHHR DPDAQAIADE LTDGRFYYAK VASVTDAGVQ 

       250        260        270        280        290        300 
PVYSLRVDTA DHAFITNGFV SHNTEAPLTP LAMEMLREID EETVDFIPNY DGRVQEPTVL 

       310        320 
PSRFPNLLAN GSGGIAVGMA TNIPPHN 

« Hide

References

[1]"The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein."
Telenti A., Southworth M., Alcaide F., Daugelat S., Jacobs W.R. Jr., Perler F.B.
J. Bacteriol. 179:6378-6382(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: IMM 5024.
[2]"Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing."
Klabunde T., Sharma S., Telenti A., Jacobs W.R. Jr., Sacchettini J.C.
Nat. Struct. Biol. 5:31-36(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF INTEIN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U67876 Genomic DNA. Translation: AAB81424.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AM2X-ray2.20A67-263[»]
4OZ6X-ray2.79A66-267[»]
B62-65[»]
ProteinModelPortalP72065.
SMRP72065. Positions 3-65, 67-327.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSN10.008.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.170.16.10. 1 hit.
3.90.199.10. 2 hits.
InterProIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006141. Intein_splice_site.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
[Graphical view]
PfamPF00521. DNA_topoisoIV. 2 hits.
[Graphical view]
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
SM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMSSF51294. SSF51294. 1 hit.
SSF56719. SSF56719. 2 hits.
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP72065.

Entry information

Entry nameGYRA_MYCXE
AccessionPrimary (citable) accession number: P72065
Secondary accession number(s): P95323
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Intein-containing proteins

List of intein-containing protein entries