Probable arabinosyltransferase C - Mycobacterium tuberculosis

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P72059 (EMBC_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable arabinosyltransferase C
EC=2.4.2.-

Gene names

Name:	embC
Ordered Locus Names:	Rv3793, MT3900
ORF Names:	MTCY13D12.27

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	1094 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Miscellaneous	This is one of the targets of the anti-tuberculosis drug ethambutol [(S,S')-2,2'-(ethylenediimino)di-1-butanol; EMB]. EMB is a first-line drug used to treat tuberculosis. EMB inhibits the transfer of arabinogalactan into the cell wall. Was identified as a high-confidence drug target.
Sequence similarities	Belongs to the emb family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW glycolipid biosynthetic process Inferred from mutant phenotype. Source: MTBBASE growth Inferred from mutant phenotype. Source: MTBBASE peptidoglycan biosynthetic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: MTBBASE integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arabinosyltransferase activity Inferred from mutant phenotype. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1094

1094

Probable arabinosyltransferase C

PRO_0000220573

Regions

Transmembrane

28 – 50

Helical; Potential

Transmembrane

232 – 251

Helical; Potential

Transmembrane

264 – 286

Helical; Potential

Transmembrane

341 – 360

Helical; Potential

Transmembrane

373 – 392

Helical; Potential

Transmembrane

431 – 453

Helical; Potential

Transmembrane

466 – 488

Helical; Potential

Transmembrane

530 – 552

Helical; Potential

Transmembrane

565 – 582

Helical; Potential

Transmembrane

586 – 608

Helical; Potential

Transmembrane

620 – 642

Helical; Potential

Transmembrane

657 – 679

Helical; Potential

Transmembrane

700 – 722

Helical; Potential

Natural variations

Natural variant

394

N → D Resistance to EMB. Ref.5

Natural variant

738

R → Q Resistance to EMB. Ref.5

Experimental info

Sequence conflict

981

V → L in AAK48266. Ref.3

Secondary structure

1094

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P72059 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: AF8190BE0A1EA05E
Show »

FASTA

1,094

117,490

        10         20         30         40         50         60 
MATEAAPPRI AVRLPSTSVR DAGANYRIAR YVAVVAGLLG AVLAIATPLL PVNQTTAQLN 

        70         80         90        100        110        120 
WPQNGTFASV EAPLIGYVAT DLNITVPCQA AAGLAGSQNT GKTVLLSTVP KQAPKAVDRG 

       130        140        150        160        170        180 
LLLQRANDDL VLVVRNVPLV TAPLSQVLGP TCQRLTFTAH ADRVAAEFVG LVQGPNAEHP 

       190        200        210        220        230        240 
GAPLRGERSG YDFRPQIVGV FTDLAGPAPP GLSFSASVDT RYSSSPTPLK MAAMILGVAL 

       250        260        270        280        290        300 
TGAALVALHI LDTADGMRHR RFLPARWWST GGLDTLVIAV LVWWHFVGAN TSDDGYILTM 

       310        320        330        340        350        360 
ARVSEHAGYM ANYYRWFGTP EAPFGWYYDL LALWAHVSTA SIWMRLPTLA MALTCWWVIS 

       370        380        390        400        410        420 
REVIPRLGHA VKTSRAAAWT AAGMFLAVWL PLDNGLRPEP IIALGILLTW CSVERAVATS 

       430        440        450        460        470        480 
RLLPVAIACI IGALTLFSGP TGIASIGALL VAIGPLRTIL HRRSRRFGVL PLVAPILAAA 

       490        500        510        520        530        540 
TVTAIPIFRD QTFAGEIQAN LLKRAVGPSL KWFDEHIRYE RLFMASPDGS IARRFAVLAL 

       550        560        570        580        590        600 
VLALAVSVAM SLRKGRIPGT AAGPSRRIIG ITIISFLAMM FTPTKWTHHF GVFAGLAGSL 

       610        620        630        640        650        660 
GALAAVAVTG AAMRSRRNRT VFAAVVVFVL ALSFASVNGW WYVSNFGVPW SNSFPKWRWS 

       670        680        690        700        710        720 
LTTALLELTV LVLLLAAWFH FVANGDGRRT ARPTRFRARL AGIVQSPLAI ATWLLVLFEV 

       730        740        750        760        770        780 
VSLTQAMISQ YPAWSVGRSN LQALAGKTCG LAEDVLVELD PNAGMLAPVT APLADALGAG 

       790        800        810        820        830        840 
LSEAFTPNGI PADVTADPVM ERPGDRSFLN DDGLITGSEP GTEGGTTAAP GINGSRARLP 

       850        860        870        880        890        900 
YNLDPARTPV LGSWRAGVQV PAMLRSGWYR LPTNEQRDRA PLLVVTAAGR FDSREVRLQW 

       910        920        930        940        950        960 
ATDEQAAAGH HGGSMEFADV GAAPAWRNLR APLSAIPSTA TQVRLVADDQ DLAPQHWIAL 

       970        980        990       1000       1010       1020 
TPPRIPRVRT LQNVVGAADP VFLDWLVGLA FPCQRPFGHQ YGVDETPKWR ILPDRFGAEA 

      1030       1040       1050       1060       1070       1080 
NSPVMDHNGG GPLGITELLM RATTVASYLK DDWFRDWGAL QRLTPYYPDA QPADLNLGTV 

      1090 
TRSGLWSPAP LRRG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol." Telenti A., Philipp W.J., Sreevatsan S., Bernasconi C., Stockbauer K.E., Wieles B., Musser J.M., Jacobs W.R. Jr. Nat. Med. 3:567-570(1997) [PubMed: 9142129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[4]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
[5]	"Molecular genetic analysis of nucleotide polymorphisms associated with ethambutol resistance in human isolates of Mycobacterium tuberculosis." Ramaswamy S.V., Amin A.G., Goeksel S., Stager C.E., Dou S.-J., El Sahly H., Moghazeh S.L., Kreiswirth B.N., Musser J.M. Antimicrob. Agents Chemother. 44:326-336(2000) [PubMed: 10639358] [Abstract] Cited for: VARIANTS EMB RESISTANT ASP-394 AND GLN-738.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BX842584 Genomic DNA. Translation: CAB02472.1.
U68480 Genomic DNA. Translation: AAC45279.1.
AE000516 Genomic DNA. Translation: AAK48266.1.

PIR

E70697.

RefSeq

NP_218310.1. NC_000962.2.
NP_338452.1. NC_002755.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3PTY	X-ray	2.00	A	719-1094	[»]

ProteinModelPortal

P72059.

ModBase

Search...

Protein family/group databases

CAZy

GT53. Glycosyltransferase Family 53.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBMYCT00000003078; EBMYCP00000003078; EBMYCG00000003076.
EBMYCT00000069959; EBMYCP00000068018; EBMYCG00000069954.

GeneID

886112.
922602.

GenomeReviews

Gene locus MT3900 in contig AE000516_GR.
Gene locus Rv3793 in contig AL123456_GR.

KEGG

mtc:MT3900.
mtu:Rv3793.

PATRIC

18130337. VBIMycTub22151_4256.

TIGR

MT3900.

Organism-specific databases

TubercuList

Rv3793.

Phylogenomic databases

GeneTree

EBGT00070000032055.

HOGENOM

HBG429120.

OMA

FGAPYYD.

ProtClustDB

CLSK2748678.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-15271.

Family and domain databases

InterPro

IPR007680. Mycobac_arabinan_synth.
[Graphical view]

K11387.

Pfam

PF04602. Arabinose_trans. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00330. Ethambutol.

Entry information

Entry name

EMBC_MYCTU

Accession

Primary (citable) accession number: P72059
Secondary accession number(s): O08116

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents