P72058 (AFTA_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arabinofuranosyltransferase AftA
EC=2.4.2.-

Gene names

Name:	aftA
Ordered Locus Names:	Rv3792, MT3899.1

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the biosynthesis of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component the mycobacterial cell wall. Catalyzes the addition of the first key arabinofuranosyl (Araf) residue from the sugar donor beta-D-arabinofuranosyl-1-monophosphoryldecaprenol (DPA) on the C-5 of a 6-linked galactofuranosyl (Galf) of the galactan domain, thus 'priming' the galactan for further elaboration by other arabinofuranosyltransferases. It is not able to add an Araf residue to a terminal Galf. Ref.3
Enzyme regulation	Not inhibited by the anti-tuberculosis drug ethambutol [(S,S')-2,2'-(ethylenediimino)di-1-butanol; EMB]. Ref.3
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.3.
Sequence similarities	Belongs to the glycosyltransferase 85 family.
Sequence caution	The sequence AAK48265.1 differs from that shown. Reason: Frameshift at position 614.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell wall macromolecule biosynthetic process Inferred from direct assay Ref.3. Source: UniProtKB growth Inferred from mutant phenotype. Source: MTBBASE mycolate cell wall layer assembly Inferred from mutant phenotype Ref.3. Source: MTBBASE response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	UDP-galactosyltransferase activity Inferred from direct assay. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 643	643	Arabinofuranosyltransferase AftA		PRO_0000250356
Regions
	Transmembrane	25 – 45	21	Helical; Potential
	Transmembrane	66 – 86	21	Helical; Potential
	Transmembrane	97 – 117	21	Helical; Potential
	Transmembrane	180 – 200	21	Helical; Potential
	Transmembrane	205 – 225	21	Helical; Potential
	Transmembrane	229 – 249	21	Helical; Potential
	Transmembrane	255 – 272	18	Helical; Potential
	Transmembrane	276 – 293	18	Helical; Potential
	Transmembrane	309 – 329	21	Helical; Potential
	Transmembrane	355 – 375	21	Helical; Potential
	Transmembrane	384 – 404	21	Helical; Potential
	Transmembrane	420 – 440	21	Helical; Potential
	Transmembrane	445 – 465	21	Helical; Potential
	Topological domain	466 – 643	178	Extracellular Potential

Sequences

	Sequence		Length	Mass (Da)
	P72058 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 7C58972B085D9EDA	FASTA	643	69,515
	10 20 30 40 50 60 MPSRRKSPQF GHEMGAFTSA RAREVLVALG QLAAAVVVAV GVAVVSLLAI ARVEWPAFPS 70 80 90 100 110 120 SNQLHALTTV GQVGCLAGLV GIGWLWRHGR FRRLARLGGL VLVSAFTVVT LGMPLGATKL 130 140 150 160 170 180 YLFGISVDQQ FRTEYLTRLT DTAALRDMTY IGLPPFYPPG WFWIGGRAAA LTGTPAWEMF 190 200 210 220 230 240 KPWAITSMAI AVAVALVLWW RMIRFEYALL VTVATAAVML AYSSPEPYAA MITVLLPPML 250 260 270 280 290 300 VLTWSGLGAR DRQGWAAVVG AGVFLGFAAT WYTLLVAYGA FTVVLMALLL AGSRLQSGIK 310 320 330 340 350 360 AAVDPLCRLA VVGAIAAAIG STTWLPYLLR AARDPVSDTG SAQHYLPADG AALTFPMLQF 370 380 390 400 410 420 SLLGAICLLG TLWLVMRARS SAPAGALAIG VLAVYLWSLL SMLATLARTT LLSFRLQPTL 430 440 450 460 470 480 SVLLVAAGAF GFVEAVQALG KRGRGVIPMA AAIGLAGAIA FSQDIPDVLR PDLTIAYTDT 490 500 510 520 530 540 DGYGQRGDRR PPGSEKYYPA IDAAIRRVTG KRRDRTVVLT ADYSFLSYYP YWGFQGLTPH 550 560 570 580 590 600 YANPLAQFDK RATQIDSWSG LSTADEFIAA LDKLPWQPPT VFLMRHGAHN SYTLRLAQDV 610 620 630 640 YPNQPNVRRY TVDLRTALFA DPRFVVEDIG PFVLAIRKPQ ESA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis." Alderwick L.J., Seidel M., Sahm H., Besra G.S., Eggeling L. J. Biol. Chem. 281:15653-15661(2006) [PubMed: 16595677] [Abstract] Cited for: FUNCTION AS A ARABINOFURANOSYLTRANSFERASE, ENZYME REGULATION, SUBCELLULAR LOCATION, NOMENCLATURE. Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842584 Genomic DNA. Translation: CAB02471.1. AE000516 Genomic DNA. Translation: AAK48265.1. Frameshift.
PIR	D70697.
RefSeq	NP_218309.1. NC_000962.2. NP_338451.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P72058.
ModBase	Search...
Protein family/group databases
CAZy	GT85. Glycosyltransferase Family 85.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000002455; EBMYCP00000002455; EBMYCG00000002453. EBMYCT00000070637; EBMYCP00000068696; EBMYCG00000070632.
GeneID	886127. 922612.
GenomeReviews	Gene locus MT3899.1 in contig AE000516_GR. Gene locus Rv3792 in contig AL123456_GR.
KEGG	mtc:MT3899.1. mtu:Rv3792.
PATRIC	18130335. VBIMycTub22151_4255.
TIGR	MT3899.1.
Organism-specific databases
TubercuList	Rv3792.
Phylogenomic databases
GeneTree	EBGT00070000032387.
HOGENOM	HBG429582.
OMA	AEDVYPN.
ProtClustDB	CLSK792726.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-15283.
Family and domain databases
InterPro	IPR020959. ArabinofuranosylTrfase_AftA_C. IPR020963. ArabinofuranosylTrfase_AftA_N. [Graphical view]
KO	K13686.
Pfam	PF12249. AftA_C. 1 hit. PF12250. AftA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AFTA_MYCTU

Accession

Primary (citable) accession number: P72058
Secondary accession number(s): Q8VIT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program