P72030 (EMBB_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable arabinosyltransferase B
EC=2.4.2.-

Gene names

Name:	embB
Ordered Locus Names:	Rv3795, MT3902
ORF Names:	MTCY13D12.29

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	1098 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Miscellaneous	This is one of the targets of the anti-tuberculosis drug ethambutol [(S,S')-2,2'-(ethylenediimino)di-1-butanol; EMB]. EMB is a first-line drug used to treat tuberculosis. EMB inhibits the transfer of arabinogalactan into the cell wall.
Sequence similarities	Belongs to the emb family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW growth Inferred from mutant phenotype. Source: MTBBASE peptidoglycan biosynthetic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from direct assay. Source: MTBBASE integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	transferase activity, transferring pentosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1098	1098	Probable arabinosyltransferase B		PRO_0000220569
Regions
	Transmembrane	28 – 50	23	Helical; Potential
	Transmembrane	217 – 239	23	Helical; Potential
	Transmembrane	271 – 293	23	Helical; Potential
	Transmembrane	402 – 419	18	Helical; Potential
	Transmembrane	434 – 456	23	Helical; Potential
	Transmembrane	472 – 494	23	Helical; Potential
	Transmembrane	541 – 558	18	Helical; Potential
	Transmembrane	570 – 587	18	Helical; Potential
	Transmembrane	597 – 619	23	Helical; Potential
	Transmembrane	626 – 648	23	Helical; Potential
	Transmembrane	663 – 685	23	Helical; Potential
	Transmembrane	698 – 720	23	Helical; Potential
Natural variations
	Natural variant	297	1	S → A Resistance to EMB.
	Natural variant	306	1	M → I Resistance to EMB. Ref.4 Ref.6
	Natural variant	306	1	M → L Resistance to EMB. Ref.4 Ref.6
	Natural variant	306	1	M → V Resistance to EMB. Ref.4 Ref.6
	Natural variant	328	1	D → G Resistance to EMB.
	Natural variant	328	1	D → Y Resistance to EMB.
	Natural variant	330	1	F → V Resistance to EMB. Ref.4
	Natural variant	334	1	Y → H Resistance to EMB.
	Natural variant	406	1	G → A Resistance to EMB.
	Natural variant	406	1	G → C Resistance to EMB.
	Natural variant	406	1	G → D Resistance to EMB.
	Natural variant	497	1	Q → K Resistance to EMB.
	Natural variant	497	1	Q → R Resistance to EMB.
	Natural variant	745	1	G → D Resistance to EMB.
	Natural variant	959	1	D → A Resistance to EMB.
	Natural variant	1000	1	M → R Resistance to EMB.
	Natural variant	1024	1	D → N Resistance to EMB.
Experimental info
	Sequence conflict	773 – 774	2	SW → FL Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P72030 [UniParc]. Last modified July 1, 1997. Version 2. Checksum: DD7D7025DC803833	FASTA	1,098	118,021
	10 20 30 40 50 60 MTQCASRRKS TPNRAILGAF ASARGTRWVA TIAGLIGFVL SVATPLLPVV QTTAMLDWPQ 70 80 90 100 110 120 RGQLGSVTAP LISLTPVDFT ATVPCDVVRA MPPAGGVVLG TAPKQGKDAN LQALFVVVSA 130 140 150 160 170 180 QRVDVTDRNV VILSVPREQV TSPQCQRIEV TSTHAGTFAN FVGLKDPSGA PLRSGFPDPN 190 200 210 220 230 240 LRPQIVGVFT DLTGPAPPGL AVSATIDTRF STRPTTLKLL AIIGAIVATV VALIALWRLD 250 260 270 280 290 300 QLDGRGSIAQ LLLRPFRPAS SPGGMRRLIP ASWRTFTLTD AVVIFGFLLW HVIGANSSDD 310 320 330 340 350 360 GYILGMARVA DHAGYMSNYF RWFGSPEDPF GWYYNLLALM THVSDASLWM RLPDLAAGLV 370 380 390 400 410 420 CWLLLSREVL PRLGPAVEAS KPAYWAAAMV LLTAWMPFNN GLRPEGIIAL GSLVTYVLIE 430 440 450 460 470 480 RSMRYSRLTP AALAVVTAAF TLGVQPTGLI AVAALVAGGR PMLRILVRRH RLVGTLPLVS 490 500 510 520 530 540 PMLAAGTVIL TVVFADQTLS TVLEATRVRA KIGPSQAWYT ENLRYYYLIL PTVDGSLSRR 550 560 570 580 590 600 FGFLITALCL FTAVFIMLRR KRIPSVARGP AWRLMGVIFG TMFFLMFTPT KWVHHFGLFA 610 620 630 640 650 660 AVGAAMAALT TVLVSPSVLR WSRNRMAFLA ALFFLLALCW ATTNGWWYVS SYGVPFNSAM 670 680 690 700 710 720 PKIDGITVST IFFALFAIAA GYAAWLHFAP RGAGEGRLIR ALTTAPVPIV AGFMAAVFVA 730 740 750 760 770 780 SMVAGIVRQY PTYSNGWSNV RAFVGGCGLA DDVLVEPDTN AGFMKPLDGD SGSWGPLGPL 790 800 810 820 830 840 GGVNPVGFTP NGVPEHTVAE AIVMKPNQPG TDYDWDAPTK LTSPGINGST VPLPYGLDPA 850 860 870 880 890 900 RVPLAGTYTT GAQQQSTLVS AWYLLPKPDD GHPLVVVTAA GKIAGNSVLH GYTPGQTVVL 910 920 930 940 950 960 EYAMPGPGAL VPAGRMVPDD LYGEQPKAWR NLRFARAKMP ADAVAVRVVA EDLSLTPEDW 970 980 990 1000 1010 1020 IAVTPPRVPD LRSLQEYVGS TQPVLLDWAV GLAFPCQQPM LHANGIAEIP KFRITPDYSA 1030 1040 1050 1060 1070 1080 KKLDTDTWED GTNGGLLGIT DLLLRAHVMA TYLSRDWARD WGSLRKFDTL VDAPPAQLEL 1090 GTATRSGLWS PGKIRIGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol." Telenti A., Philipp W.J., Sreevatsan S., Bernasconi C., Stockbauer K.E., Wieles B., Musser J.M., Jacobs W.R. Jr. Nat. Med. 3:567-570(1997) [PubMed: 9142129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[4]	"Ethambutol resistance in Mycobacterium tuberculosis: critical role of embB mutations." Sreevatsan S., Stockbauer K.E., Pan X., Kreiswirth B.N., Moghazeh S.L., Jacobs W.R. Jr., Telenti A., Musser J.M. Antimicrob. Agents Chemother. 41:1677-1681(1997) [PubMed: 9257740] [Abstract] Cited for: VARIANTS EMB RESISTANT LEU-306; ILE-306; VAL-306 AND VAL-330.
[5]	"Molecular genetic analysis of nucleotide polymorphisms associated with ethambutol resistance in human isolates of Mycobacterium tuberculosis." Ramaswamy S.V., Amin A.G., Goeksel S., Stager C.E., Dou S.-J., El Sahly H., Moghazeh S.L., Kreiswirth B.N., Musser J.M. Antimicrob. Agents Chemother. 44:326-336(2000) [PubMed: 10639358] [Abstract] Cited for: VARIANTS EMB RESISTANT.
[6]	"Detection of embB codon 306 mutations in ethambutol resistant Mycobacterium tuberculosis directly from sputum samples: a low-cost, rapid approach." Rinder H., Mieskes K.T., Tortoli E., Richter E., Casal M., Vaquero M., Cambau E., Feldmann K., Loescher T. Mol. Cell. Probes 15:37-42(2001) [PubMed: 11162078] [Abstract] Cited for: VARIANTS EMB RESISTANT LEU-306; ILE-306 AND VAL-306.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842584 Genomic DNA. Translation: CAB02474.1. U68480 Genomic DNA. Translation: AAC45281.1. AE000516 Genomic DNA. Translation: AAK48268.1.
PIR	G70697.
RefSeq	NP_218312.1. NC_000962.2. NP_338454.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P72030.
ModBase	Search...
Protein family/group databases
CAZy	GT53. Glycosyltransferase Family 53.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000000734; EBMYCP00000000734; EBMYCG00000000734. EBMYCT00000071975; EBMYCP00000070034; EBMYCG00000071970.
GeneID	886126. 926343.
GenomeReviews	Gene locus MT3902 in contig AE000516_GR. Gene locus Rv3795 in contig AL123456_GR.
KEGG	mtc:MT3902. mtu:Rv3795.
PATRIC	18130341. VBIMycTub22151_4258.
TIGR	MT3902.
Organism-specific databases
TubercuList	Rv3795.
Phylogenomic databases
GeneTree	EBGT00070000032055.
HOGENOM	HBG429120.
OMA	TEIPKFR.
ProtClustDB	CLSK2748679.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-15270.
Family and domain databases
InterPro	IPR007680. Mycobac_arabinan_synth. [Graphical view]
KO	K11386.
Pfam	PF04602. Arabinose_trans. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00330. Ethambutol.

Entry information

Entry name

EMBB_MYCTU

Accession

Primary (citable) accession number: P72030
Secondary accession number(s): P72061

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	July 1, 1997
Last modified:	January 25, 2012
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program