Reviewed,
UniProtKB/Swiss-Prot P72030 (EMBB_MYCTU)
Last modified
June 16, 2009.
Version 69.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Probable arabinosyltransferase B EC=2.4.2.- | ||||||
| Gene names |
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| Organism | Mycobacterium tuberculosis [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 1773 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex |
Protein attributes
| Sequence length | 1098 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan. |
| Subcellular location | Cell membrane; Multi-pass membrane protein Probable. |
| Miscellaneous | This is one of the targets of the anti-tuberculosis drug ethambutol [(S,S')-2,2'-(ethylenediimino)di-1-butanol; EMB]. EMB is a first-line drug used to treat tuberculosis. EMB inhibits the transfer of arabinogalactan into the cell wall. |
| Sequence similarities | Belongs to the emb family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Antibiotic resistance Cell wall biogenesis/degradation |
| Cellular component | Cell membrane Membrane |
| Domain | Transmembrane |
| Molecular function | Glycosyltransferase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic processInferred from electronic annotation. Source: InterPro response to antibioticInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | transferase activity, transferring pentosyl groups Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1098 | 1098 | Probable arabinosyltransferase B | PRO_0000220569 | |||||
Regions | |||||||||
| Transmembrane | 28 – 50 | 23 | Potential | ||||||
| Transmembrane | 217 – 239 | 23 | Potential | ||||||
| Transmembrane | 271 – 293 | 23 | Potential | ||||||
| Transmembrane | 402 – 419 | 18 | Potential | ||||||
| Transmembrane | 434 – 456 | 23 | Potential | ||||||
| Transmembrane | 472 – 494 | 23 | Potential | ||||||
| Transmembrane | 541 – 558 | 18 | Potential | ||||||
| Transmembrane | 570 – 587 | 18 | Potential | ||||||
| Transmembrane | 597 – 619 | 23 | Potential | ||||||
| Transmembrane | 626 – 648 | 23 | Potential | ||||||
| Transmembrane | 663 – 685 | 23 | Potential | ||||||
| Transmembrane | 698 – 720 | 23 | Potential | ||||||
Natural variations | |||||||||
| Natural variant | 297 | 1 | S → A Resistance to EMB. | ||||||
| Natural variant | 306 | 1 | M → I Resistance to EMB. Ref.4 Ref.6 | ||||||
| Natural variant | 306 | 1 | M → L Resistance to EMB. Ref.4 Ref.6 | ||||||
| Natural variant | 306 | 1 | M → V Resistance to EMB. Ref.4 Ref.6 | ||||||
| Natural variant | 328 | 1 | D → G Resistance to EMB. | ||||||
| Natural variant | 328 | 1 | D → Y Resistance to EMB. | ||||||
| Natural variant | 330 | 1 | F → V Resistance to EMB. Ref.4 | ||||||
| Natural variant | 334 | 1 | Y → H Resistance to EMB. | ||||||
| Natural variant | 406 | 1 | G → A Resistance to EMB. | ||||||
| Natural variant | 406 | 1 | G → C Resistance to EMB. | ||||||
| Natural variant | 406 | 1 | G → D Resistance to EMB. | ||||||
| Natural variant | 497 | 1 | Q → K Resistance to EMB. | ||||||
| Natural variant | 497 | 1 | Q → R Resistance to EMB. | ||||||
| Natural variant | 745 | 1 | G → D Resistance to EMB. | ||||||
| Natural variant | 959 | 1 | D → A Resistance to EMB. | ||||||
| Natural variant | 1000 | 1 | M → R Resistance to EMB. | ||||||
| Natural variant | 1024 | 1 | D → N Resistance to EMB. | ||||||
Experimental info | |||||||||
| Sequence conflict | 773 – 774 | 2 | SW → FL Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol." Telenti A., Philipp W.J., Sreevatsan S., Bernasconi C., Stockbauer K.E., Wieles B., Musser J.M., Jacobs W.R. Jr. Nat. Med. 3:567-570(1997) [PubMed: 9142129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv. |
| [2] | "Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. Barrell B.G.Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv. |
| [3] | "Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. Fraser C.M.J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh. |
| [4] | "Ethambutol resistance in Mycobacterium tuberculosis: critical role of embB mutations." Sreevatsan S., Stockbauer K.E., Pan X., Kreiswirth B.N., Moghazeh S.L., Jacobs W.R. Jr., Telenti A., Musser J.M. Antimicrob. Agents Chemother. 41:1677-1681(1997) [PubMed: 9257740] [Abstract] Cited for: VARIANTS EMB RESISTANT LEU-306; ILE-306; VAL-306 AND VAL-330. |
| [5] | "Molecular genetic analysis of nucleotide polymorphisms associated with ethambutol resistance in human isolates of Mycobacterium tuberculosis." Ramaswamy S.V., Amin A.G., Goeksel S., Stager C.E., Dou S.-J., El Sahly H., Moghazeh S.L., Kreiswirth B.N., Musser J.M. Antimicrob. Agents Chemother. 44:326-336(2000) [PubMed: 10639358] [Abstract] Cited for: VARIANTS EMB RESISTANT. |
| [6] | "Detection of embB codon 306 mutations in ethambutol resistant Mycobacterium tuberculosis directly from sputum samples: a low-cost, rapid approach." Rinder H., Mieskes K.T., Tortoli E., Richter E., Casal M., Vaquero M., Cambau E., Feldmann K., Loescher T. Mol. Cell. Probes 15:37-42(2001) [PubMed: 11162078] [Abstract] Cited for: VARIANTS EMB RESISTANT LEU-306; ILE-306 AND VAL-306. |
Cross-references
Sequence databases | |
|---|---|
| BX842584 Genomic DNA. Translation: CAB02474.1. U68480 Genomic DNA. Translation: AAC45281.1. AE000516 Genomic DNA. Translation: AAK48268.1. | |
| PIR | G70697. |
| RefSeq | NP_218312.1. NP_338454.1. |
3D structure databases | |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GT53. Glycosyltransferase Family 53. |
Genome annotation databases | |
| GeneID | 886126. 926343. |
| GenomeReviews | Gene locus MT3902 in contig AE000516_GR. Gene locus Rv3795 in contig AL123456_GR. |
| KEGG | mtc:MT3902. mtu:Rv3795. |
| TIGR | MT3902. |
Organism-specific databases | |
| TubercuList | Rv3795. |
Phylogenomic databases | |
| HOGENOM | P72030. |
| OMA | P72030. NGWANIR. |
Family and domain databases | |
| InterPro | IPR007680. Arab_transf. [Graphical view] |
| Pfam | PF04602. Arabinose_trans. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| DrugBank | DB00330. Ethambutol. |
Entry information
| Entry name | EMBB_MYCTU | ||||||||
| Accession | Primary (citable) accession number: P72030 Secondary accession number(s): P72061 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

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