Reviewed, UniProtKB/Swiss-Prot P71989 (GABD1_MYCTU)

Names and origin

Protein names

Recommended name:
    Succinate-semialdehyde dehydrogenase [NADP+] 1
      Short name=SSADH 1
      Short name=SSDH 1
    EC=1.2.1.16

Gene names

Name:	gabD1
Ordered Locus Names:	Rv0234c, MT0245

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the NADP+-dependent oxidation of succinate semialdehyde to succinate. It is believed to be the main source of succinate semialdehyde dehydrogenase activity in Mycobacterium. NAD+ can substitute for NADP+, but enzymatic activity is three times reduced. Ref.3
Catalytic activity	Succinate semialdehyde + NAD(P)+ + H2O = succinate + NAD(P)H. Ref.3
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinate-semialdehyde dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Succinate-semialdehyde dehydrogenase [NADP+] 1		PRO_0000310705
Regions
	Nucleotide binding	209 – 214	6	NAD or NADP By similarity
	Compositional bias	19 – 117	99	Ala-rich
Sites
	Active site	231	1	By similarity
	Active site	265	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P71989-1 [UniParc]. Last modified November 13, 2007. Version 3. Checksum: B391266C5441A05F	FASTA	457	48,545
	10 20 30 40 50 60 MPIATINPAT GETVKTFTAA TDDEVDAAIA RAHRRFADYR QTSFAQRARW ANATADLLEA 70 80 90 100 110 120 EADQAAAMMT LEMGKTLAAA KAEALKCAKG FRYYAENAEA LLADEPADAA KVGASAAYGR 130 140 150 160 170 180 YQPLGVILAV MPWNFPLWQA VRFAAPALMA GNVGLLKHAS NVPQCALYLA DVIARGGFPD 190 200 210 220 230 240 GCFQTLLVSS GAVEAILRDP RVAAATLTGS EPAGQSVGAI AGNEIKPTVL ELGGSDPFIV 250 260 270 280 290 300 MPSADLDAAV STAVTGRVQN NGQSCIAAKR FIVHADIYDD FVDKFVARMA ALRVGDPTDP 310 320 330 340 350 360 DTDVGPLATE QGRNEVAKQV EDAAAAGAVI RCGGKRLDRP GWFYPPTVIT DISKDMALYT 370 380 390 400 410 420 EEVFGPVASV FRAANIDEAV EIANATTFGL GSNAWTRDET EQRRFIDDIV AGQVFINGMT 430 440 450 VSYPELPFGG VKRSGYGREL SAHGIREFCN IKTVWIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Variant tricarboxylic acid cycle in Mycobacterium tuberculosis: identification of alpha-ketoglutarate decarboxylase." Tian J., Bryk R., Itoh M., Suematsu M., Nathan C. Proc. Natl. Acad. Sci. U.S.A. 102:10670-10675(2005) [PubMed: 16027371] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, ROLE IN TRICARBOXYLIC ACID CYCLE. Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842572 Genomic DNA. Translation: CAB03694.2. Different initiation. AE000516 Genomic DNA. Translation: AAK44465.1. Different initiation.
PIR	F70687.
RefSeq	NP_216247.2. NP_334651.1.
3D structure databases
HSSP	HSSP built from PDB template 1AG8 based on UniProtKB P20000.
ModBase	Search...
Genome annotation databases
GeneID	886732. 923143.
GenomeReviews	Gene locus MT0245 in contig AE000516_GR. Gene locus Rv0234c in contig AL123456_GR.
KEGG	mtc:MT0245. mtu:Rv0234c.
TIGR	MT0245.
Organism-specific databases
TubercuList	Rv0234c.
Phylogenomic databases
HOGENOM	P71989.
OMA	P71989. VPQSALY.
Enzyme and pathway databases
BRENDA	1.2.1.16. 809.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GABD1_MYCTU

Accession

Primary (citable) accession number: P71989
Secondary accession number(s): Q7DA77

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 13, 2007
Last modified:	June 16, 2009
This is version 54 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)