Reviewed, UniProtKB/Swiss-Prot P71880 (MAOX_MYCTU)

Names and origin

Protein names

Recommended name:
    Putative malate oxidoreductase [NAD]
    EC=1.1.1.38
Alternative name(s):
    Malic enzyme

Gene names

Name:	mez
Ordered Locus Names:	Rv2332, MT2394
ORF Names:	MTCY3G12.02c, MTCY98.01

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	Putative malate oxidoreductase [NAD]		PRO_0000160248
Sites
	Active site	96	1	Proton donor By similarity
	Active site	169	1	Proton acceptor By similarity
	Metal binding	240	1	Divalent metal cation By similarity
	Metal binding	241	1	Divalent metal cation By similarity
	Metal binding	264	1	Divalent metal cation By similarity
	Binding site	264	1	NAD By similarity
	Binding site	410	1	NAD By similarity
	Site	264	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P71880-1 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: 407248FE6BDFC875	FASTA	548	59,423
	10 20 30 40 50 60 MSDARVPRIP AALSAPSLNR GVGFTHAQRR RLGLTGRLPS AVLTLDQQAE RVWHQLQSLA 70 80 90 100 110 120 TELGRNLLLE QLHYRHEVLY FKVLADHLPE LMPVVYTPTV GEAIQRFSDE YRGQRGLFLS 130 140 150 160 170 180 IDEPDEIEEA FNTLGLGPED VDLIVCTDAE AILGIGDWGV GGIQIAVGKL ALYTAGGGVD 190 200 210 220 230 240 PRRCLAVSLD VGTDNEQLLA DPFYLGNRHA RRRGREYDEF VSRYIETAQR LFPRAILHFE 250 260 270 280 290 300 DFGPANARKI LDTYGTDYCV FNDDMQGTGA VVLAAVYSGL KVTGIPLRDQ TIVVFGAGTA 310 320 330 340 350 360 GMGIADQIRD AMVADGATLE QAVSQIWPID RPGLLFDDMD DLRDFQVPYA KNRHQLGVAV 370 380 390 400 410 420 GDRVGLSDAI KIASPTILLG CSTVYGAFTK EVVEAMTASC KHPMIFPLSN PTSRMEAIPA 430 440 450 460 470 480 DVLAWSNGRA LLATGSPVAP VEFDETTYVI GQANNVLAFP GIGLGVIVAG ARLITRRMLH 490 500 510 520 530 540 AAAKAIAHQA NPTNPGDSLL PDVQNLRAIS TTVAEAVYRA AVQDGVASRT HDDVRQAIVD TMWLPAYD

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842579 Genomic DNA. Translation: CAB02059.2. AE000516 Genomic DNA. Translation: AAK46686.1.
PIR	E70705.
RefSeq	NP_216848.2. NP_336872.1.
3D structure databases
HSSP	HSSP built from PDB template 1LLQ based on UniProtKB P27443.
ModBase	Search...
Genome annotation databases
GeneID	887962. 924018.
GenomeReviews	Gene locus MT2394 in contig AE000516_GR. Gene locus Rv2332 in contig AL123456_GR.
KEGG	mtc:MT2394. mtu:Rv2332.
TIGR	MT2394.
Organism-specific databases
TubercuList	Rv2332.
Phylogenomic databases
HOGENOM	P71880.
OMA	P71880. MISAAVD.
Enzyme and pathway databases
BRENDA	1.1.1.38. 809.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAOX_MYCTU

Accession

Primary (citable) accession number: P71880

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 29, 2001
Last modified:	June 16, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)