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Protein

3-oxosteroid 1-dehydrogenase

Gene

kstD

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3-ketosteroids. Is also involved in the formation of 3-keto-1,4-diene-steroid from 3-keto-4-ene-steroid.1 Publication

Catalytic activityi

A 3-oxosteroid + acceptor = a 3-oxo-Delta(1)-steroid + reduced acceptor.1 Publication
A 3-oxo-Delta(4)-steroid + acceptor = a 3-oxo-Delta(1,4)-steroid + reduced acceptor.1 Publication

Cofactori

FADBy similarity

Kineticsi

  1. KM=33 µM for 5alpha-testosterone (5alpha-T)(at pH 7.4 and 30 degrees Celsius)1 Publication
  2. KM=65 µM for progesterone (at pH 7.4 and 30 degrees Celsius)1 Publication
  3. KM=166 µM for 5alpha-androstane-3,17-dione (5alpha-AD)(at pH 7.4 and 30 degrees Celsius)1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi7 – 36FADBy similarityAdd BLAST30

    GO - Molecular functioni

    GO - Biological processi

    • anaerobic respiration Source: GO_Central
    • cholesterol catabolic process Source: MTBBASE
    • steroid biosynthetic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism, Steroid metabolism

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciMetaCyc:G185E-7814-MONOMER.
    MTBH37RV:G185E-7814-MONOMER.

    Chemistry databases

    SwissLipidsiSLP:000001004.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-oxosteroid 1-dehydrogenase (EC:1.3.99.4)
    Alternative name(s):
    3-keto-Delta(4)-steroid Delta(1)-dehydrogenase
    Short name:
    KSDD
    3-oxo-Delta(4)-steroid 1-dehydrogenase
    Short name:
    KSTD
    Gene namesi
    Name:kstD
    Ordered Locus Names:Rv3537
    OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    Taxonomic identifieri83332 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    Proteomesi
    • UP000001584 Componenti: Chromosome

    Organism-specific databases

    TubercuListiRv3537.

    Subcellular locationi

    GO - Cellular componenti

    • plasma membrane Source: MTBBASE
    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004039521 – 5633-oxosteroid 1-dehydrogenaseAdd BLAST563

    Proteomic databases

    PaxDbiP71864.

    Interactioni

    Protein-protein interaction databases

    STRINGi83332.Rv3537.

    Structurei

    3D structure databases

    ProteinModelPortaliP71864.
    SMRiP71864.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105DAB. Bacteria.
    COG1053. LUCA.
    HOGENOMiHOG000245848.
    InParanoidiP71864.
    KOiK05898.
    OMAiIEKAPHF.
    PhylomeDBiP71864.

    Family and domain databases

    Gene3Di3.50.50.60. 3 hits.
    3.90.700.10. 1 hit.
    InterProiIPR003953. FAD-binding_2.
    IPR023753. FAD/NAD-binding_dom.
    IPR027477. Succ_DH/fumarate_Rdtase_cat.
    [Graphical view]
    PfamiPF00890. FAD_binding_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF51905. SSF51905. 3 hits.
    SSF56425. SSF56425. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P71864-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTVQEFDVVV VGSGAAGMVA ALVAAHRGLS TVVVEKAPHY GGSTARSGGG
    60 70 80 90 100
    VWIPNNEVLK RRGVRDTPEA ARTYLHGIVG EIVEPERIDA YLDRGPEMLS
    110 120 130 140 150
    FVLKHTPLKM CWVPGYSDYY PEAPGGRPGG RSIEPKPFNA RKLGADMAGL
    160 170 180 190 200
    EPAYGKVPLN VVVMQQDYVR LNQLKRHPRG VLRSMKVGAR TMWAKATGKN
    210 220 230 240 250
    LVGMGRALIG PLRIGLQRAG VPVELNTAFT DLFVENGVVS GVYVRDSHEA
    260 270 280 290 300
    ESAEPQLIRA RRGVILACGG FEHNEQMRIK YQRAPITTEW TVGASANTGD
    310 320 330 340 350
    GILAAEKLGA ALDLMDDAWW GPTVPLVGKP WFALSERNSP GSIIVNMSGK
    360 370 380 390 400
    RFMNESMPYV EACHHMYGGE HGQGPGPGEN IPAWLVFDQR YRDRYIFAGL
    410 420 430 440 450
    QPGQRIPSRW LDSGVIVQAD TLAELAGKAG LPADELTATV QRFNAFARSG
    460 470 480 490 500
    VDEDYHRGES AYDRYYGDPS NKPNPNLGEV GHPPYYGAKM VPGDLGTKGG
    510 520 530 540 550
    IRTDVNGRAL RDDGSIIDGL YAAGNVSAPV MGHTYPGPGG TIGPAMTFGY
    560
    LAALHIADQA GKR
    Length:563
    Mass (Da):60,636
    Last modified:July 5, 2004 - v3
    Checksum:iE5548D090D7A25A8
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL123456 Genomic DNA. Translation: CCP46359.1.
    PIRiB70676.
    RefSeqiNP_218054.1. NC_000962.3.
    WP_003419253.1. NZ_KK339374.1.

    Genome annotation databases

    EnsemblBacteriaiCCP46359; CCP46359; Rv3537.
    GeneIDi888422.
    KEGGimtu:Rv3537.
    PATRICi18156482. VBIMycTub87468_3951.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL123456 Genomic DNA. Translation: CCP46359.1.
    PIRiB70676.
    RefSeqiNP_218054.1. NC_000962.3.
    WP_003419253.1. NZ_KK339374.1.

    3D structure databases

    ProteinModelPortaliP71864.
    SMRiP71864.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi83332.Rv3537.

    Chemistry databases

    SwissLipidsiSLP:000001004.

    Proteomic databases

    PaxDbiP71864.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCCP46359; CCP46359; Rv3537.
    GeneIDi888422.
    KEGGimtu:Rv3537.
    PATRICi18156482. VBIMycTub87468_3951.

    Organism-specific databases

    TubercuListiRv3537.

    Phylogenomic databases

    eggNOGiENOG4105DAB. Bacteria.
    COG1053. LUCA.
    HOGENOMiHOG000245848.
    InParanoidiP71864.
    KOiK05898.
    OMAiIEKAPHF.
    PhylomeDBiP71864.

    Enzyme and pathway databases

    BioCyciMetaCyc:G185E-7814-MONOMER.
    MTBH37RV:G185E-7814-MONOMER.

    Family and domain databases

    Gene3Di3.50.50.60. 3 hits.
    3.90.700.10. 1 hit.
    InterProiIPR003953. FAD-binding_2.
    IPR023753. FAD/NAD-binding_dom.
    IPR027477. Succ_DH/fumarate_Rdtase_cat.
    [Graphical view]
    PfamiPF00890. FAD_binding_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF51905. SSF51905. 3 hits.
    SSF56425. SSF56425. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry namei3O1D1_MYCTU
    AccessioniPrimary (citable) accession number: P71864
    Secondary accession number(s): L0TD13
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2011
    Last sequence update: July 5, 2004
    Last modified: November 30, 2016
    This is version 107 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
      Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.