Carbamoyl-phosphate synthase small chain - Mycobacterium tuberculosis

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Reviewed, UniProtKB/Swiss-Prot P71811 (CARA_MYCTU)

Last modified June 16, 2009. Version 77. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	Rv1383, MT1427
ORF Names:	MTCY02B12.17

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

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Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO₃^- + H₂O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

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Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 376

376

Carbamoyl-phosphate synthase small chain HAMAP MF_01209

PRO_0000112296

Regions

Domain

184 – 376

193

Glutamine amidotransferase type-1

Region

1 – 181

181

CPSase HAMAP MF_01209

Sites

Active site

260

Nucleophile By similarity

Active site

350

By similarity

Active site

352

By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P71811-1 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: DF057465F47AE46D
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FASTA

376

39,767

        10         20         30         40         50         60 
MSKAVLVLED GRVFTGRPFG ATGQALGEAV FSTGMSGYQE TLTDPSYHRQ IVVATAPQIG 

        70         80         90        100        110        120 
NTGWNGEDSE SRGERIWVAG YAVRDPSPRA SNWRATGTLE DELIRQRIVG IAGIDTRAVV 

       130        140        150        160        170        180 
RHLRSRGSMK AGVFSDGALA EPADLIARVR AQQSMLGADL AGEVSTAEPY VVEPDGPPGV 

       190        200        210        220        230        240 
SRFTVAALDL GIKTNTPRNF ARRGIRCHVL PASTTFEQIA ELNPHGVFLS NGPGDPATAD 

       250        260        270        280        290        300 
HVVALTREVL GAGIPLFGIC FGNQILGRAL GLSTYKMVFG HRGINIPVVD HATGRVAVTA 

       310        320        330        340        350        360 
QNHGFALQGE AGQSFATPFG PAVVSHTCAN DGVVEGVKLV DGRAFSVQYH PEAAAGPHDA 

       370 
EYLFDQFVEL MAGEGR

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References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence."
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.

Barrell B.G.
Nature 393:537-544(1998) [PubMed: 9634230] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains."
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R.

Fraser C.M.
J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CDC 1551 / Oshkosh.

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Cross-references

Sequence databases
	BX842576 Genomic DNA. Translation: CAB02644.1. AE000516 Genomic DNA. Translation: AAK45692.1. Different initiation.
PIR	D70959.
RefSeq	NP_215899.1. NP_335878.1.
3D structure databases
HSSP	HSSP built from PDB template 1CE8 based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	886761. 924557.
GenomeReviews	Gene locus MT1427 in contig AE000516_GR. Gene locus Rv1383 in contig AL123456_GR.
KEGG	mtc:MT1427. mtu:Rv1383.
TIGR	MT1427.
Organism-specific databases
TubercuList	Rv1383.
Phylogenomic databases
HOGENOM	P71811.
OMA	P71811. LFDGSNC.
Enzyme and pathway databases
BRENDA	6.3.5.5. 809.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

CARA_MYCTU

Accession

Primary (citable) accession number: P71811

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents