Probable oxygen-independent coproporphyrinogen III oxidase

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Reviewed, UniProtKB/Swiss-Prot P71756 (HEMN_MYCTU)

Last modified November 25, 2008. Version 65. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Probable oxygen-independent coproporphyrinogen III oxidase
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.99.22

Gene names

Name:	hemN
Ordered Locus Names:	Rv2388c, MT2457
ORF Names:	MTCY253.33

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

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Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX By similarity.
Catalytic activity	Coproporphyrinogen-III + 2 S-adenosyl-L-methionine = protoporphyrinogen-IX + 2 CO(2) + 2 L-methionine + 2 5'-deoxyadenosine.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen III (AdoMet route): step 1/1.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the anaerobic coproporphyrinogen III oxidase family.

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Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW coproporphyrinogen dehydrogenase activity Inferred from electronic annotation. Source: EC coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 375

375

Probable oxygen-independent coproporphyrinogen III oxidase

PRO_0000109944

Regions

Region

68 – 69

S-adenosyl-L-methionine 2 binding By similarity

Sites

Metal binding

Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Metal binding

Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Metal binding

Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Binding site

S-adenosyl-L-methionine 1 By similarity

Binding site

S-adenosyl-L-methionine 2; via carbonyl oxygen By similarity

Binding site

S-adenosyl-L-methionine 1; via amide nitrogen and carbonyl oxygen By similarity

Binding site

100

S-adenosyl-L-methionine 1 By similarity

Binding site

127

S-adenosyl-L-methionine 2 By similarity

Binding site

139

S-adenosyl-L-methionine 2 By similarity

Binding site

164

S-adenosyl-L-methionine 2 By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P71756-1 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 6E614EF58AA8FCE9
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FASTA

375

40,334

        10         20         30         40         50         60 
MPGQPFGVYL HVPFCLTRCG YCDFNTYTPA QLGGVSPDRW LLALRAELEL AAAKLDAPTV 

        70         80         90        100        110        120 
HTVYVGGGTP SLLGGERLAT LLDMVRDHFV LAPDAEVSTE ANPESTWPEF FATIRAAGYT 

       130        140        150        160        170        180 
RVSLGMQSVA PRVLATLDRV HSPGRAAAAA TEAIAEGFTH VNLDLIYGTP GESDDDLVRS 

       190        200        210        220        230        240 
VDAAVQAGVD HVSAYALVVE HGTALARRVR RGELAAPDDD VLAHRYELVD ARLSAAGFAW 

       250        260        270        280        290        300 
YEVSNWCRPG GECRHNLGYW DGGQWWGAGP GAHGYIGVTR WWNVKHPNTY AEILAGATLP 

       310        320        330        340        350        360 
VAGFEQLGAD ALHTEDVLLK VRLRQGLPLA RLGAAERERA EAVLADGLLD YHGDRLVLTG 

       370 
RGRLLADAVV RTLLG

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References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence."
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.

Barrell B.G.
Nature 393:537-544(1998) [PubMed: 9634230] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains."
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R.

Fraser C.M.
J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CDC 1551 / Oshkosh.

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Cross-references

Sequence databases
	BX842579 Genomic DNA. Translation: CAB03737.1. AE000516 Genomic DNA. Translation: AAK46752.1. Different initiation.
PIR	G70681.
RefSeq	NP_216904.1. NP_336938.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	885300. 925891.
GenomeReviews	Gene locus MT2457 in contig AE000516_GR. Gene locus Rv2388c in contig AL123456_GR.
KEGG	mtc:MT2457. mtu:Rv2388c.
TIGR	MT2457.
Organism-specific databases
TubercuList	Rv2388c.
Phylogenomic databases
HOGENOM	P71756.
Family and domain databases
InterPro	IPR006638. Elp3/MiaB/NifB. IPR010723. HemN_C. IPR004559. HemN_rel. IPR007197. Radical_SAM. [Graphical view]
Pfam	PF06969. HemN_C. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00539. hemN_rel. 1 hit.
ProtoNet	Search...

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Entry information

Entry name

HEMN_MYCTU

Accession

Primary (citable) accession number: P71756

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 25, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents