Replicative DNA helicase - Mycobacterium tuberculosis

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P71715 (DNAB_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Replicative DNA helicase
EC=3.6.4.12

Cleaved into the following chain:

Endonuclease PI-MtuHIP
EC=3.1.-.-
Alternative name(s):
Mtu dnaB intein

Gene names

Name:	dnaB
Ordered Locus Names:	Rv0058, MT0064
ORF Names:	MTCY21D4.21

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity By similarity. The intein is an endonuclease Potential.
Catalytic activity	ATP + H₂O = ADP + phosphate.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.
Miscellaneous	Was identified as a high-confidence drug target.
Sequence similarities	Belongs to the helicase family. DnaB subfamily. Contains 1 DOD-type homing endonuclease domain. Contains 1 SF4 helicase domain.

Ontologies

Keywords
Biological process	DNA replication Intron homing
Cellular component	Primosome
Domain	Repeat
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Endonuclease Helicase Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA duplex unwinding Inferred from electronic annotation. Source: GOC DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW growth Inferred from mutant phenotype PubMed 12657046. Source: MTBBASE intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC response to DNA damage stimulus Inferred from expression pattern PubMed 11443079. Source: MTBBASE
Cellular_component	cell wall Inferred from direct assay PubMed 15525680. Source: MTBBASE cytosol Inferred from direct assay PubMed 15525680. Source: MTBBASE replisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 399

399

Replicative DNA helicase, 1st part

PRO_0000013282

Chain

400 – 815

416

Endonuclease PI-MtuHIP

PRO_0000013283

Chain

816 – 874

Replicative DNA helicase, 2nd part

PRO_0000013284

Regions

Domain

195 – 462

268

SF4 helicase; first part

Domain

582 – 660

DOD-type homing endonuclease

Domain

612 – 874

263

SF4 helicase; second part

Nucleotide binding

226 – 233

ATP Potential

Experimental info

Sequence conflict

552

R → G in AAK44286. Ref.2

Secondary structure

874

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P71715 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: 894155A86DCB9D70
Show »

FASTA

874

96,917

        10         20         30         40         50         60 
MAVVDDLAPG MDSSPPSEDY GRQPPQDLAA EQSVLGGMLL SKDAIADVLE RLRPGDFYRP 

        70         80         90        100        110        120 
AHQNVYDAIL DLYGRGEPAD AVTVAAELDR RGLLRRIGGA PYLHTLISTV PTAANAGYYA 

       130        140        150        160        170        180 
SIVAEKALLR RLVEAGTRVV QYGYAGAEGA DVAEVVDRAQ AEIYDVADRR LSEDFVALED 

       190        200        210        220        230        240 
LLQPTMDEID AIASSGGLAR GVATGFTELD EVTNGLHPGQ MVIVAARPGV GKSTLGLDFM 

       250        260        270        280        290        300 
RSCSIRHRMA SVIFSLEMSK SEIVMRLLSA EAKIKLSDMR SGRMSDDDWT RLARRMSEIS 

       310        320        330        340        350        360 
EAPLFIDDSP NLTMMEIRAK ARRLRQKANL KLIVVDYLQL MTSGKKYESR QVEVSEFSRH 

       370        380        390        400        410        420 
LKLLAKELEV PVVAISQLNR GPEQRTDKKP MLADLRESGC LTASTRILRA DTGAEVAFGE 

       430        440        450        460        470        480 
LMRSGERPMV WSLDERLRMV ARPMINVFPS GRKEVFRLRL ASGREVEATG SHPFMKFEGW 

       490        500        510        520        530        540 
TPLAQLKVGD RIAAPRRVPE PIDTQRMPES ELISLARMIG DGSCLKNQPI RYEPVDEANL 

       550        560        570        580        590        600 
AAVTVSAAHS DRAAIRDDYL AARVPSLRPA RQRLPRGRCT PIAAWLAGLG LFTKRSHEKC 

       610        620        630        640        650        660 
VPEAVFRAPN DQVALFLRHL WSAGGSVRWD PTNGQGRVYY GSTSRRLIDD VAQLLLRVGI 

       670        680        690        700        710        720 
FSWITHAPKL GGHDSWRLHI HGAKDQVRFL RHVGVHGAEA VAAQEMLRQL KGPVRNPNLD 

       730        740        750        760        770        780 
SAPKKVWAQV RNRLSAKQMM DIQLHEPTMW KHSPSRSRPH RAEARIEDRA IHELARGDAY 

       790        800        810        820        830        840 
WDTVVEITSI GDQHVFDGTV SGTHNFVANG ISLHNSLEQD ADVVILLHRP DAFDRDDPRG 

       850        860        870 
GEADFILAKH RNGPTKTVTV AHQLHLSRFA NMAR

« Hide

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BX842572 Genomic DNA. Translation: CAB02519.1.
AE000516 Genomic DNA. Translation: AAK44286.1.
AL123456 Genomic DNA. Translation: CCP42780.1.

PIR

B70914.

RefSeq

NP_214572.1. NC_000962.3.
NP_334472.1. NC_002755.2.
YP_006513372.1. NC_018143.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2R5U	X-ray	1.90	A/B/C/D/E/F	1-197	[»]

ProteinModelPortal

P71715.

SMR

P71715. Positions 22-497, 771-874.

ModBase

Search...

Protein-protein interaction databases

STRING

83332.Rv0058.

Protein family/group databases

MEROPS

N10.002.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAK44286; AAK44286; MT0064.

GeneID

13316036.
887009.
922737.

KEGG

mtc:MT0064.
mtu:Rv0058.
mtv:RVBD_0058.

PATRIC

18121861. VBIMycTub22151_0065.

Organism-specific databases

TubercuList

Rv0058.

Phylogenomic databases

eggNOG

COG0305.

HOGENOM

HOG000113195.

K02314.

OMA

LPRHAIQ.

ProtClustDB

PRK07773.

Family and domain databases

Gene3D

1.10.860.10. 1 hit.

InterPro

IPR003593. AAA+_ATPase.
IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
[Graphical view]

Pfam

PF00772. DnaB. 1 hit.
PF03796. DnaB_C. 2 hits.
[Graphical view]

PRINTS

PR00379. INTEIN.

SMART

SM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]

SUPFAM

SSF48024. DNA_helicase_DnaB-like_N. 1 hit.

TIGRFAMs

TIGR00665. DnaB. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.

PROSITE

PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

ChEMBL

CHEMBL1741192.

EvolutionaryTrace

P71715.

Entry information

Entry name

DNAB_MYCTU

Accession

Primary (citable) accession number: P71715
Secondary accession number(s): L0T2G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following chain:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents