Reviewed, UniProtKB/Swiss-Prot P71715 (DNAB_MYCTU)

Names and origin

Protein names

Recommended name:
    Replicative DNA helicase
    EC=3.6.1.-
Cleaved into the following chain:
    1- Recommended name:
            Endonuclease PI-MtuHIP
              EC=3.1.-.-
        Alternative name(s):
            Mtu dnaB intein

Gene names

Name:	dnaB
Ordered Locus Names:	Rv0058, MT0064
ORF Names:	MTCY21D4.21

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity By similarity. The intein is an endonuclease Potential.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.
Sequence similarities	Belongs to the helicase family. DnaB subfamily. Contains 1 DOD-type homing endonuclease domain. Contains 1 SF4 helicase domain.

Ontologies

Keywords
Biological process	DNA replication Intron homing
Cellular component	Primosome
Domain	Repeat
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Endonuclease Helicase Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	alpha DNA polymerase:primase complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 399

399

Replicative DNA helicase, 1st part

PRO_0000013282

Chain

400 – 815

416

Endonuclease PI-MtuHIP

PRO_0000013283

Chain

816 – 874

59

Replicative DNA helicase, 2nd part

PRO_0000013284

Regions

Domain

195 – 462

268

SF4 helicase; first part

Domain

582 – 660

79

DOD-type homing endonuclease

Domain

612 – 874

263

SF4 helicase; second part

Nucleotide binding

226 – 233

8

ATP Potential

Experimental info

Sequence conflict

552

1

R → G in AAK44286. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

874

Helix Strand Turn

Details...

Helix

28 – 40

13

Helix

42 – 49

8

Helix

60 – 74

15

Helix

81 – 90

10

Helix

94 – 97

4

Helix

100 – 107

8

Helix

113 – 115

3

Helix

116 – 145

30

Helix

152 – 164

13

Sequences

	Sequence		Length	Mass (Da)
	P71715-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 894155A86DCB9D70	FASTA	874	96,917
	10 20 30 40 50 60 MAVVDDLAPG MDSSPPSEDY GRQPPQDLAA EQSVLGGMLL SKDAIADVLE RLRPGDFYRP 70 80 90 100 110 120 AHQNVYDAIL DLYGRGEPAD AVTVAAELDR RGLLRRIGGA PYLHTLISTV PTAANAGYYA 130 140 150 160 170 180 SIVAEKALLR RLVEAGTRVV QYGYAGAEGA DVAEVVDRAQ AEIYDVADRR LSEDFVALED 190 200 210 220 230 240 LLQPTMDEID AIASSGGLAR GVATGFTELD EVTNGLHPGQ MVIVAARPGV GKSTLGLDFM 250 260 270 280 290 300 RSCSIRHRMA SVIFSLEMSK SEIVMRLLSA EAKIKLSDMR SGRMSDDDWT RLARRMSEIS 310 320 330 340 350 360 EAPLFIDDSP NLTMMEIRAK ARRLRQKANL KLIVVDYLQL MTSGKKYESR QVEVSEFSRH 370 380 390 400 410 420 LKLLAKELEV PVVAISQLNR GPEQRTDKKP MLADLRESGC LTASTRILRA DTGAEVAFGE 430 440 450 460 470 480 LMRSGERPMV WSLDERLRMV ARPMINVFPS GRKEVFRLRL ASGREVEATG SHPFMKFEGW 490 500 510 520 530 540 TPLAQLKVGD RIAAPRRVPE PIDTQRMPES ELISLARMIG DGSCLKNQPI RYEPVDEANL 550 560 570 580 590 600 AAVTVSAAHS DRAAIRDDYL AARVPSLRPA RQRLPRGRCT PIAAWLAGLG LFTKRSHEKC 610 620 630 640 650 660 VPEAVFRAPN DQVALFLRHL WSAGGSVRWD PTNGQGRVYY GSTSRRLIDD VAQLLLRVGI 670 680 690 700 710 720 FSWITHAPKL GGHDSWRLHI HGAKDQVRFL RHVGVHGAEA VAAQEMLRQL KGPVRNPNLD 730 740 750 760 770 780 SAPKKVWAQV RNRLSAKQMM DIQLHEPTMW KHSPSRSRPH RAEARIEDRA IHELARGDAY 790 800 810 820 830 840 WDTVVEITSI GDQHVFDGTV SGTHNFVANG ISLHNSLEQD ADVVILLHRP DAFDRDDPRG 850 860 870 GEADFILAKH RNGPTKTVTV AHQLHLSRFA NMAR

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

BX842572 Genomic DNA. Translation: CAB02519.1.
AE000516 Genomic DNA. Translation: AAK44286.1.

PIR

B70914.

RefSeq

NP_214572.1.
NP_334472.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2R5U	X-ray	1.90	A/B/C/D/E/F	1-197	[»]

ModBase

Search...

Genome annotation databases

GeneID

887009.
922737.

GenomeReviews

Gene locus MT0064 in contig AE000516_GR.
Gene locus Rv0058 in contig AL123456_GR.

KEGG

mtc:MT0064.
mtu:Rv0058.

TIGR

MT0064.

Organism-specific databases

TubercuList

Rv0058.

Phylogenomic databases

HOGENOM

HBG734566.

OMA

WDEIVSI.

Family and domain databases

InterPro

IPR003593. ATPase_AAA+_core.
IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR003586. Hedgehog_hint_C.
IPR003587. Hedgehog_hint_N.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splicing_site.
[Graphical view]

Gene3D

G3DSA:1.10.860.10. DnaB_N. 1 hit.

Pfam

PF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view]

PRINTS

PR00379. INTEIN.

SMART

SM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]

PROSITE

PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DNAB_MYCTU

Accession

Primary (citable) accession number: P71715

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	December 15, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)