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Protein

FHA domain-containing protein FhaA

Gene

fhaA

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates cell growth and peptidoglycan synthesis by binding to MviN. May inhibit the late stages of peptidoglycan synthesis.1 Publication

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-4133-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
FHA domain-containing protein FhaA
Gene namesi
Name:fhaA
Synonyms:TB39.8
Ordered Locus Names:Rv0020c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv0020c.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi116T → A: Lack of phosphorylation. 1 Publication1
Mutagenesisi459R → A: Abolishes phosphorylation by PknB. Strong decrease in affinity for MviN. 2 Publications1
Mutagenesisi461Q → A: Does not affect affinity for MviN. 1 Publication1
Mutagenesisi473S → A: Abolishes phosphorylation by PknB. 1 Publication1
Mutagenesisi474R → A: Decrease in affinity for MviN. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004196641 – 527FHA domain-containing protein FhaAAdd BLAST527

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei116Phosphothreonine1 Publication1

Post-translational modificationi

Phosphorylated by PknB.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP71590.

PTM databases

iPTMnetiP71590.

Interactioni

Subunit structurei

Interacts with (phosphorylated) MviN and (phosphorylated) PknB via the FHA domain. Binds to the PknB juxtamembrane domain with an affinity that is modulated by the degree and the pattern of phosphorylation of this juxtamembrane domain.2 Publications

Protein-protein interaction databases

DIPiDIP-59047N.
STRINGi83332.Rv0020c.

Structurei

Secondary structure

1527
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi22 – 27Combined sources6
Helixi34 – 46Combined sources13
Beta strandi61 – 67Combined sources7
Helixi68 – 74Combined sources7
Helixi79 – 96Combined sources18
Beta strandi105 – 111Combined sources7
Beta strandi119 – 124Combined sources6
Beta strandi127 – 129Combined sources3
Beta strandi434 – 440Combined sources7
Beta strandi442 – 444Combined sources3
Beta strandi447 – 449Combined sources3
Beta strandi452 – 460Combined sources9
Beta strandi464 – 466Combined sources3
Beta strandi468 – 471Combined sources4
Beta strandi477 – 481Combined sources5
Beta strandi486 – 490Combined sources5
Beta strandi497 – 499Combined sources3
Beta strandi505 – 508Combined sources4
Beta strandi514 – 517Combined sources4
Beta strandi520 – 527Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LC0NMR-A1-132[»]
2LC1NMR-A430-527[»]
3OUNX-ray2.70A390-525[»]
3PO8X-ray1.50A431-527[»]
3POAX-ray2.01A431-527[»]
ProteinModelPortaliP71590.
SMRiP71590.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP71590.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini455 – 504FHAPROSITE-ProRule annotationAdd BLAST50

Domaini

The structure of the N-terminal domain remains unchanged upon phosphorylation.1 Publication

Sequence similaritiesi

Contains 1 FHA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105TV9. Bacteria.
COG1716. LUCA.
HOGENOMiHOG000235804.
InParanoidiP71590.
OMAiPQSYEQR.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR022128. DUF3662.
IPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PfamiPF12401. DUF3662. 1 hit.
PF00498. FHA. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P71590-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSQKRLVQR VERKLEQTVG DAFARIFGGS IVPQEVEALL RREAADGIQS
60 70 80 90 100
LQGNRLLAPN EYIITLGVHD FEKLGADPEL KSTGFARDLA DYIQEQGWQT
110 120 130 140 150
YGDVVVRFEQ SSNLHTGQFR ARGTVNPDVE THPPVIDCAR PQSNHAFGAE
160 170 180 190 200
PGVAPMSDNS SYRGGQGQGR PDEYYDDRYA RPQEDPRGGP DPQGGSDPRG
210 220 230 240 250
GYPPETGGYP PQPGYPRPRH PDQGDYPEQI GYPDQGGYPE QRGYPEQRGY
260 270 280 290 300
PDQRGYQDQG RGYPDQGQGG YPPPYEQRPP VSPGPAAGYG APGYDQGYRQ
310 320 330 340 350
SGGYGPSPGG GQPGYGGYGE YGRGPARHEE GSYVPSGPPG PPEQRPAYPD
360 370 380 390 400
QGGYDQGYQQ GATTYGRQDY GGGADYTRYT ESPRVPGYAP QGGGYAEPAG
410 420 430 440 450
RDYDYGQSGA PDYGQPAPGG YSGYGQGGYG SAGTSVTLQL DDGSGRTYQL
460 470 480 490 500
REGSNIIGRG QDAQFRLPDT GVSRRHLEIR WDGQVALLAD LNSTNGTTVN
510 520
NAPVQEWQLA DGDVIRLGHS EIIVRMH
Length:527
Mass (Da):56,880
Last modified:February 1, 1997 - v1
Checksum:i2642F9CF23FE7906
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP42742.1.
PIRiB70700.
RefSeqiNP_214534.1. NC_000962.3.
WP_003912368.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP42742; CCP42742; Rv0020c.
GeneIDi887067.
KEGGimtu:Rv0020c.
PATRICi18148538. VBIMycTub87468_0024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP42742.1.
PIRiB70700.
RefSeqiNP_214534.1. NC_000962.3.
WP_003912368.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LC0NMR-A1-132[»]
2LC1NMR-A430-527[»]
3OUNX-ray2.70A390-525[»]
3PO8X-ray1.50A431-527[»]
3POAX-ray2.01A431-527[»]
ProteinModelPortaliP71590.
SMRiP71590.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59047N.
STRINGi83332.Rv0020c.

PTM databases

iPTMnetiP71590.

Proteomic databases

PaxDbiP71590.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP42742; CCP42742; Rv0020c.
GeneIDi887067.
KEGGimtu:Rv0020c.
PATRICi18148538. VBIMycTub87468_0024.

Organism-specific databases

TubercuListiRv0020c.

Phylogenomic databases

eggNOGiENOG4105TV9. Bacteria.
COG1716. LUCA.
HOGENOMiHOG000235804.
InParanoidiP71590.
OMAiPQSYEQR.

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-4133-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP71590.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR022128. DUF3662.
IPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PfamiPF12401. DUF3662. 1 hit.
PF00498. FHA. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFHAA_MYCTU
AccessioniPrimary (citable) accession number: P71590
Secondary accession number(s): F2GPM4, L0T599
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.