P71554 (PUR3_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylglycinamide formyltransferase
EC=2.1.2.2

Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name=GART
Glycinamide ribonucleotide transformylase

Gene names

Name:	purN
Ordered Locus Names:	MT0983, Rv0956

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Subunit structure	Homodimer. Ref.3
Sequence similarities	Belongs to the GART family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway tetrahydrofolate metabolic process Inferred from direct assay Ref.3. Source: MTBBASE
Molecular_function	magnesium ion binding Inferred from direct assay Ref.3. Source: MTBBASE phosphoribosylglycinamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 215

215

Phosphoribosylglycinamide formyltransferase

PRO_0000420698

Regions

Region

23 – 25

3

5'-phosphoribosylglycinamide binding By similarity

Region

99 – 102

4

10-formyltetrahydrofolate binding By similarity

Region

100 – 102

3

10-formyltetrahydrofolate

Region

150 – 154

5

10-formyltetrahydrofolate

Sites

Active site

118

1

Proton donor By similarity

Metal binding

116

1

Magnesium

Metal binding

117

1

Magnesium

Binding site

116

1

10-formyltetrahydrofolate By similarity

Binding site

183

1

5'-phosphoribosylglycinamide By similarity

Site

154

1

Raises pKa of active site His By similarity

Secondary structure

1

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215

Helix Strand Turn

Details...

Beta strand

5 – 7

3

Beta strand

11 – 21

11

Helix

24 – 32

9

Beta strand

38 – 48

11

Helix

51 – 58

8

Beta strand

63 – 65

3

Helix

68 – 70

3

Beta strand

71 – 73

3

Helix

74 – 86

13

Beta strand

91 – 97

7

Helix

104 – 110

7

Turn

111 – 113

3

Beta strand

114 – 120

7

Helix

130 – 137

8

Beta strand

140 – 148

9

Beta strand

151 – 154

4

Beta strand

158 – 165

8

Helix

172 – 197

26

Beta strand

199 – 202

4

Beta strand

205 – 208

4

Sequences

	Sequence		Length	Mass (Da)
	P71554 [UniParc]. Last modified July 1, 1997. Version 2. Checksum: 55556C033097EAA1	FASTA	215	22,416
	10 20 30 40 50 60 MQEPLRVPPS APARLVVLAS GTGSLLRSLL DAAVGDYPAR VVAVGVDREC RAAEIAAEAS 70 80 90 100 110 120 VPVFTVRLAD HPSRDAWDVA ITAATAAHEP DLVVSAGFMR ILGPQFLSRF YGRTLNTHPA 130 140 150 160 170 180 LLPAFPGTHG VADALAYGVK VTGATVHLVD AGTDTGPILA QQPVPVLDGD DEETLHERIK 190 200 210 VTERRLLVAA VAALATHGVT VVGRTATMGR KVTIG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Structures of glycinamide ribonucleotide transformylase (PurN) from Mycobacterium tuberculosis reveal a novel dimer with relevance to drug discovery." Zhang Z., Caradoc-Davies T.T., Dickson J.M., Baker E.N., Squire C.J. J. Mol. Biol. 389:722-733(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) OF 2-215 IN COMPLEX WITH SUBSTRATE ANALOGS AND MAGNESIUM IONS, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000516 Genomic DNA. Translation: AAK45231.1.
AL123456 Genomic DNA. Translation: CCP43704.1.

PIR

B70717.

RefSeq

NP_215471.1. NC_000962.3.
NP_335417.1. NC_002755.2.
YP_006514313.1. NC_018143.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DA8	X-ray	1.30	A/B	2-215	[»]
3DCJ	X-ray	2.20	A/B	2-215	[»]

ProteinModelPortal

P71554.

SMR

P71554. Positions 3-215.

ModBase

Search...

Protein-protein interaction databases

STRING

83332.Rv0956.

Proteomic databases

PRIDE

P71554.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAK45231; AAK45231; MT0983.

GeneID

13319513.
885407.
926375.

KEGG

mtc:MT0983.
mtu:Rv0956.

PATRIC

18123906. VBIMycTub22151_1076.

Organism-specific databases

TubercuList

Rv0956.

Phylogenomic databases

HOGENOM

HOG000033575.

KO

K11175.

OMA

FIDACGN.

ProtClustDB

PRK05647.

Enzyme and pathway databases

UniPathway

UPA00074; UER00126.

Family and domain databases

Gene3D

3.40.50.170. 1 hit.

InterPro

IPR002376. Formyl_transf_N.
IPR004607. PurN_trans.
[Graphical view]

Pfam

PF00551. Formyl_trans_N. 1 hit.
[Graphical view]

SUPFAM

SSF53328. formyl_transf. 1 hit.

TIGRFAMs

TIGR00639. PurN. 1 hit.

PROSITE

PS00373. GART. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P71554.

Entry information

Entry name

PUR3_MYCTU

Accession

Primary (citable) accession number: P71554
Secondary accession number(s): F2GHX1, L0T6W9, Q7D921

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2013
Last sequence update:	July 1, 1997
Last modified:	May 29, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program