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Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.By similarity

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei55NADPBy similarity1
Binding sitei96NADP; via carbonyl oxygenBy similarity1
Binding sitei148SubstrateBy similarity1
Active sitei161Proton acceptorPROSITE-ProRule annotation1
Binding sitei194NADP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 33NADPBy similarity4
Nucleotide bindingi69 – 70NADPBy similarity2
Nucleotide bindingi161 – 165NADPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00094

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase
Gene namesi
Name:fabG
Ordered Locus Names:MSMEG_3150, MSMEI_3069
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000546741 – 2553-oxoacyl-[acyl-carrier-protein] reductase FabGAdd BLAST255

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_3150

Structurei

3D structure databases

ProteinModelPortaliP71534
SMRiP71534
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CHR Bacteria
ENOG410XNW1 LUCA
KOiK11610
OMAiGSRNIRC
OrthoDBiPOG091H012M

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR020904 Sc_DH/Rdtase_CS
IPR002347 SDR_fam
PRINTSiPR00081 GDHRDH
PR00080 SDRFAMILY
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00061 ADH_SHORT, 1 hit

Sequencei

Sequence statusi: Complete.

P71534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVTDNPADT AGEATAGRPA FVSRSVLVTG GNRGIGLAIA RRLAADGHKV
60 70 80 90 100
AVTHRGSGAP DDLFGVQCDV TDSAAVDRAF KEVEEHQGPV EVLVANAGIS
110 120 130 140 150
KDAFLMRMTE ERFEEVINTN LTGAFRCAQR ASRTMQRKRF GRIIFIGSVS
160 170 180 190 200
GMWGIGNQAN YAAAKAGLIG MARSISRELA KAGVTANVVA PGYIDTEMTR
210 220 230 240 250
ALDERIQAGA LDFIPAKRVG TAEEVAGAVS FLASEDASYI AGAVIPVDGG

MGMGH
Length:255
Mass (Da):26,641
Last modified:July 10, 2007 - v2
Checksum:iBAE0131C09C472DB
GO

Sequence cautioni

The sequence AFP39533 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75A → G in AAC69638 (Ref. 1) Curated1
Sequence conflicti180A → D in AAC69638 (Ref. 1) Curated1
Sequence conflicti189 – 190VA → LP in AAC69638 (Ref. 1) Curated2
Sequence conflicti208A → G in AAC69638 (Ref. 1) Curated1
Sequence conflicti211L → I in AAC69638 (Ref. 1) Curated1
Sequence conflicti216A → D in AAC69638 (Ref. 1) Curated1
Sequence conflicti222A → V in AAC69638 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66800 Genomic DNA Translation: AAC69638.1
CP000480 Genomic DNA Translation: ABK71816.1
CP001663 Genomic DNA Translation: AFP39533.1 Different initiation.
RefSeqiWP_011728850.1, NZ_CP009494.1
YP_887465.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK71816; ABK71816; MSMEG_3150
AFP39533; AFP39533; MSMEI_3069
GeneIDi4534094
KEGGimsb:LJ00_15665
msg:MSMEI_3069
msm:MSMEG_3150
PATRICifig|246196.19.peg.3111

Similar proteinsi

Entry informationi

Entry nameiFABG_MYCS2
AccessioniPrimary (citable) accession number: P71534
Secondary accession number(s): A0QX27, I7FLD4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 10, 2007
Last modified: March 28, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health