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P71534

- FABG_MYCS2

UniProt

P71534 - FABG_MYCS2

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Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene
fabG, MSMEG_3150, MSMEI_3069
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis By similarity.

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551NADP By similarity
Binding sitei96 – 961NADP; via carbonyl oxygen By similarity
Binding sitei148 – 1481Substrate By similarity
Active sitei161 – 1611Proton acceptor By similarity
Binding sitei194 – 1941NADP; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi30 – 334NADP By similarity
Nucleotide bindingi69 – 702NADP By similarity
Nucleotide bindingi161 – 1655NADP By similarity

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB
  2. NADP binding Source: UniProtKB

GO - Biological processi

  1. fatty acid elongation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3150-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase
Gene namesi
Name:fabG
Ordered Locus Names:MSMEG_3150, MSMEI_3069
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2552553-oxoacyl-[acyl-carrier-protein] reductase FabGPRO_0000054674Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi246196.MSMEG_3150.

Structurei

3D structure databases

ProteinModelPortaliP71534.
SMRiP71534. Positions 17-255.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
KOiK11610.
OMAiDTATENT.
OrthoDBiEOG6N3CR8.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P71534-1 [UniParc]FASTAAdd to Basket

« Hide

MTVTDNPADT AGEATAGRPA FVSRSVLVTG GNRGIGLAIA RRLAADGHKV    50
AVTHRGSGAP DDLFGVQCDV TDSAAVDRAF KEVEEHQGPV EVLVANAGIS 100
KDAFLMRMTE ERFEEVINTN LTGAFRCAQR ASRTMQRKRF GRIIFIGSVS 150
GMWGIGNQAN YAAAKAGLIG MARSISRELA KAGVTANVVA PGYIDTEMTR 200
ALDERIQAGA LDFIPAKRVG TAEEVAGAVS FLASEDASYI AGAVIPVDGG 250
MGMGH 255
Length:255
Mass (Da):26,641
Last modified:July 10, 2007 - v2
Checksum:iBAE0131C09C472DB
GO

Sequence cautioni

The sequence AFP39533.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 751A → G in AAC69638. 1 Publication
Sequence conflicti180 – 1801A → D in AAC69638. 1 Publication
Sequence conflicti189 – 1902VA → LP in AAC69638. 1 Publication
Sequence conflicti208 – 2081A → G in AAC69638. 1 Publication
Sequence conflicti211 – 2111L → I in AAC69638. 1 Publication
Sequence conflicti216 – 2161A → D in AAC69638. 1 Publication
Sequence conflicti222 – 2221A → V in AAC69638. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U66800 Genomic DNA. Translation: AAC69638.1.
CP000480 Genomic DNA. Translation: ABK71816.1.
CP001663 Genomic DNA. Translation: AFP39533.1. Different initiation.
RefSeqiWP_011728850.1. NC_008596.1.
YP_006567828.1. NC_018289.1.
YP_887465.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71816; ABK71816; MSMEG_3150.
AFP39533; AFP39533; MSMEI_3069.
GeneIDi4534094.
KEGGimsg:MSMEI_3069.
msm:MSMEG_3150.
PATRICi18078726. VBIMycSme59918_3111.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U66800 Genomic DNA. Translation: AAC69638.1 .
CP000480 Genomic DNA. Translation: ABK71816.1 .
CP001663 Genomic DNA. Translation: AFP39533.1 . Different initiation.
RefSeqi WP_011728850.1. NC_008596.1.
YP_006567828.1. NC_018289.1.
YP_887465.1. NC_008596.1.

3D structure databases

ProteinModelPortali P71534.
SMRi P71534. Positions 17-255.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_3150.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK71816 ; ABK71816 ; MSMEG_3150 .
AFP39533 ; AFP39533 ; MSMEI_3069 .
GeneIDi 4534094.
KEGGi msg:MSMEI_3069.
msm:MSMEG_3150.
PATRICi 18078726. VBIMycSme59918_3111.

Phylogenomic databases

eggNOGi COG1028.
KOi K11610.
OMAi DTATENT.
OrthoDBi EOG6N3CR8.

Enzyme and pathway databases

UniPathwayi UPA00094 .
BioCyci MSME246196:GJ4Y-3150-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, expression and characterization of 3-ketoacyl reductase from mycobacteria."
    Banerjee A., Sugantino M., Sacchettini J.C., Jacobs W.R. Jr.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiFABG_MYCS2
AccessioniPrimary (citable) accession number: P71534
Secondary accession number(s): A0QX27, I7FLD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 10, 2007
Last modified: September 3, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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