Reviewed, UniProtKB/Swiss-Prot P71534 (FABG_MYCS2)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] reductase
    EC=1.1.1.100
Alternative name(s):
    3-ketoacyl-acyl carrier protein reductase

Gene names

Name:	fabG
Ordered Locus Names:	MSMEG_3150

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Complete proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-oxoacyl-[acyl-carrier-protein] reductase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	3-oxoacyl-[acyl-carrier-protein] reductase		PRO_0000054674
Regions
	Nucleotide binding	10 – 39	30	NADP By similarity
Sites
	Active site	161	1	Proton acceptor By similarity
	Binding site	148	1	Substrate By similarity
Experimental info
	Sequence conflict	75	1	A → G in AAC69638. Ref.1
	Sequence conflict	180	1	A → D in AAC69638. Ref.1
	Sequence conflict	189 – 190	2	VA → LP in AAC69638. Ref.1
	Sequence conflict	208	1	A → G in AAC69638. Ref.1
	Sequence conflict	211	1	L → I in AAC69638. Ref.1
	Sequence conflict	216	1	A → D in AAC69638. Ref.1
	Sequence conflict	222	1	A → V in AAC69638. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P71534-1 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: BAE0131C09C472DB	FASTA	255	26,641
	10 20 30 40 50 60 MTVTDNPADT AGEATAGRPA FVSRSVLVTG GNRGIGLAIA RRLAADGHKV AVTHRGSGAP 70 80 90 100 110 120 DDLFGVQCDV TDSAAVDRAF KEVEEHQGPV EVLVANAGIS KDAFLMRMTE ERFEEVINTN 130 140 150 160 170 180 LTGAFRCAQR ASRTMQRKRF GRIIFIGSVS GMWGIGNQAN YAAAKAGLIG MARSISRELA 190 200 210 220 230 240 KAGVTANVVA PGYIDTEMTR ALDERIQAGA LDFIPAKRVG TAEEVAGAVS FLASEDASYI 250 AGAVIPVDGG MGMGH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, expression and characterization of 3-ketoacyl reductase from mycobacteria." Banerjee A., Sugantino M., Sacchettini J.C., Jacobs W.R. Jr. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U66800 Genomic DNA. Translation: AAC69638.1. CP000480 Genomic DNA. Translation: ABK71816.1.
RefSeq	YP_887465.1.
3D structure databases
HSSP	HSSP built from PDB template 1EDO based on UniProtKB Q93X62.
SMR	P71534. Positions 17-255.
ModBase	Search...
Protein-protein interaction databases
STRING	P71534.
Genome annotation databases
GeneID	4534094.
GenomeReviews	Gene locus MSMEG_3150 in contig CP000480_GR.
KEGG	msm:MSMEG_3150.
NMPDR	fig|246196.1.peg.3158.
TIGR	MSMEG_3150.
Phylogenomic databases
OMA	CIAETLA.
Family and domain databases
InterPro	IPR011284. 3oxo_ACP_reduc. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FABG_MYCS2

Accession

Primary (citable) accession number: P71534
Secondary accession number(s): A0QX27

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 10, 2007
Last modified:	November 3, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)