Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.By similarity

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei55NADPBy similarity1
Binding sitei96NADP; via carbonyl oxygenBy similarity1
Binding sitei148SubstrateBy similarity1
Active sitei161Proton acceptorPROSITE-ProRule annotation1
Binding sitei194NADP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 33NADPBy similarity4
Nucleotide bindingi69 – 70NADPBy similarity2
Nucleotide bindingi161 – 165NADPBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase
Gene namesi
Name:fabG
Ordered Locus Names:MSMEG_3150, MSMEI_3069
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000546741 – 2553-oxoacyl-[acyl-carrier-protein] reductase FabGAdd BLAST255

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_3150.

Structurei

3D structure databases

ProteinModelPortaliP71534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CHR. Bacteria.
ENOG410XNW1. LUCA.
KOiK11610.
OMAiKRATRSM.
OrthoDBiPOG091H012M.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P71534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVTDNPADT AGEATAGRPA FVSRSVLVTG GNRGIGLAIA RRLAADGHKV
60 70 80 90 100
AVTHRGSGAP DDLFGVQCDV TDSAAVDRAF KEVEEHQGPV EVLVANAGIS
110 120 130 140 150
KDAFLMRMTE ERFEEVINTN LTGAFRCAQR ASRTMQRKRF GRIIFIGSVS
160 170 180 190 200
GMWGIGNQAN YAAAKAGLIG MARSISRELA KAGVTANVVA PGYIDTEMTR
210 220 230 240 250
ALDERIQAGA LDFIPAKRVG TAEEVAGAVS FLASEDASYI AGAVIPVDGG

MGMGH
Length:255
Mass (Da):26,641
Last modified:July 10, 2007 - v2
Checksum:iBAE0131C09C472DB
GO

Sequence cautioni

The sequence AFP39533 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75A → G in AAC69638 (Ref. 1) Curated1
Sequence conflicti180A → D in AAC69638 (Ref. 1) Curated1
Sequence conflicti189 – 190VA → LP in AAC69638 (Ref. 1) Curated2
Sequence conflicti208A → G in AAC69638 (Ref. 1) Curated1
Sequence conflicti211L → I in AAC69638 (Ref. 1) Curated1
Sequence conflicti216A → D in AAC69638 (Ref. 1) Curated1
Sequence conflicti222A → V in AAC69638 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66800 Genomic DNA. Translation: AAC69638.1.
CP000480 Genomic DNA. Translation: ABK71816.1.
CP001663 Genomic DNA. Translation: AFP39533.1. Different initiation.
RefSeqiWP_011728850.1. NZ_CP009494.1.
YP_887465.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71816; ABK71816; MSMEG_3150.
AFP39533; AFP39533; MSMEI_3069.
GeneIDi4534094.
KEGGimsb:LJ00_15665.
msg:MSMEI_3069.
msm:MSMEG_3150.
PATRICi18078726. VBIMycSme59918_3111.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66800 Genomic DNA. Translation: AAC69638.1.
CP000480 Genomic DNA. Translation: ABK71816.1.
CP001663 Genomic DNA. Translation: AFP39533.1. Different initiation.
RefSeqiWP_011728850.1. NZ_CP009494.1.
YP_887465.1. NC_008596.1.

3D structure databases

ProteinModelPortaliP71534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_3150.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK71816; ABK71816; MSMEG_3150.
AFP39533; AFP39533; MSMEI_3069.
GeneIDi4534094.
KEGGimsb:LJ00_15665.
msg:MSMEI_3069.
msm:MSMEG_3150.
PATRICi18078726. VBIMycSme59918_3111.

Phylogenomic databases

eggNOGiENOG4105CHR. Bacteria.
ENOG410XNW1. LUCA.
KOiK11610.
OMAiKRATRSM.
OrthoDBiPOG091H012M.

Enzyme and pathway databases

UniPathwayiUPA00094.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFABG_MYCS2
AccessioniPrimary (citable) accession number: P71534
Secondary accession number(s): A0QX27, I7FLD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 10, 2007
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.