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P71534

- FABG_MYCS2

UniProt

P71534 - FABG_MYCS2

Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.By similarity

    Catalytic activityi

    (3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei55 – 551NADPBy similarity
    Binding sitei96 – 961NADP; via carbonyl oxygenBy similarity
    Binding sitei148 – 1481SubstrateBy similarity
    Active sitei161 – 1611Proton acceptorPROSITE-ProRule annotation
    Binding sitei194 – 1941NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi30 – 334NADPBy similarity
    Nucleotide bindingi69 – 702NADPBy similarity
    Nucleotide bindingi161 – 1655NADPBy similarity

    GO - Molecular functioni

    1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB
    2. NADP binding Source: UniProtKB

    GO - Biological processi

    1. fatty acid elongation Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-3150-MONOMER.
    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
    Alternative name(s):
    3-ketoacyl-acyl carrier protein reductase
    Beta-Ketoacyl-acyl carrier protein reductase
    Beta-ketoacyl-ACP reductase
    Gene namesi
    Name:fabG
    Ordered Locus Names:MSMEG_3150, MSMEI_3069
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2552553-oxoacyl-[acyl-carrier-protein] reductase FabGPRO_0000054674Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi246196.MSMEG_3150.

    Structurei

    3D structure databases

    ProteinModelPortaliP71534.
    SMRiP71534. Positions 17-255.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1028.
    KOiK11610.
    OMAiDTATENT.
    OrthoDBiEOG6N3CR8.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P71534-1 [UniParc]FASTAAdd to Basket

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    MTVTDNPADT AGEATAGRPA FVSRSVLVTG GNRGIGLAIA RRLAADGHKV    50
    AVTHRGSGAP DDLFGVQCDV TDSAAVDRAF KEVEEHQGPV EVLVANAGIS 100
    KDAFLMRMTE ERFEEVINTN LTGAFRCAQR ASRTMQRKRF GRIIFIGSVS 150
    GMWGIGNQAN YAAAKAGLIG MARSISRELA KAGVTANVVA PGYIDTEMTR 200
    ALDERIQAGA LDFIPAKRVG TAEEVAGAVS FLASEDASYI AGAVIPVDGG 250
    MGMGH 255
    Length:255
    Mass (Da):26,641
    Last modified:July 10, 2007 - v2
    Checksum:iBAE0131C09C472DB
    GO

    Sequence cautioni

    The sequence AFP39533.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti75 – 751A → G in AAC69638. 1 PublicationCurated
    Sequence conflicti180 – 1801A → D in AAC69638. 1 PublicationCurated
    Sequence conflicti189 – 1902VA → LP in AAC69638. 1 PublicationCurated
    Sequence conflicti208 – 2081A → G in AAC69638. 1 PublicationCurated
    Sequence conflicti211 – 2111L → I in AAC69638. 1 PublicationCurated
    Sequence conflicti216 – 2161A → D in AAC69638. 1 PublicationCurated
    Sequence conflicti222 – 2221A → V in AAC69638. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U66800 Genomic DNA. Translation: AAC69638.1.
    CP000480 Genomic DNA. Translation: ABK71816.1.
    CP001663 Genomic DNA. Translation: AFP39533.1. Different initiation.
    RefSeqiWP_011728850.1. NC_008596.1.
    YP_006567828.1. NC_018289.1.
    YP_887465.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK71816; ABK71816; MSMEG_3150.
    AFP39533; AFP39533; MSMEI_3069.
    GeneIDi4534094.
    KEGGimsg:MSMEI_3069.
    msm:MSMEG_3150.
    PATRICi18078726. VBIMycSme59918_3111.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U66800 Genomic DNA. Translation: AAC69638.1 .
    CP000480 Genomic DNA. Translation: ABK71816.1 .
    CP001663 Genomic DNA. Translation: AFP39533.1 . Different initiation.
    RefSeqi WP_011728850.1. NC_008596.1.
    YP_006567828.1. NC_018289.1.
    YP_887465.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali P71534.
    SMRi P71534. Positions 17-255.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_3150.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK71816 ; ABK71816 ; MSMEG_3150 .
    AFP39533 ; AFP39533 ; MSMEI_3069 .
    GeneIDi 4534094.
    KEGGi msg:MSMEI_3069.
    msm:MSMEG_3150.
    PATRICi 18078726. VBIMycSme59918_3111.

    Phylogenomic databases

    eggNOGi COG1028.
    KOi K11610.
    OMAi DTATENT.
    OrthoDBi EOG6N3CR8.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .
    BioCyci MSME246196:GJ4Y-3150-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, expression and characterization of 3-ketoacyl reductase from mycobacteria."
      Banerjee A., Sugantino M., Sacchettini J.C., Jacobs W.R. Jr.
      Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.

    Entry informationi

    Entry nameiFABG_MYCS2
    AccessioniPrimary (citable) accession number: P71534
    Secondary accession number(s): A0QX27, I7FLD4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3