Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.By similarity

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551NADPBy similarity
Binding sitei96 – 961NADP; via carbonyl oxygenBy similarity
Binding sitei148 – 1481SubstrateBy similarity
Active sitei161 – 1611Proton acceptorPROSITE-ProRule annotation
Binding sitei194 – 1941NADP; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi30 – 334NADPBy similarity
Nucleotide bindingi69 – 702NADPBy similarity
Nucleotide bindingi161 – 1655NADPBy similarity

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB
  2. NADP binding Source: UniProtKB

GO - Biological processi

  1. fatty acid elongation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3150-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase
Gene namesi
Name:fabG
Ordered Locus Names:MSMEG_3150, MSMEI_3069
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2552553-oxoacyl-[acyl-carrier-protein] reductase FabGPRO_0000054674Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_3150.

Structurei

3D structure databases

ProteinModelPortaliP71534.
SMRiP71534. Positions 17-255.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
KOiK11610.
OMAiCAKRAST.
OrthoDBiEOG6N3CR8.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P71534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVTDNPADT AGEATAGRPA FVSRSVLVTG GNRGIGLAIA RRLAADGHKV
60 70 80 90 100
AVTHRGSGAP DDLFGVQCDV TDSAAVDRAF KEVEEHQGPV EVLVANAGIS
110 120 130 140 150
KDAFLMRMTE ERFEEVINTN LTGAFRCAQR ASRTMQRKRF GRIIFIGSVS
160 170 180 190 200
GMWGIGNQAN YAAAKAGLIG MARSISRELA KAGVTANVVA PGYIDTEMTR
210 220 230 240 250
ALDERIQAGA LDFIPAKRVG TAEEVAGAVS FLASEDASYI AGAVIPVDGG

MGMGH
Length:255
Mass (Da):26,641
Last modified:July 10, 2007 - v2
Checksum:iBAE0131C09C472DB
GO

Sequence cautioni

The sequence AFP39533.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 751A → G in AAC69638 (Ref. 1) Curated
Sequence conflicti180 – 1801A → D in AAC69638 (Ref. 1) Curated
Sequence conflicti189 – 1902VA → LP in AAC69638 (Ref. 1) Curated
Sequence conflicti208 – 2081A → G in AAC69638 (Ref. 1) Curated
Sequence conflicti211 – 2111L → I in AAC69638 (Ref. 1) Curated
Sequence conflicti216 – 2161A → D in AAC69638 (Ref. 1) Curated
Sequence conflicti222 – 2221A → V in AAC69638 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66800 Genomic DNA. Translation: AAC69638.1.
CP000480 Genomic DNA. Translation: ABK71816.1.
CP001663 Genomic DNA. Translation: AFP39533.1. Different initiation.
RefSeqiYP_006567828.1. NC_018289.1.
YP_887465.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71816; ABK71816; MSMEG_3150.
AFP39533; AFP39533; MSMEI_3069.
GeneIDi4534094.
KEGGimsm:MSMEG_3150.
PATRICi18078726. VBIMycSme59918_3111.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66800 Genomic DNA. Translation: AAC69638.1.
CP000480 Genomic DNA. Translation: ABK71816.1.
CP001663 Genomic DNA. Translation: AFP39533.1. Different initiation.
RefSeqiYP_006567828.1. NC_018289.1.
YP_887465.1. NC_008596.1.

3D structure databases

ProteinModelPortaliP71534.
SMRiP71534. Positions 17-255.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_3150.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK71816; ABK71816; MSMEG_3150.
AFP39533; AFP39533; MSMEI_3069.
GeneIDi4534094.
KEGGimsm:MSMEG_3150.
PATRICi18078726. VBIMycSme59918_3111.

Phylogenomic databases

eggNOGiCOG1028.
KOiK11610.
OMAiCAKRAST.
OrthoDBiEOG6N3CR8.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciMSME246196:GJ4Y-3150-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, expression and characterization of 3-ketoacyl reductase from mycobacteria."
    Banerjee A., Sugantino M., Sacchettini J.C., Jacobs W.R. Jr.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiFABG_MYCS2
AccessioniPrimary (citable) accession number: P71534
Secondary accession number(s): A0QX27, I7FLD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 10, 2007
Last modified: March 4, 2015
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.