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Protein

Protein translocase subunit SecA 1

Gene

secA1

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi98 – 1058ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-1880-MONOMER.
BRENDAi3.6.3.B1. 3512.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocase subunit SecA 1UniRule annotation
Gene namesi
Name:secA1UniRule annotation
Ordered Locus Names:MSMEG_1881, MSMEI_1840
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Cytoplasm UniRule annotation

  • Note: Distribution is 50-50.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Essential, it cannot be disrupted.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953Protein translocase subunit SecA 1PRO_0000109597Add
BLAST

Interactioni

Subunit structurei

Monomer and homodimer. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_1881.

Structurei

3D structure databases

ProteinModelPortaliP71533.
SMRiP71533. Positions 1-832.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SecA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CI6. Bacteria.
COG0653. LUCA.
HOGENOMiHOG000218168.
KOiK03070.
OMAiIATERHE.
OrthoDBiEOG654P48.

Family and domain databases

Gene3Di1.10.3060.10. 1 hit.
3.40.50.300. 3 hits.
3.90.1440.10. 1 hit.
HAMAPiMF_01382. SecA.
InterProiIPR027417. P-loop_NTPase.
IPR000185. SecA.
IPR020937. SecA_CS.
IPR011115. SecA_DEAD.
IPR014018. SecA_motor_DEAD.
IPR011130. SecA_preprotein_X-link_dom.
IPR011116. SecA_Wing/Scaffold.
[Graphical view]
PfamiPF07517. SecA_DEAD. 1 hit.
PF01043. SecA_PP_bind. 1 hit.
PF07516. SecA_SW. 1 hit.
[Graphical view]
PRINTSiPR00906. SECA.
SMARTiSM00957. SecA_DEAD. 1 hit.
SM00958. SecA_PP_bind. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
SSF81767. SSF81767. 1 hit.
SSF81886. SSF81886. 1 hit.
TIGRFAMsiTIGR00963. secA. 1 hit.
PROSITEiPS01312. SECA. 1 hit.
PS51196. SECA_MOTOR_DEAD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P71533-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSKLLRLGE GRMVKRLRKV ADYVNALSDD VEKLSDAELR AKTEEFKKRV
60 70 80 90 100
ADGEDLDDLL PEAFAVAREA AWRVLNQRHF DVQVMGGAAL HFGNVAEMKT
110 120 130 140 150
GEGKTLTAVL PSYLNALSGK GVHVVTVNDY LARRDSEWMG RVHRFLGLDV
160 170 180 190 200
GVILSGMTPD ERRAAYAADI TYGTNNEFGF DYLRDNMAHS VDDMVQRGHN
210 220 230 240 250
FAIVDEVDSI LIDEARTPLI ISGPADGASH WYQEFARIVP MMEKDVHYEV
260 270 280 290 300
DLRKRTVGVH ELGVEFVEDQ LGIDNLYEAA NSPLVSYLNN ALKAKELFQR
310 320 330 340 350
DKDYIVRNGE VLIVDEFTGR VLMGRRYNEG MHQAIEAKER VEIKAENQTL
360 370 380 390 400
ATITLQNYFR LYDKLSGMTG TAETEAAELH EIYKLGVVPI PTNKPMVRQD
410 420 430 440 450
QSDLIYKTEE AKFLAVVDDV AERHAKGQPV LIGTTSVERS EYLSKMLTKR
460 470 480 490 500
RVPHNVLNAK YHEQEANIIA EAGRRGAVTV ATNMAGRGTD IVLGGNVDFL
510 520 530 540 550
ADKRLRERGL DPVETPEEYE AAWHEVLPQV KAECAKEAEQ VIEAGGLYVL
560 570 580 590 600
GTERHESRRI DNQLRGRSGR QGDPGESRFY LSLGDELMRR FNGATLETLL
610 620 630 640 650
TRLNLPDDVP IEAKMVSRAI KSAQTQVEQQ NFEVRKNVLK YDEVMNQQRK
660 670 680 690 700
VIYAERRRIL EGENLAEQAH KMLVDVITAY VDGATAEGYA EDWDLETLWT
710 720 730 740 750
ALKTLYPVGI DHRDLIDSDA VGEPGELTRE ELLDALIKDA ERAYAEREKQ
760 770 780 790 800
IEAIAGEGAM RQLERNVLLN VIDRKWREHL YEMDYLKEGI GLRAMAQRDP
810 820 830 840 850
LVEYQREGYD MFVGMLEALK EESVGFLFNV QVEAAPQQPQ VAPQAPPPTL
860 870 880 890 900
SEFAAAAAAK ASDSAAKPDS GSVATKERAE AERPAPALRA KGIDNEAPPL
910 920 930 940 950
TYTGPSEDGT AQVQRSGNGG RHAAPAGGSR RERREAARKQ AKADRPAKSH

RKG
Length:953
Mass (Da):106,488
Last modified:February 5, 2008 - v2
Checksum:i4D1AB93BC0752221
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti260 – 2601H → HVGVH in AAB06754 (PubMed:11717254).Curated
Sequence conflicti752 – 7521E → K in AAB06754 (PubMed:11717254).Curated
Sequence conflicti775 – 7751K → Q in AAB06754 (PubMed:11717254).Curated
Sequence conflicti908 – 9092DG → ER in AAB06754 (PubMed:11717254).Curated
Sequence conflicti912 – 9121Q → H in AAB06754 (PubMed:11717254).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66081 Genomic DNA. Translation: AAB06754.1.
CP000480 Genomic DNA. Translation: ABK74020.1.
CP001663 Genomic DNA. Translation: AFP38312.1.
RefSeqiWP_011727975.1. NZ_CP009494.1.
YP_886246.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK74020; ABK74020; MSMEG_1881.
AFP38312; AFP38312; MSMEI_1840.
GeneIDi4536291.
KEGGimsb:LJ00_09385.
msg:MSMEI_1840.
msm:MSMEG_1881.
PATRICi18076211. VBIMycSme59918_1862.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66081 Genomic DNA. Translation: AAB06754.1.
CP000480 Genomic DNA. Translation: ABK74020.1.
CP001663 Genomic DNA. Translation: AFP38312.1.
RefSeqiWP_011727975.1. NZ_CP009494.1.
YP_886246.1. NC_008596.1.

3D structure databases

ProteinModelPortaliP71533.
SMRiP71533. Positions 1-832.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_1881.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK74020; ABK74020; MSMEG_1881.
AFP38312; AFP38312; MSMEI_1840.
GeneIDi4536291.
KEGGimsb:LJ00_09385.
msg:MSMEI_1840.
msm:MSMEG_1881.
PATRICi18076211. VBIMycSme59918_1862.

Phylogenomic databases

eggNOGiENOG4105CI6. Bacteria.
COG0653. LUCA.
HOGENOMiHOG000218168.
KOiK03070.
OMAiIATERHE.
OrthoDBiEOG654P48.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-1880-MONOMER.
BRENDAi3.6.3.B1. 3512.

Family and domain databases

Gene3Di1.10.3060.10. 1 hit.
3.40.50.300. 3 hits.
3.90.1440.10. 1 hit.
HAMAPiMF_01382. SecA.
InterProiIPR027417. P-loop_NTPase.
IPR000185. SecA.
IPR020937. SecA_CS.
IPR011115. SecA_DEAD.
IPR014018. SecA_motor_DEAD.
IPR011130. SecA_preprotein_X-link_dom.
IPR011116. SecA_Wing/Scaffold.
[Graphical view]
PfamiPF07517. SecA_DEAD. 1 hit.
PF01043. SecA_PP_bind. 1 hit.
PF07516. SecA_SW. 1 hit.
[Graphical view]
PRINTSiPR00906. SECA.
SMARTiSM00957. SecA_DEAD. 1 hit.
SM00958. SecA_PP_bind. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
SSF81767. SSF81767. 1 hit.
SSF81886. SSF81886. 1 hit.
TIGRFAMsiTIGR00963. secA. 1 hit.
PROSITEiPS01312. SECA. 1 hit.
PS51196. SECA_MOTOR_DEAD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two nonredundant SecA homologues function in mycobacteria."
    Braunstein M., Brown A.M., Kurtz S., Jacobs W.R. Jr.
    J. Bacteriol. 183:6979-6990(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
    Strain: ATCC 700084 / mc(2)155.
  2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiSECA1_MYCS2
AccessioniPrimary (citable) accession number: P71533
Secondary accession number(s): A0QTK8, I7FYZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 5, 2008
Last modified: November 11, 2015
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.